Calcium in PDB 2zwo: Crystal Structure of CA2 Site Mutant of Pro-S324A
Enzymatic activity of Crystal Structure of CA2 Site Mutant of Pro-S324A
All present enzymatic activity of Crystal Structure of CA2 Site Mutant of Pro-S324A:
3.4.21.62;
Protein crystallography data
The structure of Crystal Structure of CA2 Site Mutant of Pro-S324A, PDB code: 2zwo
was solved by
Y.Takeuchi,
S.Tanaka,
H.Matsumura,
Y.Koga,
K.Takano,
S.Kanaya,
with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:
|
Resolution Low / High (Å)
|
46.63 /
2.07
|
|
Space group
|
P 21 21 21
|
|
Cell size a, b, c (Å), α, β, γ (°)
|
93.729,
118.314,
120.633,
90.00,
90.00,
90.00
|
|
R / Rfree (%)
|
18.4 /
22.7
|
Calcium Binding Sites:
Pages:
>>> Page 1 <<<
Page 2, Binding sites: 11 -
16;
Binding sites:
The binding sites of Calcium atom in the Crystal Structure of CA2 Site Mutant of Pro-S324A
(pdb code 2zwo). This binding sites where shown within
5.0 Angstroms radius around Calcium atom.
In total 16 binding sites of Calcium where determined in the
Crystal Structure of CA2 Site Mutant of Pro-S324A, PDB code: 2zwo:
Jump to Calcium binding site number:
1;
2;
3;
4;
5;
6;
7;
8;
9;
10;
Calcium binding site 1 out
of 16 in 2zwo
Go back to
Calcium Binding Sites List in 2zwo
Calcium binding site 1 out
of 16 in the Crystal Structure of CA2 Site Mutant of Pro-S324A
 Mono view
 Stereo pair view
|
|
A full contact list of Calcium with other atoms in the Ca binding
site number 1 of Crystal Structure of CA2 Site Mutant of Pro-S324A within 5.0Å range:
|
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Ca399
b:24.8
occ:1.00
|
O
|
A:LEU164
|
2.2
|
22.7
|
1.0
|
|
OD1
|
A:ASN166
|
2.3
|
23.5
|
1.0
|
|
O
|
A:VAL170
|
2.3
|
25.1
|
1.0
|
|
O
|
A:ILE168
|
2.4
|
22.7
|
1.0
|
|
OD1
|
A:ASP124
|
2.4
|
26.2
|
1.0
|
|
OD2
|
A:ASP124
|
2.6
|
19.4
|
1.0
|
|
OE1
|
A:GLN84
|
2.6
|
30.1
|
1.0
|
|
CG
|
A:ASP124
|
2.9
|
22.6
|
1.0
|
|
CG
|
A:ASN166
|
3.4
|
21.9
|
1.0
|
|
C
|
A:LEU164
|
3.4
|
28.1
|
1.0
|
|
C
|
A:VAL170
|
3.5
|
24.2
|
1.0
|
|
CD
|
A:GLN84
|
3.6
|
23.1
|
1.0
|
|
C
|
A:ILE168
|
3.6
|
25.9
|
1.0
|
|
N
|
A:VAL170
|
3.7
|
21.8
|
1.0
|
|
N
|
A:ASN166
|
3.8
|
24.8
|
1.0
|
|
ND2
|
A:ASN166
|
3.9
|
20.6
|
1.0
|
|
CG
|
A:GLN84
|
3.9
|
21.4
|
1.0
|
|
C
|
A:GLY169
|
4.1
|
25.0
|
1.0
|
|
CA
|
A:VAL170
|
4.2
|
18.8
|
1.0
|
|
CG1
|
A:ILE168
|
4.3
|
28.3
|
1.0
|
|
CA
