Calcium in PDB 2x51: M6 Delta INSERT1

The structure of M6 Delta INSERT1, PDB code: 2x51 was solved by G.Squires, A.Houdusse, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	26.818 / 2.20
Space group	C 1 2 1
Cell size a, b, c (Å), α, β, γ (°)	163.888, 61.017, 133.274, 90.00, 116.19, 90.00
R / Rfree (%)	21.58 / 27.23

The binding sites of Calcium atom in the M6 Delta INSERT1 (pdb code 2x51). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 3 binding sites of Calcium where determined in the M6 Delta INSERT1, PDB code: 2x51:
Jump to Calcium binding site number: 1; 2; 3;

Calcium binding site 1 out of 3 in the M6 Delta INSERT1


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of M6 Delta INSERT1 within 5.0Å range: probe atom residue distance (Å) B Occ B:Ca1150 b:74.7 occ:1.00 OD1 B:ASP23 2.9 61.8 1.0 OD1 B:ASP21 3.4 43.3 1.0 CG B:ASP23 3.6 60.5 1.0 N B:LYS22 3.8 40.3 1.0 CA B:ASP21 3.8 40.6 1.0 O B:THR27 3.9 41.3 1.0 OD2 B:ASP23 3.9 63.5 1.0 CG B:GLU32 3.9 44.1 1.0 CD B:GLU32 4.0 48.9 1.0 C B:ASP21 4.0 40.6 1.0 O B:PHE20 4.0 40.0 1.0 N B:ASP23 4.1 46.9 1.0 OE2 B:GLU32 4.1 58.6 1.0 CD1 B:ILE28 4.2 46.0 1.0 OD2 B:ASP25 4.3 61.4 1.0 CG B:ASP21 4.4 42.1 1.0 OE1 B:GLU32 4.4 44.8 1.0 CB B:ASP21 4.6 36.3 1.0 CA B:LYS22 4.8 45.2 1.0 CB B:ASP23 4.8 58.5 1.0 O B:ASP21 4.8 42.2 1.0 OD1 B:ASP25 4.8 57.5 1.0 N B:ASP21 4.8 37.7 1.0 C B:PHE20 4.8 39.0 1.0 CG B:ASP25 4.9 58.4 1.0 CA B:ASP23 5.0 54.1 1.0 C B:LYS22 5.0 48.4 1.0

Calcium binding site 2 out of 3 in the M6 Delta INSERT1


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of M6 Delta INSERT1 within 5.0Å range: probe atom residue distance (Å) B Occ B:Ca1151 b:40.0 occ:1.00 OD1 B:ASP94 2.4 35.4 1.0 O B:PHE100 2.4 38.8 1.0 OD1 B:ASN98 2.5 42.1 1.0 OE2 B:GLU105 2.5 44.1 1.0 OE1 B:GLU105 2.6 39.0 1.0 OD1 B:ASP96 2.7 40.3 1.0 CD B:GLU105 2.9 43.1 1.0 CG B:ASP96 3.4 42.0 1.0 CG B:ASN98 3.5 49.0 1.0 CG B:ASP94 3.5 40.9 1.0 C B:PHE100 3.6 41.5 1.0 OD2 B:ASP96 3.7 45.8 1.0 ND2 B:ASN98 4.0 39.0 1.0 CA B:ASP94 4.2 38.1 1.0 N B:PHE100 4.3 40.0 1.0 CB B:ASP94 4.3 37.0 1.0 N B:ASP96 4.3 41.8 1.0 CG B:GLU105 4.4 35.0 1.0 N B:ASN98 4.4 43.0 1.0 OD2 B:ASP94 4.4 37.8 1.0 CA B:ILE101 4.4 41.2 1.0 C B:ASP94 4.5 38.8 1.0 N B:ILE101 4.5 42.0 1.0 CA B:PHE100 4.5 42.4 1.0 NE2 B:GLN136 4.6 48.9 1.0 CB B:ASP96 4.6 39.1 1.0 N B:SER102 4.6 36.4 1.0 N B:GLY97 4.6 41.8 1.0 N B:LYS95 4.6 35.8 1.0 CB B:ASN98 4.7 42.8 1.0 CA B:ASP96 4.8 42.6 1.0 N B:GLY99 4.9 37.5 1.0 C B:ASP96 4.9 41.8 1.0 O B:HOH2044 4.9 43.0 1.0 CA B:ASN98 5.0 43.2 1.0 CB B:PHE100 5.0 45.7 1.0 C B:ILE101 5.0 39.5 1.0

Calcium binding site 3 out of 3 in the M6 Delta INSERT1


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 3 of M6 Delta INSERT1 within 5.0Å range: probe atom residue distance (Å) B Occ B:Ca1152 b:47.0 occ:1.00 O B:GLN136 2.3 45.9 1.0 OD1 B:ASP130 2.5 47.0 1.0 OE2 B:GLU141 2.6 53.3 1.0 O B:HOH2050 2.6 50.2 1.0 OE1 B:GLU141 2.7 45.1 1.0 OD1 B:ASP132 2.8 46.0 1.0 OD1 B:ASP134 2.9 51.8 1.0 CD B:GLU141 3.0 50.1 1.0 CG B:ASP134 3.4 52.8 1.0 CG B:ASP130 3.4 48.1 1.0 C B:GLN136 3.5 43.3 1.0 CG B:ASP132 3.5 49.9 1.0 OD2 B:ASP134 3.6 55.7 1.0 OD2 B:ASP132 3.7 53.0 1.0 CA B:ASP130 4.1 46.1 1.0 N B:GLN136 4.1 37.4 1.0 CB B:ASP130 4.2 39.0 1.0 OD2 B:ASP130 4.2 39.5 1.0 N B:ASP134 4.2 47.2 1.0 CA B:VAL137 4.3 43.3 1.0 N B:ASN138 4.3 45.0 1.0 N B:VAL137 4.4 41.4 1.0 CA B:GLN136 4.4 43.8 1.0 N B:ASP132 4.5 52.9 1.0 C B:ASP130 4.5 50.8 1.0 CG B:GLU141 4.5 47.0 1.0 N B:GLY133 4.5 52.2 1.0 CB B:ASP134 4.6 44.9 1.0 CB B:ASP132 4.7 52.8 1.0 CG B:ASN138 4.7 47.8 1.0 N B:GLY135 4.7 42.0 1.0 ND2 B:ASN138 4.7 49.1 1.0 N B:ILE131 4.7 50.4 1.0 OD1 B:ASN138 4.8 43.0 1.0 C B:VAL137 4.8 41.8 1.0 CA B:ASP134 4.8 51.1 1.0 C B:ASP132 4.8 49.7 1.0 CA B:ASP132 4.9 52.4 1.0

O.Pylypenko, L.Song, G.Squires, X.Liu, A.B.Zong, A.Houdusse, H.L.Sweeney. Role of Insert-1 of Myosin VI in Modulating Nucleotide Affinity. J.Biol.Chem. V. 286 11716 2011.
ISSN: ISSN 0021-9258
PubMed: 21278381
DOI: 10.1074/JBC.M110.200626