Calcium in PDB 2noh: Structure of Catalytically Inactive Q315A Human 8-Oxoguanine Glycosylase Complexed to 8-Oxoguanine Dna

All present enzymatic activity of Structure of Catalytically Inactive Q315A Human 8-Oxoguanine Glycosylase Complexed to 8-Oxoguanine Dna:
4.2.99.18;

The structure of Structure of Catalytically Inactive Q315A Human 8-Oxoguanine Glycosylase Complexed to 8-Oxoguanine Dna, PDB code: 2noh was solved by C.T.Radom, A.Banerjee, G.L.Verdine, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	50.00 / 2.01
Space group	P 65 2 2
Cell size a, b, c (Å), α, β, γ (°)	92.169, 92.169, 211.382, 90.00, 90.00, 120.00
R / Rfree (%)	21.3 / 24.2

The binding sites of Calcium atom in the Structure of Catalytically Inactive Q315A Human 8-Oxoguanine Glycosylase Complexed to 8-Oxoguanine Dna (pdb code 2noh). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 2 binding sites of Calcium where determined in the Structure of Catalytically Inactive Q315A Human 8-Oxoguanine Glycosylase Complexed to 8-Oxoguanine Dna, PDB code: 2noh:
Jump to Calcium binding site number: 1; 2;

Calcium binding site 1 out of 2 in the Structure of Catalytically Inactive Q315A Human 8-Oxoguanine Glycosylase Complexed to 8-Oxoguanine Dna


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Structure of Catalytically Inactive Q315A Human 8-Oxoguanine Glycosylase Complexed to 8-Oxoguanine Dna within 5.0Å range: probe atom residue distance (Å) B Occ C:Ca401 b:49.6 occ:1.00 O C:HOH410 2.8 29.9 1.0 O C:HOH415 2.9 40.4 1.0 O C:HOH405 2.9 34.3 1.0 OP2 C:DA24 2.9 42.5 1.0 O C:HOH411 3.0 46.5 1.0 CG2 A:VAL269 4.1 27.8 1.0 C8 C:DA24 4.3 30.5 1.0 N7 C:DG26 4.3 28.9 1.0 P C:DA24 4.4 44.5 1.0 N7 C:DA24 4.4 31.6 1.0 OP1 C:DG26 4.6 25.5 1.0 OD1 A:ASN149 4.7 28.9 1.0 C2' C:DC23 4.7 49.7 1.0 O A:HOH388 4.9 33.9 1.0 C8 C:DG26 5.0 27.9 1.0

Calcium binding site 2 out of 2 in the Structure of Catalytically Inactive Q315A Human 8-Oxoguanine Glycosylase Complexed to 8-Oxoguanine Dna


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of Structure of Catalytically Inactive Q315A Human 8-Oxoguanine Glycosylase Complexed to 8-Oxoguanine Dna within 5.0Å range: probe atom residue distance (Å) B Occ C:Ca402 b:79.6 occ:1.00 O A:CYS241 2.7 28.0 1.0 OP1 C:DC28 2.8 36.0 1.0 O A:HOH579 2.9 48.7 1.0 O A:LEU243 3.0 32.6 1.0 O A:VAL246 3.0 28.2 1.0 OP2 C:DC28 3.0 35.2 1.0 P C:DC28 3.4 34.4 1.0 C A:CYS241 3.8 27.9 1.0 C A:LEU243 3.8 31.6 1.0 C A:VAL246 3.9 29.1 1.0 C A:ILE242 4.1 30.9 1.0 O A:ILE242 4.2 30.1 1.0 N A:VAL246 4.2 26.1 1.0 N A:LEU243 4.3 30.1 1.0 N A:PRO244 4.4 31.6 1.0 O3' C:DT27 4.5 35.0 1.0 CA A:PRO244 4.5 31.7 1.0 N A:GLY245 4.5 31.0 1.0 CA A:VAL246 4.5 26.1 1.0 CA A:ILE242 4.5 30.1 1.0 O5' C:DC28 4.6 37.9 1.0 N A:ILE242 4.6 28.5 1.0 CA A:CYS241 4.6 27.1 1.0 CA A:LEU243 4.6 30.5 1.0 C A:PRO244 4.8 31.9 1.0 CB A:VAL246 4.9 25.4 1.0 N A:GLY247 4.9 27.7 1.0

C.T.Radom, A.Banerjee, G.L.Verdine. Structural Characterization of Human 8-Oxoguanine Dna Glycosylase Variants Bearing Active Site Mutations. J.Biol.Chem. V. 282 9182 2007.
ISSN: ISSN 0021-9258
PubMed: 17114185
DOI: 10.1074/JBC.M608989200