|
A:LEU164
|
4.3
|
27.5
|
1.0
|
|
N
|
A:ILE168
|
4.3
|
25.6
|
1.0
|
|
CB
|
A:ASP124
|
4.4
|
23.0
|
1.0
|
|
N
|
A:ASN165
|
4.4
|
24.6
|
1.0
|
|
N
|
A:LEU164
|
4.4
|
28.3
|
1.0
|
|
CA
|
A:GLY169
|
4.5
|
22.5
|
1.0
|
|
N
|
A:GLY169
|
4.5
|
21.4
|
1.0
|
|
CA
|
A:ILE168
|
4.5
|
22.9
|
1.0
|
|
N
|
A:VAL171
|
4.5
|
23.2
|
1.0
|
|
CA
|
A:ASN165
|
4.5
|
24.9
|
1.0
|
|
CA
|
A:ASN166
|
4.5
|
22.6
|
1.0
|
|
C
|
A:ASN165
|
4.6
|
25.9
|
1.0
|
|
CB
|
A:ASN166
|
4.6
|
20.8
|
1.0
|
|
CB
|
A:GLN84
|
4.6
|
28.4
|
1.0
|
|
CB
|
A:LEU164
|
4.6
|
26.4
|
1.0
|
|
CA
|
A:VAL171
|
4.6
|
22.1
|
1.0
|
|
C
|
A:ASN166
|
4.6
|
27.6
|
1.0
|
|
NE2
|
A:GLN84
|
4.8
|
22.1
|
1.0
|
|
O
|
A:ASN166
|
4.8
|
27.6
|
1.0
|
|
O
|
A:GLY169
|
4.8
|
23.4
|
1.0
|
|
CB
|
A:VAL171
|
4.8
|
26.7
|
1.0
|
|
Calcium binding site 2 out
of 16 in 2zwo
Go back to
Calcium Binding Sites List in 2zwo
Calcium binding site 2 out
of 16 in the Crystal Structure of CA2 Site Mutant of Pro-S324A
 Mono view
 Stereo pair view
|
|
A full contact list of Calcium with other atoms in the Ca binding
site number 2 of Crystal Structure of CA2 Site Mutant of Pro-S324A within 5.0Å range:
|
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Ca400
b:52.3
occ:1.00
|
OD2
|
A:ASP225
|
2.3
|
34.7
|
1.0
|
|
OD2
|
A:ASP212
|
2.3
|
48.2
|
1.0
|
|
OD1
|
A:ASP214
|
2.3
|
44.9
|
1.0
|
|
OD1
|
A:ASP222
|
2.3
|
44.8
|
1.0
|
|
O
|
A:ILE218
|
2.4
|
41.7
|
1.0
|
|
OD2
|
A:ASP216
|
3.0
|
60.8
|
1.0
|
|
OD1
|
A:ASP216
|
3.2
|
55.5
|
1.0
|
|
CG
|
A:ASP216
|
3.3
|
56.0
|
1.0
|
|
CG
|
A:ASP214
|
3.4
|
52.0
|
1.0
|
|
CG
|
A:ASP212
|
3.5
|
49.8
|
1.0
|
|
CG
|
A:ASP222
|
3.5
|
46.9
|
1.0
|
|
CG
|
A:ASP225
|
3.5
|
39.8
|
1.0
|
|
C
|
A:ILE218
|
3.6
|
42.2
|
1.0
|
|
OD2
|
A:ASP214
|
3.8
|
52.4
|
1.0
|
|
N
|
A:ILE218
|
3.9
|
45.9
|
1.0
|
|
OD1
|
A:ASP212
|
4.1
|
54.6
|
1.0
|
|
CA
|
A:ILE218
|
4.2
|
45.2
|
1.0
|
|
N
|
A:GLY215
|
4.2
|
54.1
|
1.0
|
|
N
|
A:ASP216
|
4.2
|
52.3
|
1.0
|
|
CA
|
A:CA401
|
4.3
|
60.3
|
1.0
|
|
OD1
|
A:ASP225
|
4.3
|
34.8
|
1.0
|
|
CB
|
A:ASP222
|
4.3
|
42.3
|
1.0
|
|
CB
|
A:ILE218
|
4.3
|
46.6
|
1.0
|
|
OD2
|
A:ASP222
|
4.4
|
54.4
|
1.0
|
|
N
|
A:GLY217
|
4.4
|
49.5
|
1.0
|
|
CA
|
A:ASP212
|
4.4
|
53.1
|
1.0
|
|
C
|
A:ASP212
|
4.5
|
55.4
|
1.0
|
|
CB
|
A:ASP212
|
4.5
|
53.5
|
1.0
|
|
CB
|
A:ASP225
|
4.6
|
36.5
|
1.0
|
|
CB
|
A:ASP216
|
4.6
|
52.7
|
1.0
|
|
CB
|
A:ASP214
|
