Calcium in PDB 2w3j: Structure of A Family 35 Carbohydrate Binding Module From An Environmental Isolate

The structure of Structure of A Family 35 Carbohydrate Binding Module From An Environmental Isolate, PDB code: 2w3j was solved by C.Montainer, J.Flint, T.M.Gloster, J.P.Turkenburg, G.J.Davies, H.J.Gilbert, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	53.68 / 1.70
Space group	H 3
Cell size a, b, c (Å), α, β, γ (°)	83.943, 83.943, 79.498, 90.00, 90.00, 120.00
R / Rfree (%)	16.2 / 18.8

The binding sites of Calcium atom in the Structure of A Family 35 Carbohydrate Binding Module From An Environmental Isolate (pdb code 2w3j). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 2 binding sites of Calcium where determined in the Structure of A Family 35 Carbohydrate Binding Module From An Environmental Isolate, PDB code: 2w3j:
Jump to Calcium binding site number: 1; 2;

Calcium binding site 1 out of 2 in the Structure of A Family 35 Carbohydrate Binding Module From An Environmental Isolate


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Structure of A Family 35 Carbohydrate Binding Module From An Environmental Isolate within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca1138 b:29.5 occ:1.00 OD1 A:ASP32 2.3 16.8 1.0 O A:HOH2077 2.4 18.9 1.0 O A:HOH2195 2.4 18.4 1.0 O A:HOH2196 2.4 14.1 1.0 O A:THR33 2.5 16.3 1.0 O A:HOH2197 2.5 20.6 1.0 N A:THR33 3.4 15.2 1.0 CG A:ASP32 3.5 19.1 1.0 C A:THR33 3.5 17.0 1.0 O A:HOH2071 3.7 37.4 1.0 C A:ASP32 3.8 14.5 1.0 OG1 A:THR33 3.8 15.0 1.0 NH1 A:ARG67 3.9 15.6 1.0 OD1 A:ASN35 3.9 24.3 1.0 OD1 A:ASN120 3.9 14.1 1.0 CA A:THR33 4.0 15.4 1.0 O A:LEU118 4.0 14.7 1.0 CA A:ASP32 4.1 14.7 1.0 OD2 A:ASP32 4.3 24.6 1.0 CB A:ASP32 4.4 14.3 1.0 O A:ASP32 4.5 14.8 1.0 CB A:THR33 4.6 16.0 1.0 O A:HOH2067 4.6 41.4 1.0 O A:HOH2068 4.7 23.4 1.0 N A:GLU34 4.7 18.7 1.0 O A:GLU34 4.8 19.7 1.0 O A:HOH2112 4.8 39.6 1.0 C A:GLU34 4.8 20.4 1.0 O A:HOH2014 4.9 25.8 0.5 O A:ASP116 4.9 19.6 1.0 CZ A:ARG67 5.0 17.9 1.0

Calcium binding site 2 out of 2 in the Structure of A Family 35 Carbohydrate Binding Module From An Environmental Isolate


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of Structure of A Family 35 Carbohydrate Binding Module From An Environmental Isolate within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca1139 b:22.8 occ:1.00 OD1 A:ASP122 2.4 14.9 1.0 O A:GLY29 2.4 14.5 1.0 OE1 A:GLU5 2.4 16.2 1.0 O A:THR26 2.4 15.2 1.0 O A:ASP122 2.4 13.4 1.0 OE1 A:GLU6 2.6 24.4 1.0 OE2 A:GLU6 3.3 27.2 1.0 CD A:GLU6 3.3 24.2 1.0 C A:ASP122 3.3 13.3 1.0 CG A:ASP122 3.4 15.4 1.0 C A:THR26 3.4 17.8 1.0 C A:GLY29 3.6 14.0 1.0 CD A:GLU5 3.6 19.2 1.0 CA A:ASP122 3.7 12.1 1.0 OG1 A:THR26 3.9 17.7 1.0 N A:THR26 4.0 16.6 1.0 OE2 A:GLU5 4.2 19.9 1.0 N A:GLY29 4.2 15.4 1.0 CB A:ASP122 4.2 12.4 1.0 CA A:THR26 4.2 16.8 1.0 OD2 A:ASP122 4.3 19.7 1.0 N A:GLY27 4.3 17.9 1.0 O A:HOH2009 4.4 18.2 1.0 CA A:PHE30 4.4 13.4 1.0 CA A:GLY27 4.4 18.8 1.0 N A:PHE30 4.4 13.6 1.0 CA A:GLY29 4.5 13.8 1.0 N A:SER123 4.5 13.5 1.0 CB A:TYR25 4.5 14.1 1.0 CB A:THR26 4.7 17.6 1.0 CB A:GLU5 4.7 17.2 1.0 CB A:SER123 4.7 11.8 1.0 CG A:GLU5 4.7 16.4 1.0 C A:GLY27 4.8 18.8 1.0 CG A:GLU6 4.8 20.4 1.0 N A:THR28 4.8 18.9 1.0 N A:GLU6 4.8 17.8 1.0 CA A:GLU5 4.9 17.1 1.0 CB A:PHE30 4.9 13.2 1.0 CA A:SER123 4.9 12.8 1.0 C A:TYR25 5.0 16.4 1.0

C.Montanier, A.L.Van Bueren, C.Dumon, J.E.Flint, M.A.Correia, J.A.Prates, S.J.Firbank, R.J.Lewis, G.G.Grondin, M.G.Ghinet, T.M.Gloster, C.Herve, J.P.Knox, B.G.Talbot, J.P.Turkenburg, J.Kerovuo, R.Brzezinski, C.M.G.A.Fontes, G.J.Davies, A.B.Boraston, H.J.Gilbert. Evidence That Family 35 Carbohydrate Binding Modules Display Conserved Specificity But Divergent Function. Proc.Natl.Acad.Sci.Usa V. 106 3065 2009.
ISSN: ISSN 0027-8424
PubMed: 19218457
DOI: 10.1073/PNAS.0808972106