Calcium in PDB 2nol: Structure of Catalytically Inactive Human 8-Oxoguanine Glycosylase Distal Crosslink to Oxog Dna

All present enzymatic activity of Structure of Catalytically Inactive Human 8-Oxoguanine Glycosylase Distal Crosslink to Oxog Dna:
4.2.99.18;

The structure of Structure of Catalytically Inactive Human 8-Oxoguanine Glycosylase Distal Crosslink to Oxog Dna, PDB code: 2nol was solved by A.Banerjee, C.T.Radom, G.L.Verdine, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	50.00 / 2.57
Space group	P 65 2 2
Cell size a, b, c (Å), α, β, γ (°)	92.037, 92.037, 211.368, 90.00, 90.00, 120.00
R / Rfree (%)	20.6 / 25

The binding sites of Calcium atom in the Structure of Catalytically Inactive Human 8-Oxoguanine Glycosylase Distal Crosslink to Oxog Dna (pdb code 2nol). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 2 binding sites of Calcium where determined in the Structure of Catalytically Inactive Human 8-Oxoguanine Glycosylase Distal Crosslink to Oxog Dna, PDB code: 2nol:
Jump to Calcium binding site number: 1; 2;

Calcium binding site 1 out of 2 in the Structure of Catalytically Inactive Human 8-Oxoguanine Glycosylase Distal Crosslink to Oxog Dna


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Structure of Catalytically Inactive Human 8-Oxoguanine Glycosylase Distal Crosslink to Oxog Dna within 5.0Å range: probe atom residue distance (Å) B Occ C:Ca301 b:53.9 occ:1.00 OP2 C:DA22 3.1 33.2 1.0 O A:HOH339 3.8 30.1 1.0 C8 C:DA22 4.0 28.0 1.0 CG2 A:VAL269 4.0 21.3 1.0 N7 C:DA22 4.0 28.6 1.0 N7 C:DG24 4.4 26.7 1.0 OD1 A:ASN149 4.4 24.0 1.0 P C:DA22 4.6 33.5 1.0 OP1 C:DG24 4.7 24.6 1.0 C2' C:DC21 4.7 35.5 1.0

Calcium binding site 2 out of 2 in the Structure of Catalytically Inactive Human 8-Oxoguanine Glycosylase Distal Crosslink to Oxog Dna


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of Structure of Catalytically Inactive Human 8-Oxoguanine Glycosylase Distal Crosslink to Oxog Dna within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca328 b:63.2 occ:1.00 O A:CYS241 2.4 24.7 1.0 O A:HOH456 2.4 31.4 1.0 O A:VAL246 2.6 24.8 1.0 OP2 C:DC26 2.7 29.6 1.0 OP1 C:DC26 3.2 28.8 1.0 O A:LEU243 3.3 22.0 1.0 C A:CYS241 3.4 24.4 1.0 P C:DC26 3.4 27.9 1.0 C A:VAL246 3.6 22.7 1.0 CA A:CYS241 4.0 23.8 1.0 N A:VAL246 4.3 20.6 1.0 O A:ILE242 4.4 20.5 1.0 C A:LEU243 4.4 21.3 1.0 CA A:VAL246 4.4 20.7 1.0 C A:ILE242 4.4 22.1 1.0 N A:ILE242 4.5 24.4 1.0 O3' C:DT25 4.5 26.6 1.0 CB A:CYS241 4.5 24.0 1.0 N A:GLY247 4.5 24.6 1.0 CA A:GLY247 4.7 23.5 1.0 CB A:VAL246 4.7 19.7 1.0 O5' C:DC26 4.7 31.1 1.0 CA A:ILE242 4.8 23.8 1.0 N A:LEU243 4.8 21.6 1.0 O C:HOH167 4.8 31.6 1.0 C3' C:DT25 5.0 23.0 1.0

C.T.Radom, A.Banerjee, G.L.Verdine. Structural Characterization of Human 8-Oxoguanine Dna Glycosylase Variants Bearing Active Site Mutations. J.Biol.Chem. V. 282 9182 2007.
ISSN: ISSN 0021-9258
PubMed: 17114185
DOI: 10.1074/JBC.M608989200