4.7
|
53.9
|
1.0
|
|
N
|
A:ILE219
|
4.7
|
41.7
|
1.0
|
|
N
|
A:ASP214
|
4.7
|
57.6
|
1.0
|
|
C
|
A:ASP214
|
4.7
|
55.0
|
1.0
|
|
C
|
A:ASP216
|
4.8
|
48.7
|
1.0
|
|
CA
|
A:ASP216
|
4.8
|
50.4
|
1.0
|
|
N
|
A:LYS213
|
4.8
|
57.7
|
1.0
|
|
O
|
A:ASP212
|
4.8
|
57.2
|
1.0
|
|
CA
|
A:ILE219
|
5.0
|
40.4
|
1.0
|
|
C
|
A:GLY217
|
5.0
|
48.3
|
1.0
|
|
CA
|
A:ASP214
|
5.0
|
54.6
|
1.0
|
|
Calcium binding site 3 out
of 16 in 2zwo
Go back to
Calcium Binding Sites List in 2zwo
Calcium binding site 3 out
of 16 in the Crystal Structure of CA2 Site Mutant of Pro-S324A
 Mono view
 Stereo pair view
|
|
A full contact list of Calcium with other atoms in the Ca binding
site number 3 of Crystal Structure of CA2 Site Mutant of Pro-S324A within 5.0Å range:
|
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Ca401
b:60.3
occ:1.00
|
O
|
A:HOH607
|
2.2
|
56.9
|
1.0
|
|
OD2
|
A:ASP224
|
2.2
|
48.4
|
1.0
|
|
OD2
|
A:ASP214
|
2.7
|
52.4
|
1.0
|
|
OD2
|
A:ASP222
|
2.7
|
54.4
|
1.0
|
|
OD1
|
A:ASP216
|
2.8
|
55.5
|
1.0
|
|
OD1
|
A:ASP222
|
3.0
|
44.8
|
1.0
|
|
CG
|
A:ASP222
|
3.2
|
46.9
|
1.0
|
|
CG
|
A:ASP224
|
3.4
|
41.3
|
1.0
|
|
CG
|
A:ASP216
|
3.6
|
56.0
|
1.0
|
|
CG
|
A:ASP214
|
3.7
|
52.0
|
1.0
|
|
OD2
|
A:ASP216
|
3.8
|
60.8
|
1.0
|
|
CB
|
A:ASP224
|
3.9
|
42.2
|
1.0
|
|
OD1
|
A:ASP214
|
4.1
|
44.9
|
1.0
|
|
CA
|
A:CA400
|
4.3
|
52.3
|
1.0
|
|
OD1
|
A:ASP224
|
4.4
|
42.9
|
1.0
|
|
CB
|
A:ASP222
|
4.7
|
42.3
|
1.0
|
|
CB
|
A:ASP216
|
4.9
|
52.7
|
1.0
|
|
N
|
A:ASP224
|
4.9
|
39.8
|
1.0
|
|
Calcium binding site 4 out
of 16 in 2zwo
Go back to
Calcium Binding Sites List in 2zwo
Calcium binding site 4 out
of 16 in the Crystal Structure of CA2 Site Mutant of Pro-S324A
 Mono view
 Stereo pair view
|
|
A full contact list of Calcium with other atoms in the Ca binding
site number 4 of Crystal Structure of CA2 Site Mutant of Pro-S324A within 5.0Å range:
|
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Ca402
b:36.0
occ:1.00
|
OE1
|
A:GLU229
|
2.3
|
32.1
|
1.0
|
|
OE1
|
A:GLN110
|
2.3
|
29.2
|
1.0
|
|
O
|
A:HOH580
|
2.3
|
38.1
|
1.0
|
|
O
|
A:VAL108
|
2.4
|
34.4
|
1.0
|
|
O
|
A:ALA227
|
2.4
|
41.4
|
1.0
|
|
O
|
A:HOH447
|
2.5
|
27.8
|
1.0
|
|
OE2
|
A:GLU229
|
2.7
|
40.4
|
1.0
|
|
CD
|
A:GLU229
|
2.8
|
29.3
|
1.0
|
|
CD
|
A:GLN110
|
3.4
|
25.5
|
1.0
|
|
C
|
A:VAL108
|
3.6
|
34.7
|
1.0
|
|
C
|
A:ALA227
|
3.6
|
38.0
|
