Calcium in PDB 2pc6: Crystal Structure of Putative Acetolactate Synthase- Small Subunit From Nitrosomonas Europaea

All present enzymatic activity of Crystal Structure of Putative Acetolactate Synthase- Small Subunit From Nitrosomonas Europaea:
4.1.3.18;

The structure of Crystal Structure of Putative Acetolactate Synthase- Small Subunit From Nitrosomonas Europaea, PDB code: 2pc6 was solved by J.J.Petkowski, M.Chruszcz, M.D.Zimmerman, H.Zheng, M.T.Cymborowski, T.Skarina, O.Onopriyenko, A.Savchenko, A.Edwards, W.Minor, A.Joachimiak, Midwest Center For Structural Genomics (Mcsg), with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	35.58 / 2.50
Space group	P 42 21 2
Cell size a, b, c (Å), α, β, γ (°)	122.123, 122.123, 111.559, 90.00, 90.00, 90.00
R / Rfree (%)	20.5 / 27.6

The binding sites of Calcium atom in the Crystal Structure of Putative Acetolactate Synthase- Small Subunit From Nitrosomonas Europaea (pdb code 2pc6). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 3 binding sites of Calcium where determined in the Crystal Structure of Putative Acetolactate Synthase- Small Subunit From Nitrosomonas Europaea, PDB code: 2pc6:
Jump to Calcium binding site number: 1; 2; 3;

Calcium binding site 1 out of 3 in the Crystal Structure of Putative Acetolactate Synthase- Small Subunit From Nitrosomonas Europaea


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Crystal Structure of Putative Acetolactate Synthase- Small Subunit From Nitrosomonas Europaea within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca301 b:73.1 occ:1.00 O A:HOH441 2.3 74.6 1.0 O A:ASN65 2.4 51.9 1.0 O A:HOH442 2.5 58.4 1.0 O A:VAL70 2.5 50.9 1.0 O A:LEU67 2.5 49.8 1.0 C A:ASN65 3.5 52.2 1.0 C A:LEU67 3.6 50.5 1.0 C A:VAL70 3.7 51.9 1.0 CA A:ILE68 4.1 51.6 1.0 C A:LYS66 4.3 53.0 1.0 CA A:ASN65 4.3 52.3 1.0 N A:ILE68 4.3 51.2 1.0 C A:ILE68 4.3 51.2 1.0 O A:ILE68 4.4 51.0 1.0 N A:LEU67 4.4 51.7 1.0 O A:LYS66 4.4 53.2 1.0 CB A:VAL70 4.4 51.7 1.0 CA A:VAL70 4.4 51.0 1.0 N A:VAL70 4.5 51.2 1.0 N A:LYS66 4.5 52.4 1.0 CA A:LEU67 4.6 50.6 1.0 CG1 A:VAL70 4.7 50.5 1.0 N A:VAL71 4.7 52.8 1.0 CA A:LYS66 4.7 53.2 1.0 O A:LEU64 4.8 50.6 1.0 ND2 A:ASN65 4.8 53.9 1.0 CA A:VAL71 4.9 53.5 1.0

Calcium binding site 2 out of 3 in the Crystal Structure of Putative Acetolactate Synthase- Small Subunit From Nitrosomonas Europaea


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of Crystal Structure of Putative Acetolactate Synthase- Small Subunit From Nitrosomonas Europaea within 5.0Å range: probe atom residue distance (Å) B Occ B:Ca303 b:62.2 occ:1.00 O B:LEU67 2.4 60.2 1.0 O B:ASN65 2.4 62.0 1.0 O B:VAL70 2.4 57.2 1.0 O B:HOH440 2.5 49.0 1.0 C B:ASN65 3.5 61.6 1.0 C B:LEU67 3.5 60.2 1.0 C B:VAL70 3.6 55.3 1.0 CA B:ASN65 4.0 61.9 1.0 CA B:ILE68 4.3 60.6 1.0 N B:ILE68 4.3 60.3 1.0 CB B:VAL70 4.3 54.7 1.0 O B:LEU64 4.3 60.9 1.0 CA B:VAL70 4.4 55.7 1.0 N B:LEU67 4.4 60.2 1.0 N B:VAL70 4.4 56.9 1.0 C B:ILE68 4.5 60.1 1.0 C B:LYS66 4.5 60.7 1.0 CA B:LEU67 4.5 60.4 1.0 O B:ILE68 4.5 60.0 1.0 N B:VAL71 4.6 54.4 1.0 N B:LYS66 4.7 61.2 1.0 O B:LYS66 4.7 59.9 1.0 CA B:VAL71 4.7 53.4 1.0 CB B:ASN65 4.8 61.9 1.0 OD1 B:ASN65 4.8 65.0 1.0 CG1 B:VAL70 4.8 55.1 1.0

Calcium binding site 3 out of 3 in the Crystal Structure of Putative Acetolactate Synthase- Small Subunit From Nitrosomonas Europaea


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 3 of Crystal Structure of Putative Acetolactate Synthase- Small Subunit From Nitrosomonas Europaea within 5.0Å range: probe atom residue distance (Å) B Occ C:Ca302 b:64.0 occ:1.00 O C:ASN65 2.4 62.0 1.0 O C:LEU67 2.4 60.7 1.0 O C:VAL70 2.4 57.2 1.0 C C:LEU67 3.5 59.1 1.0 C C:ASN65 3.5 61.4 1.0 C C:VAL70 3.6 54.4 1.0 CB C:VAL70 4.1 54.0 1.0 N C:LEU67 4.2 60.6 1.0 N C:ILE68 4.2 57.9 1.0 CA C:ILE68 4.2 56.5 1.0 C C:LYS66 4.3 61.0 1.0 CA C:VAL70 4.3 54.1 1.0 CA C:ASN65 4.4 61.5 1.0 N C:VAL70 4.4 54.1 1.0 CA C:LEU67 4.4 59.7 1.0 N C:LYS66 4.5 61.1 1.0 C C:ILE68 4.5 55.7 1.0 CA C:LYS66 4.6 61.3 1.0 O C:LYS66 4.6 61.0 1.0 O C:ILE68 4.6 55.0 1.0 CG1 C:VAL70 4.6 53.7 1.0 O C:LEU64 4.6 61.2 1.0 N C:VAL71 4.7 53.5 1.0 O C:VAL71 4.7 51.2 1.0 CA C:VAL71 4.9 51.9 1.0 C C:VAL71 5.0 51.3 1.0

J.J.Petkowski, M.Chruszcz, M.D.Zimmerman, H.Zheng, T.Skarina, O.Onopriyenko, M.T.Cymborowski, K.D.Koclega, A.Savchenko, A.Edwards, W.Minor. Crystal Structures of TM0549 and NE1324--Two Orthologs of E. Coli Ahas Isozyme III Small Regulatory Subunit. Protein Sci. V. 16 1360 2007.
ISSN: ISSN 0961-8368
PubMed: 17586771
DOI: 10.1110/PS.072793807