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Calcium in PDB 2xfg: Reassembly and Co-Crystallization of A Family 9 Processive Endoglucanase From Separately Expressed GH9 and CBM3C Modules

Enzymatic activity of Reassembly and Co-Crystallization of A Family 9 Processive Endoglucanase From Separately Expressed GH9 and CBM3C Modules

All present enzymatic activity of Reassembly and Co-Crystallization of A Family 9 Processive Endoglucanase From Separately Expressed GH9 and CBM3C Modules:
3.2.1.4;

Protein crystallography data

The structure of Reassembly and Co-Crystallization of A Family 9 Processive Endoglucanase From Separately Expressed GH9 and CBM3C Modules, PDB code: 2xfg was solved by S.Petkun, R.Lamed, S.Jindou, T.Burstein, O.Yaniv, Y.Shoham, J.W.L.Shimon, E.A.Bayer, F.Frolow, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 20.868 / 1.68
Space group P 21 21 21
Cell size a, b, c (Å), α, β, γ (°) 70.399, 88.541, 106.486, 90.00, 90.00, 90.00
R / Rfree (%) 14.14 / 17.59

Other elements in 2xfg:

The structure of Reassembly and Co-Crystallization of A Family 9 Processive Endoglucanase From Separately Expressed GH9 and CBM3C Modules also contains other interesting chemical elements:

Chlorine (Cl) 3 atoms

Calcium Binding Sites:

The binding sites of Calcium atom in the Reassembly and Co-Crystallization of A Family 9 Processive Endoglucanase From Separately Expressed GH9 and CBM3C Modules (pdb code 2xfg). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 2 binding sites of Calcium where determined in the Reassembly and Co-Crystallization of A Family 9 Processive Endoglucanase From Separately Expressed GH9 and CBM3C Modules, PDB code: 2xfg:
Jump to Calcium binding site number: 1; 2;

Calcium binding site 1 out of 2 in 2xfg

Go back to Calcium Binding Sites List in 2xfg
Calcium binding site 1 out of 2 in the Reassembly and Co-Crystallization of A Family 9 Processive Endoglucanase From Separately Expressed GH9 and CBM3C Modules


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Reassembly and Co-Crystallization of A Family 9 Processive Endoglucanase From Separately Expressed GH9 and CBM3C Modules within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Ca1447

b:15.5
occ:1.00
 O A:ASP261 2.4 15.8 1.0
O A:GLY211 2.4 16.9 1.0
O A:HOH2470 2.5 15.7 1.0
OD1 A:ASP214 2.5 14.3 1.0
OD2 A:ASP214 2.5 14.3 1.0
OE1 A:GLU215 2.5 15.2 1.0
OE2 A:GLU215 2.5 14.4 1.0
OG A:SER210 2.6 16.6 1.0
CG A:ASP214 2.8 17.2 1.0
CD A:GLU215 2.8 15.2 1.0
HA A:ASP262 3.4 13.8 1.0
C A:ASP261 3.6 14.0 1.0
C A:GLY211 3.7 20.4 1.0
CB A:SER210 3.8 20.1 1.0
HG21 A:THR152 3.9 18.6 1.0
HA A:PHE212 3.9 19.1 1.0
HB2 A:SER210 4.0 24.1 1.0
H A:GLU215 4.0 16.8 1.0
N A:GLY211 4.1 20.6 1.0
H A:GLY211 4.1 24.6 1.0
C A:SER210 4.2 20.4 1.0
H A:SER210 4.3 18.2 1.0
CA A:ASP262 4.3 11.5 1.0
H A:VAL263 4.3 16.2 1.0
CG A:GLU215 4.3 11.6 1.0
CB A:ASP214 4.3 14.6 1.0
N A:ASP262 4.3 13.9 1.0
H A:ASP214 4.4 17.3 1.0
HA A:ASP261 4.4 16.2 1.0
CA A:SER210 4.5 16.6 1.0
HG22 A:THR152 4.5 18.6 1.0
HB3 A:SER210 4.5 24.1 1.0
OD2 A:ASP261 4.5 16.8 1.0
CA A:GLY211 4.5 20.9 1.0
OD1 A:ASP261 4.6 16.0 1.0
O A:SER210 4.6 20.4 1.0
N A:PHE212 4.6 18.7 1.0
CG A:ASP261 4.6 18.1 1.0
CA A:PHE212 4.6 16.0 1.0
O A:HOH2411 4.6 24.2 1.0
CA A:ASP261 4.6 13.5 1.0
CG2 A:THR152 4.6 15.5 1.0
HG3 A:GLU215 4.7 13.8 1.0
N A:GLU215 4.7 14.0 1.0
HB2 A:ASP214 4.7 17.5 1.0
HG21 A:VAL263 4.7 15.4 1.0
N A:SER210 4.7 15.2 1.0
OG A:SER208 4.8 18.2 1.0
HB3 A:ASP214 4.8 17.5 1.0
HG2 A:GLU215 4.8 13.8 1.0
HB2 A:GLU215 4.8 16.1 1.0
HG A:SER208 4.9 21.8 1.0
N A:ASP214 5.0 14.5 1.0

