Calcium in PDB 3axi: Crystal Structure of Isomaltase in Complex with Maltose

All present enzymatic activity of Crystal Structure of Isomaltase in Complex with Maltose:
3.2.1.10;

The structure of Crystal Structure of Isomaltase in Complex with Maltose, PDB code: 3axi was solved by K.Yamamoto, H.Miyake, M.Kusunoki, S.Osaki, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	18.83 / 1.40
Space group	C 1 2 1
Cell size a, b, c (Å), α, β, γ (°)	95.474, 115.373, 61.594, 90.00, 91.07, 90.00
R / Rfree (%)	17.9 / 19.4

The binding sites of Calcium atom in the Crystal Structure of Isomaltase in Complex with Maltose (pdb code 3axi). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total only one binding site of Calcium was determined in the Crystal Structure of Isomaltase in Complex with Maltose, PDB code: 3axi:

Calcium binding site 1 out of 1 in the Crystal Structure of Isomaltase in Complex with Maltose


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Crystal Structure of Isomaltase in Complex with Maltose within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca602 b:8.8 occ:1.00 O A:TRP36 2.3 8.7 1.0 OD1 A:ASN32 2.3 11.8 1.0 OD1 A:ASP30 2.3 9.4 1.0 O A:HOH1594 2.3 12.7 1.0 OD1 A:ASP34 2.3 9.6 1.0 OD2 A:ASP38 2.4 8.3 1.0 CG A:ASP34 3.2 10.7 1.0 CG A:ASP30 3.3 9.0 1.0 CG A:ASN32 3.3 11.2 1.0 C A:TRP36 3.4 8.3 1.0 CG A:ASP38 3.5 8.8 1.0 OD2 A:ASP34 3.6 12.1 1.0 ND2 A:ASN32 3.8 14.0 1.0 CB A:ASP38 3.9 8.2 1.0 OD2 A:ASP30 4.1 9.7 1.0 N A:TRP36 4.1 8.6 1.0 CA A:ASP30 4.1 8.9 1.0 CB A:ASP30 4.2 8.7 1.0 N A:ASN32 4.2 10.0 1.0 CA A:TRP36 4.2 8.6 1.0 N A:SER31 4.2 10.2 1.0 C A:GLY37 4.3 7.4 1.0 O A:GLY37 4.3 7.8 1.0 CB A:TRP36 4.3 9.4 1.0 N A:ASP34 4.4 10.7 1.0 CB A:ASP34 4.5 10.7 1.0 N A:GLY37 4.5 7.4 1.0 OD1 A:ASP38 4.6 8.9 1.0 CB A:ASN32 4.6 10.7 1.0 N A:ASP38 4.6 7.4 1.0 C A:ASP30 4.6 9.8 1.0 CA A:GLY37 4.6 7.5 1.0 CA A:ASN32 4.7 10.5 1.0 O A:THR83 4.7 11.0 1.0 N A:ASP33 4.7 10.7 1.0 CA A:ASP34 4.8 10.7 1.0 C A:ASN32 4.8 10.6 1.0 N A:GLY35 4.8 10.2 1.0 CA A:ASP38 4.9 8.1 1.0 C A:ASP34 5.0 10.2 1.0

K.Yamamoto, H.Miyake, M.Kusunoki, S.Osaki. Steric Hindrance By 2 Amino Acid Residues Determines the Substrate Specificity of Isomaltase From Saccharomyces Cerevisiae J.Biosci.Bioeng. V. 112 545 2011.
ISSN: ISSN 1389-1723
PubMed: 21925939
DOI: 10.1016/J.JBIOSC.2011.08.016