Calcium in PDB 2wpi: Factor Ixa Superactive Double Mutant

All present enzymatic activity of Factor Ixa Superactive Double Mutant:
3.4.21.22;

The structure of Factor Ixa Superactive Double Mutant, PDB code: 2wpi was solved by T.Zogg, H.Brandstetter, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	29.56 / 1.99
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	44.320, 67.350, 98.260, 90.00, 90.00, 90.00
R / Rfree (%)	20.5 / 25.2

The binding sites of Calcium atom in the Factor Ixa Superactive Double Mutant (pdb code 2wpi). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total only one binding site of Calcium was determined in the Factor Ixa Superactive Double Mutant, PDB code: 2wpi:

Calcium binding site 1 out of 1 in the Factor Ixa Superactive Double Mutant


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Factor Ixa Superactive Double Mutant within 5.0Å range: probe atom residue distance (Å) B Occ S:Ca1246 b:16.8 occ:1.00 OE1 S:GLU70 2.5 17.1 1.0 O S:ASN72 2.5 11.2 1.0 O S:GLU75 2.5 14.4 1.0 OE2 S:GLU80 2.5 15.6 1.0 OE1 S:GLU77 2.6 17.7 1.0 O S:HOH2045 2.6 16.8 1.0 OE2 S:GLU77 2.8 17.7 1.0 CD S:GLU77 3.0 19.1 1.0 CD S:GLU70 3.4 20.6 1.0 C S:ASN72 3.4 14.7 1.0 C S:GLU75 3.5 16.1 1.0 CD S:GLU80 3.6 11.7 1.0 OE2 S:GLU70 3.7 17.8 1.0 N S:ASN72 3.8 14.1 1.0 CG S:GLU80 4.0 14.8 1.0 CA S:ASN72 4.2 14.7 1.0 N S:GLU77 4.2 15.4 1.0 CA S:THR76 4.2 17.1 1.0 N S:THR76 4.3 16.3 1.0 N S:GLU75 4.3 19.5 1.0 CG S:GLU77 4.4 15.1 1.0 N S:HIS71 4.4 13.8 1.0 N S:ILE73 4.4 17.3 1.0 CA S:GLU75 4.5 20.7 1.0 CB S:ASN72 4.5 13.7 1.0 O S:HOH2066 4.5 39.6 1.0 C S:THR76 4.6 17.8 1.0 CA S:ILE73 4.6 21.0 1.0 C S:ILE73 4.7 22.6 1.0 OE1 S:GLU80 4.7 11.0 1.0 CG S:GLU70 4.8 12.2 1.0 CB S:GLU77 4.8 15.9 1.0 O S:ILE73 4.8 20.2 1.0 C S:HIS71 4.9 11.8 1.0

T.Zogg, H.Brandstetter. Structural Basis of the Cofactor- and Substrate-Assisted Activation of Human Coagulation Factor Ixa Structure V. 17 1669 2009.
ISSN: ISSN 0969-2126
PubMed: 20004170
DOI: 10.1016/J.STR.2009.10.011