Calcium in PDB 2xsg: Structure of the GH92 Family Glycosyl Hydrolase CCMAN5

All present enzymatic activity of Structure of the GH92 Family Glycosyl Hydrolase CCMAN5:
3.2.1.24;

The structure of Structure of the GH92 Family Glycosyl Hydrolase CCMAN5, PDB code: 2xsg was solved by E.Baranova, P.Tiels, H.Remaut, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	47.80 / 2.00
Space group	P 2 2 21
Cell size a, b, c (Å), α, β, γ (°)	82.202, 91.158, 224.523, 90.00, 90.00, 90.00
R / Rfree (%)	19.067 / 23.861

The binding sites of Calcium atom in the Structure of the GH92 Family Glycosyl Hydrolase CCMAN5 (pdb code 2xsg). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 2 binding sites of Calcium where determined in the Structure of the GH92 Family Glycosyl Hydrolase CCMAN5, PDB code: 2xsg:
Jump to Calcium binding site number: 1; 2;

Calcium binding site 1 out of 2 in the Structure of the GH92 Family Glycosyl Hydrolase CCMAN5


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Structure of the GH92 Family Glycosyl Hydrolase CCMAN5 within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca1772 b:30.1 occ:1.00 OD1 A:ASN588 2.4 9.8 1.0 O A:HOH2338 2.4 7.5 1.0 OD2 A:ASP662 2.5 3.8 1.0 OE1 A:GLU589 2.6 5.0 1.0 O A:HOH2370 3.4 26.4 1.0 CD A:GLU589 3.5 6.2 1.0 CG A:ASP662 3.5 4.7 1.0 OE2 A:GLU589 3.6 6.1 1.0 CG A:ASN588 3.6 8.5 1.0 OD1 A:ASP662 3.8 5.7 1.0 O A:HOH2218 3.8 15.0 1.0 NE2 A:GLN536 4.0 11.9 1.0 O A:HOH2310 4.3 13.1 1.0 OD2 A:ASP355 4.3 7.2 1.0 CA A:ASN588 4.4 7.0 1.0 ND2 A:ASN588 4.5 10.5 1.0 O A:ASP660 4.5 3.8 1.0 CB A:ASN588 4.5 7.1 1.0 N A:GLU589 4.6 6.9 1.0 O A:ALA587 4.7 6.0 1.0 OE1 A:GLN536 4.8 11.4 1.0 CD A:GLN536 4.8 12.3 1.0 CB A:ASP662 4.8 3.3 1.0 CB A:ASP660 4.9 3.9 1.0 CG A:GLU589 4.9 6.9 1.0 CG A:ASP355 4.9 4.7 1.0 CB A:ALA625 5.0 5.4 1.0

Calcium binding site 2 out of 2 in the Structure of the GH92 Family Glycosyl Hydrolase CCMAN5


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of Structure of the GH92 Family Glycosyl Hydrolase CCMAN5 within 5.0Å range: probe atom residue distance (Å) B Occ B:Ca1772 b:32.3 occ:1.00 OD1 B:ASN588 2.3 15.2 1.0 O B:HOH2366 2.4 3.5 1.0 O B:HOH2367 2.5 23.6 1.0 OE1 B:GLU589 2.5 10.4 1.0 OD2 B:ASP662 2.5 5.4 1.0 O B:HOH2247 2.6 23.1 1.0 CD B:GLU589 3.4 10.7 1.0 CG B:ASN588 3.4 12.3 1.0 CG B:ASP662 3.6 5.8 1.0 OE2 B:GLU589 3.6 8.0 1.0 O B:HOH2368 4.0 20.8 1.0 OD1 B:ASP662 4.0 7.2 1.0 OD2 B:ASP355 4.1 15.0 1.0 CA B:ASN588 4.2 9.7 1.0 ND2 B:ASN588 4.3 15.0 1.0 O B:ASP660 4.3 4.7 1.0 CB B:ASN588 4.4 10.1 1.0 N B:GLU589 4.5 10.9 1.0 OE1 B:GLN536 4.5 18.0 1.0 O B:ALA587 4.6 6.5 1.0 CB B:ASP660 4.7 5.0 1.0 CB B:ASP662 4.8 5.0 1.0 CG B:GLU589 4.8 10.0 1.0 CG B:ASP355 4.8 11.2 1.0 C B:ASN588 4.9 10.2 1.0 CB B:ALA625 4.9 8.7 1.0

P.Tiels, E.Baranova, K.Piens, C.De Visscher, G.Pynaert, W.Nerinckx, J.Stout, F.Fudalej, P.Hulpiau, S.Tannler, S.Geysens, A.Van Hecke, A.Valevska, W.Vervecken, H.Remaut, N.Callewaert. A Bacterial Glycosidase Enables Mannose-6-Phosphate Modification and Improved Cellular Uptake of Yeast-Produced Recombinant Human Lysosomal Enzymes. Nat.Biotechnol. V. 30 1225 2012.
ISSN: ISSN 1087-0156
PubMed: 23159880
DOI: 10.1038/NBT.2427