1.0
|
|
NE2
|
A:GLN110
|
3.9
|
25.5
|
1.0
|
|
O
|
A:ASP225
|
4.0
|
41.6
|
1.0
|
|
N
|
A:GLN110
|
4.2
|
28.3
|
1.0
|
|
N
|
A:ALA227
|
4.2
|
33.0
|
1.0
|
|
CG
|
A:GLU229
|
4.3
|
29.6
|
1.0
|
|
C
|
A:ALA226
|
4.3
|
33.0
|
1.0
|
|
CG2
|
A:VAL108
|
4.3
|
28.1
|
1.0
|
|
CA
|
A:ALA228
|
4.4
|
33.5
|
1.0
|
|
O
|
A:ALA226
|
4.4
|
35.3
|
1.0
|
|
N
|
A:ILE109
|
4.5
|
32.1
|
1.0
|
|
N
|
A:ALA228
|
4.5
|
34.8
|
1.0
|
|
CA
|
A:ALA227
|
4.5
|
36.1
|
1.0
|
|
CA
|
A:VAL108
|
4.5
|
33.8
|
1.0
|
|
CA
|
A:ILE109
|
4.5
|
31.8
|
1.0
|
|
C
|
A:ALA228
|
4.6
|
32.1
|
1.0
|
|
C
|
A:ILE109
|
4.7
|
31.6
|
1.0
|
|
CG
|
A:GLN110
|
4.7
|
24.1
|
1.0
|
|
N
|
A:GLU229
|
4.7
|
29.6
|
1.0
|
|
O
|
A:HOH532
|
4.7
|
40.9
|
1.0
|
|
N
|
A:GLY221
|
4.7
|
44.6
|
1.0
|
|
CB
|
A:GLN110
|
4.7
|
26.1
|
1.0
|
|
O
|
A:ASP222
|
4.9
|
40.1
|
1.0
|
|
CA
|
A:ALA226
|
4.9
|
35.0
|
1.0
|
|
O
|
A:ALA228
|
5.0
|
31.0
|
1.0
|
|
Calcium binding site 5 out
of 16 in 2zwo
Go back to
Calcium Binding Sites List in 2zwo
Calcium binding site 5 out
of 16 in the Crystal Structure of CA2 Site Mutant of Pro-S324A
 Mono view
 Stereo pair view
|
|
A full contact list of Calcium with other atoms in the Ca binding
site number 5 of Crystal Structure of CA2 Site Mutant of Pro-S324A within 5.0Å range:
|
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Ca403
b:37.2
occ:1.00
|
O
|
A:HOH604
|
2.2
|
47.6
|
1.0
|
|
OD1
|
A:ASP315
|
2.3
|
36.4
|
1.0
|
|
OD2
|
A:ASP121
|
2.3
|
37.8
|
1.0
|
|
OD2
|
A:ASP314
|
2.3
|
57.7
|
1.0
|
|
OD2
|
A:ASP119
|
2.4
|
23.7
|
1.0
|
|
CG
|
A:ASP119
|
3.1
|
25.0
|
1.0
|
|
OD1
|
A:ASP119
|
3.1
|
36.1
|
1.0
|
|
CG
|
A:ASP315
|
3.3
|
31.1
|
1.0
|
|
CG
|
A:ASP121
|
3.3
|
38.7
|
1.0
|
|
CG
|
A:ASP314
|
3.4
|
48.0
|
1.0
|
|
OD1
|
A:ASP121
|
3.7
|
40.0
|
1.0
|
|
O
|
A:HOH417
|
3.8
|
36.5
|
1.0
|
|
OD2
|
A:ASP315
|
3.8
|
31.2
|
1.0
|
|
OD1
|
A:ASP314
|
3.9
|
53.5
|
1.0
|
|
N
|
A:ASP315
|
4.1
|
28.9
|
1.0
|
|
C
|
A:ASP121
|
4.2
|
26.1
|
1.0
|
|
N
|
A:HIS122
|
4.2
|
26.2
|
1.0
|
|
O
|
A:HOH494
|
4.4
|
29.8
|
1.0
|
|
O
|
A:ASP121
|
4.4
|
26.1
|
1.0
|
|
CB
|
A:ASP314
|
4.5
|
35.2
|
1.0
|
|
CA
|
A:ASP314
|
4.5
|
30.9
|
1.0
|
|
C
|
A:ASP314
|
4.5
|
30.1
|
1.0
|
|
CA
|
A:HIS122
|
4.6
|
28.5
|
1.0
|
|
CB
|
A:ASP119
|
4.6
|
24.5
|
1.0
|
|
CB
|
A:ASP315
|
4.6
|
32.5
|
1.0
|
|
CB
|
A:ASP121
|
4.6
|
33.0
|
1.0
|
|
N
|
A:ASP121
|
4.7
|
25.2
|
1.0
|
|
CA
|
