Calcium in PDB 2vcp: Crystal Structure of N-Wasp Vc Domain in Complex with Skeletal Actin

The structure of Crystal Structure of N-Wasp Vc Domain in Complex with Skeletal Actin, PDB code: 2vcp was solved by J.F.Gaucher, D.Didry, M.F.Carlier, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	20.00 / 3.20
Space group	P 61
Cell size a, b, c (Å), α, β, γ (°)	136.240, 136.240, 205.410, 90.00, 90.00, 120.00
R / Rfree (%)	27.5 / 33.1

The binding sites of Calcium atom in the Crystal Structure of N-Wasp Vc Domain in Complex with Skeletal Actin (pdb code 2vcp). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 2 binding sites of Calcium where determined in the Crystal Structure of N-Wasp Vc Domain in Complex with Skeletal Actin, PDB code: 2vcp:
Jump to Calcium binding site number: 1; 2;

Calcium binding site 1 out of 2 in the Crystal Structure of N-Wasp Vc Domain in Complex with Skeletal Actin


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Crystal Structure of N-Wasp Vc Domain in Complex with Skeletal Actin within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca1377 b:7.6 occ:1.00 O2B A:ATP1376 2.9 12.5 1.0 O3G A:ATP1376 3.1 12.5 1.0 O1G A:ATP1376 3.1 12.5 1.0 NE2 A:GLN137 3.6 23.4 1.0 PG A:ATP1376 3.6 12.5 1.0 OD1 A:ASP154 3.7 33.0 1.0 NZ A:LYS18 3.9 33.5 1.0 CD A:GLN137 3.9 23.4 1.0 O A:ASN12 4.0 26.3 1.0 OE1 A:GLN137 4.0 23.4 1.0 PB A:ATP1376 4.1 12.5 1.0 O3B A:ATP1376 4.3 12.5 1.0 OD1 A:ASP11 4.3 11.1 1.0 CA A:GLY13 4.5 26.9 1.0 CG2 A:VAL339 4.7 12.8 1.0 CG A:ASP154 4.7 33.0 1.0 O1A A:ATP1376 4.8 12.5 1.0 CB A:GLN137 4.8 23.4 1.0 CG A:GLN137 4.8 23.4 1.0 C A:ASN12 4.9 26.3 1.0 O3A A:ATP1376 5.0 12.5 1.0 OD2 A:ASP11 5.0 11.1 1.0 OD2 A:ASP154 5.0 33.0 1.0

Calcium binding site 2 out of 2 in the Crystal Structure of N-Wasp Vc Domain in Complex with Skeletal Actin


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of Crystal Structure of N-Wasp Vc Domain in Complex with Skeletal Actin within 5.0Å range: probe atom residue distance (Å) B Occ B:Ca1377 b:11.3 occ:1.00 O2B B:ATP1376 2.8 13.2 1.0 O3G B:ATP1376 3.1 13.2 1.0 O1G B:ATP1376 3.1 13.2 1.0 NE2 B:GLN137 3.2 23.4 1.0 NZ B:LYS18 3.6 33.3 1.0 PG B:ATP1376 3.6 13.2 1.0 O B:ASN12 3.6 25.6 1.0 CD B:GLN137 3.7 23.4 1.0 OD1 B:ASP11 3.8 12.3 1.0 OD1 B:ASP154 4.0 33.5 1.0 OE1 B:GLN137 4.0 23.4 1.0 PB B:ATP1376 4.1 13.2 1.0 CA B:GLY13 4.2 27.1 1.0 O3B B:ATP1376 4.3 13.2 1.0 OD2 B:ASP11 4.3 12.3 1.0 CG B:ASP11 4.5 12.3 1.0 C B:ASN12 4.5 25.6 1.0 CG B:GLN137 4.7 23.4 1.0 CB B:GLN137 4.8 23.4 1.0 N B:GLY13 4.8 27.1 1.0 CG2 B:VAL339 4.8 13.1 1.0 CG B:ASP154 4.9 33.5 1.0 O1A B:ATP1376 4.9 13.2 1.0 CE B:LYS18 5.0 33.3 1.0

J.F.Gaucher, C.Mauge, D.Didry, B.Guichard, L.Renault, M.F.Carlier. Interactions of Isolated C-Terminal Fragments of Neural Wiskott-Aldrich Syndrome Protein (N-Wasp) with Actin and ARP2/3 Complex. J. Biol. Chem. V. 287 34646 2012.
ISSN: ESSN 1083-351X
PubMed: 22847007
DOI: 10.1074/JBC.M112.394361