Calcium binding site 2 out of 2 in 2xfg

Go back to Calcium Binding Sites List in 2xfg
Calcium binding site 2 out of 2 in the Reassembly and Co-Crystallization of A Family 9 Processive Endoglucanase From Separately Expressed GH9 and CBM3C Modules


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of Reassembly and Co-Crystallization of A Family 9 Processive Endoglucanase From Separately Expressed GH9 and CBM3C Modules within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Ca1619

b:14.0
occ:1.00
 OD1 B:ASN581 2.3 14.0 1.0
O B:ASN505 2.4 14.3 1.0
O B:ASN578 2.4 14.1 1.0
OD1 B:ASP582 2.5 12.6 1.0
O B:HOH2235 2.5 15.0 1.0
OE2 B:GLU508 2.5 17.0 1.0
OE1 B:GLU508 2.7 16.4 1.0
CD B:GLU508 3.0 16.2 1.0
CG B:ASN581 3.4 16.0 1.0
CG B:ASP582 3.4 13.6 1.0
HD22 B:ASN581 3.5 25.3 1.0
H B:ASN505 3.5 13.2 1.0
C B:ASN578 3.5 18.1 1.0
C B:ASN505 3.6 14.5 1.0
HB3 B:ASN505 3.6 16.2 1.0
HB3 B:ASN578 3.6 16.7 1.0
H B:ASN578 3.7 17.6 1.0
OD2 B:ASP582 3.7 13.4 1.0
ND2 B:ASN581 3.8 21.1 1.0
HA B:ILE506 4.0 14.7 1.0
HA B:PRO579 4.1 17.3 1.0
H B:ASN581 4.1 16.1 1.0
N B:ASN505 4.2 11.0 1.0
CA B:ASN578 4.3 12.5 1.0
N B:ASN578 4.3 14.7 1.0
CA B:ASN505 4.3 10.4 1.0
CB B:ASN578 4.3 14.0 1.0
CB B:ASN505 4.4 13.5 1.0
HB B:ILE504 4.4 12.3 1.0
CG B:GLU508 4.5 13.8 1.0
N B:PRO579 4.5 14.5 1.0
N B:ASP582 4.6 13.6 1.0
H B:GLU508 4.6 22.2 1.0
N B:ILE506 4.6 13.5 1.0
H B:ASP582 4.6 16.3 1.0
CA B:PRO579 4.6 14.4 1.0
C B:ASN581 4.7 12.9 1.0
HA B:ASP582 4.7 15.8 1.0
HD21 B:ASN581 4.7 25.3 1.0
CA B:ILE506 4.7 12.3 1.0
HG3 B:GLU508 4.7 16.5 1.0
CB B:ASN581 4.7 17.9 1.0
CB B:ASP582 4.7 14.2 1.0
N B:ASN581 4.8 13.4 1.0
HG2 B:GLU508 4.9 16.5 1.0
CG B:ASN578 4.9 21.4 1.0
C B:PRO579 4.9 15.9 1.0
CA B:ASP582 4.9 13.2 1.0
CA B:ASN581 5.0 16.7 1.0
H B:GLU507 5.0 16.7 1.0

Reference:

S.Petkun, I.R.Grinberg, R.Lamed, S.Jindou, T.Burstein, O.Yaniv, Y.Shoham, J.W.L.Shimon, E.A.Bayer, F.Frolow. Reassembly and Co-Crystallization of A Family 9 Processive Endoglucanase From Its Component Parts: Structural and Functional Significance of Intermodular Linker Peerj V. 3 E1126 2015.
ISSN: ISSN 2167-8359
PubMed: 26401442
DOI: 10.7717/PEERJ.1126
Page generated: Fri Jul 12 19:05:03 2024

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