A:ASP315
|
4.8
|
30.7
|
1.0
|
|
CA
|
A:ASP121
|
4.8
|
27.0
|
1.0
|
|
C
|
A:ASP119
|
5.0
|
25.5
|
1.0
|
|
Calcium binding site 6 out
of 16 in 2zwo
Go back to
Calcium Binding Sites List in 2zwo
Calcium binding site 6 out
of 16 in the Crystal Structure of CA2 Site Mutant of Pro-S324A
 Mono view
 Stereo pair view
|
|
A full contact list of Calcium with other atoms in the Ca binding
site number 6 of Crystal Structure of CA2 Site Mutant of Pro-S324A within 5.0Å range:
|
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Ca404
b:35.6
occ:1.00
|
O
|
A:PRO375
|
2.3
|
52.2
|
1.0
|
|
O
|
A:LEU373
|
2.3
|
32.7
|
1.0
|
|
OD1
|
A:ASP372
|
2.3
|
31.8
|
1.0
|
|
O
|
A:GLY377
|
2.4
|
40.0
|
1.0
|
|
OD1
|
A:ASP379
|
2.4
|
41.6
|
1.0
|
|
O
|
A:HOH560
|
2.6
|
34.7
|
1.0
|
|
C
|
A:PRO375
|
3.4
|
48.4
|
1.0
|
|
CG
|
A:ASP372
|
3.4
|
43.1
|
1.0
|
|
C
|
A:LEU373
|
3.5
|
38.9
|
1.0
|
|
C
|
A:GLY377
|
3.6
|
43.9
|
1.0
|
|
CG
|
A:ASP379
|
3.6
|
43.9
|
1.0
|
|
N
|
A:GLY377
|
3.9
|
45.7
|
1.0
|
|
OD2
|
A:ASP372
|
4.0
|
37.2
|
1.0
|
|
C
|
A:THR376
|
4.0
|
46.9
|
1.0
|
|
O
|
A:GLY374
|
4.1
|
44.0
|
1.0
|
|
C
|
A:GLY374
|
4.1
|
46.0
|
1.0
|
|
N
|
A:LEU373
|
4.1
|
38.2
|
1.0
|
|
N
|
A:ASP379
|
4.2
|
42.6
|
1.0
|
|
CB
|
A:ASP379
|
4.2
|
40.8
|
1.0
|
|
O
|
A:GLY383
|
4.3
|
33.2
|
1.0
|
|
N
|
A:THR376
|
4.3
|
48.8
|
1.0
|
|
CA
|
A:THR376
|
4.3
|
48.0
|
1.0
|
|
C
|
A:ASP372
|
4.3
|
40.3
|
1.0
|
|
N
|
A:PRO375
|
4.3
|
47.0
|
1.0
|
|
CA
|
A:GLY377
|
4.4
|
42.5
|
1.0
|
|
O
|
A:THR376
|
4.4
|
48.5
|
1.0
|
|
CA
|
A:PRO375
|
4.4
|
48.7
|
1.0
|
|
CA
|
A:LEU373
|
4.5
|
39.3
|
1.0
|
|
N
|
A:GLY374
|
4.5
|
38.8
|
1.0
|
|
C
|
A:TRP378
|
4.5
|
42.5
|
1.0
|
|
CA
|
A:GLY374
|
4.6
|
43.2
|
1.0
|
|
CA
|
A:ASP372
|
4.6
|
40.6
|
1.0
|
|
N
|
A:TRP378
|
4.6
|
41.9
|
1.0
|
|
CB
|
A:ASP372
|
4.6
|
39.2
|
1.0
|
|
OD2
|
A:ASP379
|
4.6
|
42.2
|
1.0
|
|
CA
|
A:TRP378
|
4.8
|
40.6
|
1.0
|
|
CA
|
A:ASP379
|
4.8
|
41.3
|
1.0
|
|
O
|
A:ASP372
|
4.9
|
41.5
|
1.0
|
|
CB
|
A:TYR382
|
4.9
|
34.1
|
1.0
|
|
O
|
A:HOH664
|
4.9
|
41.8
|
1.0
|
|
CB
|
A:PRO375
|
4.9
|
48.0
|
1.0
|
|
O
|
A:TYR384
|
4.9
|
39.9
|
1.0
|
|
Calcium binding site 7 out
of 16 in 2zwo
Go back to
Calcium Binding Sites List in 2zwo
Calcium binding site 7 out
of 16 in the Crystal Structure of CA2 Site Mutant of Pro-S324A
 Mono view
 Stereo pair view
|
|
A full contact list of Calcium with other atoms in the Ca binding
