Calcium in PDB 2w2o: PCSK9-Deltac D374Y Mutant Bound to Wt Egf-A of Ldlr

The structure of PCSK9-Deltac D374Y Mutant Bound to Wt Egf-A of Ldlr, PDB code: 2w2o was solved by M.J.Bottomley, A.Cirillo, L.Orsatti, L.Ruggeri, T.S.Fisher, J.C.Santoro, R.T.Cummings, R.M.Cubbon, P.Lo Surdo, A.Calzetta, A.Noto, J.Baysarowich, M.Mattu, F.Talamo, R.De Francesco, C.P.Sparrow, A.Sitlani, A.Carfi, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	40.00 / 2.62
Space group	P 43 21 2
Cell size a, b, c (Å), α, β, γ (°)	85.948, 85.948, 218.329, 90.00, 90.00, 90.00
R / Rfree (%)	22.659 / 26.311

The binding sites of Calcium atom in the PCSK9-Deltac D374Y Mutant Bound to Wt Egf-A of Ldlr (pdb code 2w2o). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total only one binding site of Calcium was determined in the PCSK9-Deltac D374Y Mutant Bound to Wt Egf-A of Ldlr, PDB code: 2w2o:

Calcium binding site 1 out of 1 in the PCSK9-Deltac D374Y Mutant Bound to Wt Egf-A of Ldlr


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of PCSK9-Deltac D374Y Mutant Bound to Wt Egf-A of Ldlr within 5.0Å range: probe atom residue distance (Å) B Occ E:Ca1333 b:93.3 occ:1.00 O E:THR294 2.4 54.5 1.0 OD2 E:ASP310 2.4 58.2 1.0 OE1 E:GLU296 2.5 59.9 1.0 O E:GLY314 2.5 59.7 1.0 O E:LEU311 2.5 56.2 1.0 C E:GLY314 3.5 60.1 1.0 C E:THR294 3.6 54.6 1.0 CD E:GLU296 3.6 59.0 1.0 CG E:ASP310 3.6 57.3 1.0 C E:LEU311 3.7 56.3 1.0 N E:GLY314 3.8 58.5 1.0 N E:LEU311 3.8 56.3 1.0 CA E:GLY314 3.8 59.3 1.0 OE2 E:GLU296 3.9 60.1 1.0 N E:THR294 4.2 54.0 1.0 OD1 E:ASP310 4.2 58.2 1.0 C E:ASP310 4.3 56.4 1.0 CA E:LEU311 4.3 56.2 1.0 O E:LYS312 4.4 56.8 1.0 CA E:THR294 4.4 54.5 1.0 N E:ASN295 4.5 54.6 1.0 CA E:ASP310 4.5 56.5 1.0 CA E:ASN295 4.5 54.9 1.0 C E:ILE313 4.6 58.0 1.0 C E:LYS312 4.6 56.9 1.0 N E:GLU296 4.6 55.1 1.0 CB E:ASP310 4.6 56.6 1.0 N E:TYR315 4.7 60.8 1.0 N E:LYS312 4.7 56.6 1.0 CG E:GLU296 4.9 57.3 1.0 O E:ALA291 4.9 52.6 1.0 O E:ASP310 5.0 56.8 1.0 CB E:TYR315 5.0 61.3 1.0 CA E:LYS312 5.0 56.9 1.0 CB E:THR294 5.0 54.5 1.0

M.J.Bottomley, A.Cirillo, L.Orsatti, L.Ruggeri, T.S.Fisher, J.C.Santoro, R.T.Cummings, R.M.Cubbon, P.Lo Surdo, A.Calzetta, A.Noto, J.Baysarowich, M.Mattu, F.Talamo, R.De Francesco, C.P.Sparrow, A.Sitlani, A.Carfi. Structural and Biochemical Characterization of the Wild Type PCSK9/Egf-Ab Complex and Natural Fh Mutants. J.Biol.Chem. V. 284 1313 2009.
ISSN: ISSN 0021-9258
PubMed: 19001363
DOI: 10.1074/JBC.M808363200