Calcium in PDB 2y09: The Cyanobacterial PP2C-Like Phosphatase Tppha Requires Three Metals in the Catalytic Center For Efficient Catalysis

The structure of The Cyanobacterial PP2C-Like Phosphatase Tppha Requires Three Metals in the Catalytic Center For Efficient Catalysis, PDB code: 2y09 was solved by C.Schlicker, S.Jiyong, K.Forchhammer, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	36.330 / 1.70
Space group	C 2 2 21
Cell size a, b, c (Å), α, β, γ (°)	38.714, 151.678, 82.777, 90.00, 90.00, 90.00
R / Rfree (%)	19.23 / 21.85

The structure of The Cyanobacterial PP2C-Like Phosphatase Tppha Requires Three Metals in the Catalytic Center For Efficient Catalysis also contains other interesting chemical elements:

Magnesium

(Mg)

2 atoms

The binding sites of Calcium atom in the The Cyanobacterial PP2C-Like Phosphatase Tppha Requires Three Metals in the Catalytic Center For Efficient Catalysis (pdb code 2y09). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 2 binding sites of Calcium where determined in the The Cyanobacterial PP2C-Like Phosphatase Tppha Requires Three Metals in the Catalytic Center For Efficient Catalysis, PDB code: 2y09:
Jump to Calcium binding site number: 1; 2;

Calcium binding site 1 out of 2 in the The Cyanobacterial PP2C-Like Phosphatase Tppha Requires Three Metals in the Catalytic Center For Efficient Catalysis


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of The Cyanobacterial PP2C-Like Phosphatase Tppha Requires Three Metals in the Catalytic Center For Efficient Catalysis within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca1241 b:15.1 occ:1.00 O A:GLY35 2.3 16.0 1.0 O A:HOH2018 2.4 20.8 1.0 OD1 A:ASP34 2.4 13.8 1.0 O A:HOH2110 2.4 14.3 1.0 O A:HOH2108 2.5 18.5 1.0 O A:HOH2037 2.6 31.5 1.0 O A:HOH2034 2.7 28.7 1.0 C A:GLY35 3.5 20.6 1.0 CG A:ASP34 3.6 14.9 1.0 CA A:CA1242 3.9 15.5 1.0 N A:GLY35 4.1 12.1 1.0 OD2 A:ASP34 4.2 14.9 1.0 C A:ASP34 4.3 12.6 1.0 O A:HOH2036 4.3 27.4 1.0 CA A:GLY35 4.4 14.6 1.0 OE1 A:GLN17 4.4 24.1 1.0 O A:HOH2111 4.4 12.1 1.0 N A:MET36 4.4 19.7 1.0 O A:HOH2014 4.4 28.3 1.0 OD1 A:ASP18 4.4 13.1 1.0 CB A:GLN17 4.4 17.9 1.0 CA A:MET36 4.5 25.2 1.0 O A:ASP34 4.6 13.7 1.0 ND2 A:ASN232 4.6 12.0 1.0 CB A:ASP34 4.7 12.2 1.0 CB A:MET36 4.8 24.2 1.0 CA A:ASP34 4.8 10.9 1.0 OD1 A:ASP231 4.8 15.8 1.0 O A:HOH2112 4.8 23.6 1.0 O A:HOH2085 4.9 27.6 1.0

Calcium binding site 2 out of 2 in the The Cyanobacterial PP2C-Like Phosphatase Tppha Requires Three Metals in the Catalytic Center For Efficient Catalysis


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of The Cyanobacterial PP2C-Like Phosphatase Tppha Requires Three Metals in the Catalytic Center For Efficient Catalysis within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca1242 b:15.5 occ:1.00 OD1 A:ASP193 2.3 13.9 1.0 OD2 A:ASP34 2.4 14.9 1.0 OD2 A:ASP231 2.4 17.7 1.0 O A:HOH2111 2.5 12.1 1.0 O A:HOH2108 2.5 18.5 1.0 O A:HOH2085 2.5 27.6 1.0 O A:HOH2034 3.1 28.7 1.0 CG A:ASP34 3.2 14.9 1.0 OD1 A:ASP34 3.2 13.8 1.0 CG A:ASP193 3.4 20.2 1.0 CG A:ASP231 3.4 17.7 1.0 OD2 A:ASP193 3.7 22.0 1.0 OD1 A:ASP231 3.7 15.8 1.0 CA A:CA1241 3.9 15.1 1.0 O A:HOH2109 4.1 20.9 1.0 O A:HOH2035 4.3 11.6 1.0 O A:HOH2014 4.3 28.3 1.0 N A:GLY194 4.4 13.8 1.0 O A:HOH2015 4.4 28.7 1.0 O A:HOH2110 4.5 14.3 1.0 CB A:ASP34 4.6 12.2 1.0 N A:ASP193 4.6 12.7 1.0 OD1 A:ASP18 4.7 13.1 1.0 CB A:ASP193 4.7 13.5 1.0 O A:ASN232 4.7 12.8 1.0 CB A:ASP231 4.7 16.3 1.0 C A:ASP193 4.7 14.5 1.0 CA A:ASP193 4.9 15.2 1.0 CA A:GLY194 5.0 13.1 1.0 O A:HOH2086 5.0 18.9 1.0

J.Su, C.Schlicker, K.Forchhammer. A Third Metal Is Required For Catalytic Activity of the Signal-Transducing Protein Phosphatase M Tppha. J.Biol.Chem. V. 286 13481 2011.
ISSN: ISSN 0021-9258
PubMed: 21310952
DOI: 10.1074/JBC.M109.036467