site number 7 of Crystal Structure of CA2 Site Mutant of Pro-S324A within 5.0Å range:
|
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
B:Ca399
b:44.6
occ:1.00
|
O
|
B:VAL170
|
2.3
|
37.2
|
1.0
|
|
O
|
B:LEU164
|
2.4
|
40.3
|
1.0
|
|
OD1
|
B:ASN166
|
2.4
|
47.8
|
1.0
|
|
OD1
|
B:ASP124
|
2.5
|
33.8
|
1.0
|
|
OE1
|
B:GLN84
|
2.5
|
40.4
|
1.0
|
|
O
|
B:ILE168
|
2.6
|
44.8
|
1.0
|
|
OD2
|
B:ASP124
|
2.6
|
29.0
|
1.0
|
|
CG
|
B:ASP124
|
2.9
|
35.7
|
1.0
|
|
CD
|
B:GLN84
|
3.4
|
48.3
|
1.0
|
|
CG
|
B:ASN166
|
3.4
|
49.8
|
1.0
|
|
C
|
B:VAL170
|
3.5
|
37.5
|
1.0
|
|
C
|
B:LEU164
|
3.5
|
41.1
|
1.0
|
|
CG
|
B:GLN84
|
3.7
|
43.0
|
1.0
|
|
C
|
B:ILE168
|
3.7
|
44.2
|
1.0
|
|
N
|
B:VAL170
|
3.8
|
40.9
|
1.0
|
|
ND2
|
B:ASN166
|
4.0
|
32.7
|
1.0
|
|
N
|
B:ASN166
|
4.0
|
50.1
|
1.0
|
|
C
|
B:GLY169
|
4.2
|
40.8
|
1.0
|
|
N
|
B:LEU164
|
4.2
|
41.3
|
1.0
|
|
CA
|
B:VAL170
|
4.3
|
37.1
|
1.0
|
|
CA
|
B:LEU164
|
4.3
|
39.9
|
1.0
|
|
CB
|
B:ASP124
|
4.4
|
40.2
|
1.0
|
|
N
|
B:VAL171
|
4.4
|
37.9
|
1.0
|
|
NE2
|
B:GLN84
|
4.5
|
47.7
|
1.0
|
|
CA
|
B:GLY169
|
4.5
|
41.0
|
1.0
|
|
N
|
B:ASN165
|
4.5
|
44.6
|
1.0
|
|
CA
|
B:VAL171
|
4.5
|
34.9
|
1.0
|
|
CB
|
B:GLN84
|
4.6
|
47.9
|
1.0
|
|
N
|
B:GLY169
|
4.6
|
43.8
|
1.0
|
|
CB
|
B:ASN166
|
4.6
|
48.9
|
1.0
|
|
CA
|
B:ASN165
|
4.6
|
47.1
|
1.0
|
|
N
|
B:ILE168
|
4.6
|
49.0
|
1.0
|
|
CG1
|
B:ILE168
|
4.6
|
47.7
|
1.0
|
|
CA
|
B:ASN166
|
4.7
|
49.7
|
1.0
|
|
CB
|
B:VAL171
|
4.7
|
39.5
|
1.0
|
|
CB
|
B:LEU164
|
4.7
|
40.8
|
1.0
|
|
CA
|
B:ILE168
|
4.7
|
45.1
|
1.0
|
|
C
|
B:ASN165
|
4.8
|
48.8
|
1.0
|
|
C
|
B:ALA163
|
4.8
|
37.5
|
1.0
|
|
O
|
B:GLY169
|
4.8
|
42.1
|
1.0
|
|
C
|
B:ASN166
|
4.8
|
52.6
|
1.0
|
|
O
|
B:ASN166
|
4.9
|
53.5
|
1.0
|
|
O
|
B:ALA163
|
4.9
|
42.4
|
1.0
|
|
Calcium binding site 8 out
of 16 in 2zwo
Go back to
Calcium Binding Sites List in 2zwo
Calcium binding site 8 out
of 16 in the Crystal Structure of CA2 Site Mutant of Pro-S324A
 Mono view
 Stereo pair view
|
|
A full contact list of Calcium with other atoms in the Ca binding
site number 8 of Crystal Structure of CA2 Site Mutant of Pro-S324A within 5.0Å range:
|
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
B:Ca400
b:34.8
occ:1.00
|
OD1
|
B:ASP222
|
2.2
|
37.2
|
1.0
|
|
OD2
|
B:ASP225
|
2.3
|
28.9
|
1.0
|
|
OD1
|
B:ASP212
|
2.3
|
29.1
|
1.0
|
|
O
|
B:ILE218
|
