Calcium in PDB 2nob: Structure of Catalytically Inactive H270A Human 8-Oxoguanine Glycosylase Crosslinked to 8-Oxoguanine Dna

All present enzymatic activity of Structure of Catalytically Inactive H270A Human 8-Oxoguanine Glycosylase Crosslinked to 8-Oxoguanine Dna:
4.2.99.18;

The structure of Structure of Catalytically Inactive H270A Human 8-Oxoguanine Glycosylase Crosslinked to 8-Oxoguanine Dna, PDB code: 2nob was solved by C.T.Radom, A.Banerjee, G.L.Verdine, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	50.00 / 2.10
Space group	P 65 2 2
Cell size a, b, c (Å), α, β, γ (°)	92.359, 92.359, 211.726, 90.00, 90.00, 120.00
R / Rfree (%)	22.5 / 25.8

The binding sites of Calcium atom in the Structure of Catalytically Inactive H270A Human 8-Oxoguanine Glycosylase Crosslinked to 8-Oxoguanine Dna (pdb code 2nob). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 2 binding sites of Calcium where determined in the Structure of Catalytically Inactive H270A Human 8-Oxoguanine Glycosylase Crosslinked to 8-Oxoguanine Dna, PDB code: 2nob:
Jump to Calcium binding site number: 1; 2;

Calcium binding site 1 out of 2 in the Structure of Catalytically Inactive H270A Human 8-Oxoguanine Glycosylase Crosslinked to 8-Oxoguanine Dna


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Structure of Catalytically Inactive H270A Human 8-Oxoguanine Glycosylase Crosslinked to 8-Oxoguanine Dna within 5.0Å range: probe atom residue distance (Å) B Occ C:Ca301 b:88.7 occ:1.00 OP1 C:DG26 3.2 35.7 1.0 C8 C:DA24 3.8 43.6 1.0 OP2 C:DG26 3.8 31.8 1.0 P C:DG26 3.9 34.2 1.0 N7 C:DA24 4.1 44.4 1.0 CG2 A:VAL269 4.2 35.8 1.0 OP2 C:DA24 4.5 70.5 1.0 O5' C:DG26 4.5 35.8 1.0 O C:HOH308 4.6 41.6 1.0 CA A:CYS149 4.7 34.0 1.0 C2' C:DA24 4.7 44.1 1.0 O C:HOH309 4.7 49.0 1.0 N7 C:DG26 4.8 38.1 1.0 N9 C:DA24 4.8 44.8 1.0 C8 C:DG26 4.8 38.0 1.0 SG A:CYS149 4.9 43.7 1.0

Calcium binding site 2 out of 2 in the Structure of Catalytically Inactive H270A Human 8-Oxoguanine Glycosylase Crosslinked to 8-Oxoguanine Dna


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of Structure of Catalytically Inactive H270A Human 8-Oxoguanine Glycosylase Crosslinked to 8-Oxoguanine Dna within 5.0Å range: probe atom residue distance (Å) B Occ C:Ca302 b:79.4 occ:1.00 O A:CYS241 2.7 37.4 1.0 OP1 C:DC28 2.7 45.6 1.0 O A:LEU243 2.8 43.1 1.0 O A:HOH492 2.9 56.9 1.0 O C:HOH311 3.0 55.9 1.0 O A:VAL246 3.2 38.2 1.0 OP2 C:DC28 3.3 46.5 1.0 P C:DC28 3.5 45.3 1.0 C A:LEU243 3.7 41.7 1.0 O A:ILE242 3.7 42.6 1.0 C A:CYS241 3.7 38.8 1.0 C A:ILE242 4.0 43.3 1.0 C A:VAL246 4.2 38.7 1.0 N A:VAL246 4.2 35.9 1.0 CA A:PRO244 4.3 40.6 1.0 N A:PRO244 4.3 41.7 1.0 N A:GLY245 4.4 41.3 1.0 N A:LEU243 4.4 43.1 1.0 O3' C:DT27 4.6 45.4 1.0 CA A:CYS241 4.6 37.8 1.0 N A:ILE242 4.6 40.6 1.0 O5' C:DC28 4.6 47.6 1.0 CA A:ILE242 4.6 43.8 1.0 CA A:VAL246 4.6 36.4 1.0 CA A:LEU243 4.7 42.8 1.0 C A:PRO244 4.7 41.6 1.0 CB A:VAL246 4.9 36.1 1.0

C.T.Radom, A.Banerjee, G.L.Verdine. Structural Characterization of Human 8-Oxoguanine Dna Glycosylase Variants Bearing Active Site Mutations. J.Biol.Chem. V. 282 9182 2007.
ISSN: ISSN 0021-9258
PubMed: 17114185
DOI: 10.1074/JBC.M608989200