2.3
|
34.4
|
1.0
|
|
OD1
|
B:ASP216
|
2.4
|
32.7
|
1.0
|
|
OD2
|
B:ASP214
|
2.4
|
37.4
|
1.0
|
|
CG
|
B:ASP216
|
3.3
|
32.8
|
1.0
|
|
CG
|
B:ASP222
|
3.4
|
27.9
|
1.0
|
|
C
|
B:ILE218
|
3.4
|
29.1
|
1.0
|
|
CG
|
B:ASP214
|
3.5
|
30.1
|
1.0
|
|
CG
|
B:ASP225
|
3.5
|
28.7
|
1.0
|
|
CG
|
B:ASP212
|
3.5
|
33.6
|
1.0
|
|
OD2
|
B:ASP216
|
3.7
|
16.2
|
1.0
|
|
OD1
|
B:ASP214
|
3.8
|
32.4
|
1.0
|
|
N
|
B:ILE218
|
3.9
|
31.5
|
1.0
|
|
CB
|
B:ASP222
|
4.1
|
28.7
|
1.0
|
|
CA
|
B:ILE218
|
4.1
|
30.4
|
1.0
|
|
N
|
B:ASP216
|
4.3
|
29.8
|
1.0
|
|
CA
|
B:CA401
|
4.3
|
28.9
|
1.0
|
|
OD2
|
B:ASP212
|
4.3
|
25.4
|
1.0
|
|
CA
|
B:ASP212
|
4.3
|
32.2
|
1.0
|
|
OD1
|
B:ASP225
|
4.4
|
32.2
|
1.0
|
|
OD2
|
B:ASP222
|
4.4
|
32.2
|
1.0
|
|
CB
|
B:ASP225
|
4.4
|
26.1
|
1.0
|
|
CB
|
B:ILE218
|
4.4
|
31.7
|
1.0
|
|
CB
|
B:ASP216
|
4.5
|
25.7
|
1.0
|
|
N
|
B:ASP214
|
4.5
|
31.6
|
1.0
|
|
CB
|
B:ASP212
|
4.5
|
28.7
|
1.0
|
|
N
|
B:ILE219
|
4.5
|
32.4
|
1.0
|
|
C
|
B:ASP212
|
4.6
|
27.9
|
1.0
|
|
CA
|
B:ASP216
|
4.7
|
29.7
|
1.0
|
|
N
|
B:GLY217
|
4.7
|
32.2
|
1.0
|
|
CB
|
B:ASP214
|
4.8
|
31.5
|
1.0
|
|
N
|
B:LYS213
|
4.8
|
31.7
|
1.0
|
|
CA
|
B:ILE219
|
4.8
|
29.7
|
1.0
|
|
N
|
B:GLY215
|
4.8
|
29.1
|
1.0
|
|
C
|
B:ASP216
|
4.9
|
32.1
|
1.0
|
|
CA
|
B:ASP214
|
5.0
|
32.0
|
1.0
|
|
C
|
B:GLY217
|
5.0
|
35.3
|
1.0
|
|
Calcium binding site 9 out
of 16 in 2zwo
Go back to
Calcium Binding Sites List in 2zwo
Calcium binding site 9 out
of 16 in the Crystal Structure of CA2 Site Mutant of Pro-S324A
 Mono view
 Stereo pair view
|
|
A full contact list of Calcium with other atoms in the Ca binding
site number 9 of Crystal Structure of CA2 Site Mutant of Pro-S324A within 5.0Å range:
|
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
B:Ca401
b:28.9
occ:1.00
|
OD2
|
B:ASP216
|
2.2
|
16.2
|
1.0
|
|
OD2
|
B:ASP224
|
2.3
|
25.5
|
1.0
|
|
OD1
|
B:ASP214
|
2.4
|
32.4
|
1.0
|
|
OD2
|
B:ASP222
|
2.5
|
32.2
|
1.0
|
|
OD1
|
B:ASP222
|
2.8
|
37.2
|
1.0
|
|
CG
|
B:ASP222
|
3.0
|
27.9
|
1.0
|
|
CG
|
B:ASP224
|
3.3
|
32.9
|
1.0
|
|
CG
|
B:ASP216
|
3.5
|
32.8
|
1.0
|
|
CB
|
B:ASP224
|
3.5
|
22.8
|
1.0
|
|
CG
|
B:ASP214
|
3.5
|
30.1
|
1.0
|
|
OD2
|
B:ASP214
|
4.0
|
37.4
|
1.0
|
|
OD1
|
B:ASP216
|
4.1
|
32.7
|
1.0
|
|
CA
|
B:CA400
|
4.3
|
34.8
|
1.0
|
|
OD1
|
B:ASP224
|
4.4
|
22.1
|
1.0
|
|
CB
|
B:ASP222
|
4.5
|
28.7
|
1.0
|
|
CB
|
B:ASP216
|
4.5
|
25.7
|
1.0
|
|
N
|
B:ASP224
|
4.6
|
29.3
|
1.0
|
|
CA
|
B:ASP224
|
4.7
|
29.1
|
1.0
|
|
CB
|
B:ASP214
|
4.8
|
31.5
|
1.0
|
|
Calcium binding site 10 out
of 16 in 2zwo
Go back to
Calcium Binding Sites List in 2zwo
Calcium binding site 10 out
of 16 in the Crystal Structure of CA2 Site Mutant of Pro-S324A
 Mono view
 Stereo pair view
|
|
A full contact list of Calcium with other atoms in the Ca binding
site number 10 of Crystal Structure of CA2 Site Mutant of Pro-S324A within 5.0Å range:
|
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
B:Ca402
b:47.6
occ:1.00
|
OD1
|
B:ASP372
|
2.1
|
46.4
|
1.0
|
|
OD1
|
B:ASP379
|
2.2
|
37.8
|
1.0
|
|
O
|
B:LEU373
|
2.2
|
38.7
|
1.0
|
|
O
|
B:GLY377
|
2.6
|
45.5
|
1.0
|
|
O
|
B:PRO375
|
2.8
|
51.5
|
1.0
|
|
CG
|
B:ASP372
|
3.3
|
42.9
|
1.0
|
|
C
|
B:LEU373
|
3.4
|
43.8
|
1.0
|
|
CG
|
B:ASP379
|
3.5
|
41.2
|
1.0
|
|
N
|
B:LEU373
|
3.5
|
44.4
|
1.0
|
|
C
|
B:GLY377
|
3.6
|
48.2
|
1.0
|
|
C
|
B:PRO375
|
3.9
|
49.5
|
1.0
|
|
N
|
B:GLY377
|
3.9
|
50.9
|
1.0
|
|
OD2
|
B:ASP372
|
4.0
|
47.0
|
1.0
|
|
C
|
B:ASP372
|
4.0
|
47.1
|
1.0
|
|
CA
|
B:LEU373
|
4.2
|
42.7
|
1.0
|
|
C
|
B:GLY374
|
4.2
|
42.0
|
1.0
|
|
CA
|
B:GLY377
|
4.2
|
50.0
|
1.0
|
|
O
|
B:GLY374
|
4.2
|
40.3
|
1.0
|
|
OD2
|
B:ASP379
|
4.2
|
34.9
|
1.0
|
|
C
|
B:THR376
|
4.3
|
53.9
|
1.0
|
|
CA
|
B:ASP372
|
4.3
|
44.8
|
1.0
|
|
N
|
B:GLY374
|
4.4
|
44.0
|
1.0
|
|
CB
|
B:ASP372
|
4.4
|
45.0
|
1.0
|
|
CA
|
B:GLY374
|
4.4
|
42.5
|
1.0
|
|
CB
|
B:ASP379
|
4.5
|
43.5
|
1.0
|
|
N
|
B:ASP379
|
4.5
|
47.4
|
1.0
|
|
N
|
B:PRO375
|
4.5
|
41.6
|
1.0
|
|
CA
|
B:THR376
|
4.5
|
55.4
|
1.0
|
|
O
|
B:GLY383
|
4.5
|
42.3
|
1.0
|
|
N
|
B:TRP378
|
4.6
|
46.5
|
1.0
|
|
O
|
B:ASP372
|
4.6
|
47.4
|
1.0
|
|
N
|
B:THR376
|
4.7
|
52.8
|
1.0
|
|
O
|
B:TYR384
|
4.7
|
34.3
|
1.0
|
|
C
|
B:TRP378
|
4.7
|
45.0
|
1.0
|
|
O
|
B:THR376
|
4.7
|
52.2
|
1.0
|
|
CA
|
B:PRO375
|
4.8
|
45.6
|
1.0
|
|
CA
|
B:TRP378
|
4.8
|
44.1
|
1.0
|
|
CB
|
B:TYR382
|
5.0
|
39.8
|
1.0
|
|
Reference:
Y.Takeuchi,
S.Tanaka,
H.Matsumura,
Y.Koga,
K.Takano,
S.Kanaya.
Requirement of A Unique Ca(2+)-Binding Loop For Folding of Tk-Subtilisin From A Hyperthermophilic Archaeon. Biochemistry V. 48 10637 2009.
ISSN: ISSN 0006-2960
PubMed: 19813760
DOI: 10.1021/BI901334B
Page generated: Sat Dec 12 04:02:12 2020
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