Calcium in PDB 3brx: Crystal Structure of Calcium-Bound Cotton Annexin GH1

The structure of Crystal Structure of Calcium-Bound Cotton Annexin GH1, PDB code: 3brx was solved by N.-J.Hu, A.Hofmann, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	35.44 / 2.50
Space group	P 3 2 1
Cell size a, b, c (Å), α, β, γ (°)	132.663, 132.663, 61.338, 90.00, 90.00, 120.00
R / Rfree (%)	19.3 / 25.2

The binding sites of Calcium atom in the Crystal Structure of Calcium-Bound Cotton Annexin GH1 (pdb code 3brx). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 3 binding sites of Calcium where determined in the Crystal Structure of Calcium-Bound Cotton Annexin GH1, PDB code: 3brx:
Jump to Calcium binding site number: 1; 2; 3;

Calcium binding site 1 out of 3 in the Crystal Structure of Calcium-Bound Cotton Annexin GH1


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Crystal Structure of Calcium-Bound Cotton Annexin GH1 within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca327 b:44.9 occ:1.00 O A:PHE29 2.4 31.5 1.0 O A:GLY33 2.5 28.9 1.0 O A:GLY31 2.6 30.1 1.0 OE2 A:GLU73 2.7 43.5 1.0 O A:HOH332 2.9 27.2 1.0 OE1 A:GLU73 3.0 44.1 1.0 CD A:GLU73 3.2 42.7 1.0 C A:PHE29 3.5 31.8 1.0 C A:GLY33 3.6 29.3 1.0 C A:GLY31 3.7 30.4 1.0 N A:GLY33 3.8 30.2 1.0 N A:GLY31 4.0 30.0 1.0 C A:SER30 4.1 30.6 1.0 O A:HOH565 4.2 35.8 1.0 O A:SER30 4.2 30.5 1.0 CA A:PHE29 4.2 32.2 1.0 CA A:GLY33 4.3 29.6 1.0 N A:SER30 4.4 31.5 1.0 CA A:GLY31 4.4 30.2 1.0 C A:TRP32 4.5 31.0 1.0 OG1 A:THR34 4.5 30.8 1.0 CA A:SER30 4.6 31.0 1.0 N A:TRP32 4.6 30.6 1.0 CA A:TRP32 4.7 31.0 1.0 N A:THR34 4.7 28.8 1.0 CG A:GLU73 4.7 40.1 1.0 CB A:PHE29 4.8 32.1 1.0 CA A:THR34 5.0 28.7 1.0

Calcium binding site 2 out of 3 in the Crystal Structure of Calcium-Bound Cotton Annexin GH1


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of Crystal Structure of Calcium-Bound Cotton Annexin GH1 within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca328 b:58.9 occ:1.00 O A:ARG261 2.1 42.6 1.0 O A:GLY263 2.1 39.1 1.0 OD1 A:ASP303 2.4 38.8 1.0 O A:ILE259 2.6 32.3 1.0 OD2 A:ASP303 2.8 37.8 1.0 CG A:ASP303 2.9 36.5 1.0 C A:ARG261 3.2 42.5 1.0 C A:GLY263 3.3 38.9 1.0 C A:ILE259 3.7 32.0 1.0 N A:ARG261 3.7 39.6 1.0 N A:GLY263 3.8 42.5 1.0 C A:ARG262 4.0 43.7 1.0 CA A:ARG261 4.1 41.4 1.0 CA A:GLY263 4.1 40.6 1.0 N A:ARG262 4.2 43.6 1.0 N A:THR264 4.3 36.5 1.0 CA A:ARG262 4.3 44.6 1.0 CG A:ARG261 4.3 41.3 1.0 CB A:ASP303 4.4 33.6 1.0 OG1 A:THR264 4.5 36.1 1.0 O A:ARG262 4.5 44.3 1.0 CA A:ILE259 4.5 29.6 1.0 N A:ASN260 4.7 34.6 1.0 CB A:ARG261 4.8 41.4 1.0 C A:ASN260 4.8 37.9 1.0 CA A:THR264 4.8 34.3 1.0 O A:HOH571 4.8 38.2 1.0 CA A:ASN260 4.8 36.7 1.0

Calcium binding site 3 out of 3 in the Crystal Structure of Calcium-Bound Cotton Annexin GH1


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 3 of Crystal Structure of Calcium-Bound Cotton Annexin GH1 within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca329 b:47.7 occ:1.00 O A:VAL301 2.2 33.7 1.0 O A:HOH459 2.4 28.8 1.0 O2 A:PO4324 2.5 76.1 1.0 O A:THR304 2.7 30.8 1.0 OE1 A:GLU309 2.9 29.9 1.0 O A:HOH421 3.0 38.6 1.0 OE2 A:GLU309 3.0 31.0 1.0 CD A:GLU309 3.3 26.4 1.0 C A:VAL301 3.3 32.5 1.0 C A:THR304 3.9 30.9 1.0 P A:PO4324 3.9 76.9 1.0 N A:LYS302 4.0 32.9 1.0 CD2 A:HIS305 4.1 36.3 1.0 O3 A:PO4324 4.3 77.0 1.0 CA A:VAL301 4.3 31.4 1.0 CG2 A:VAL301 4.5 31.1 1.0 CA A:HIS305 4.5 30.7 1.0 N A:HIS305 4.6 30.7 1.0 O A:LYS302 4.6 33.4 1.0 O1 A:PO4324 4.7 76.8 1.0 CG A:GLU309 4.7 24.9 1.0 CG A:HIS305 4.7 34.1 1.0 N A:THR304 4.7 31.0 1.0 NE2 A:HIS305 4.8 37.0 1.0 CA A:LYS302 4.8 33.6 1.0 CB A:VAL301 4.8 31.1 1.0 C A:LYS302 4.8 33.3 1.0 O4 A:PO4324 4.9 77.5 1.0 CA A:THR304 4.9 30.5 1.0

N.-J.Hu, A.M.Yusof, A.Winter, A.Osman, A.K.Reeve, A.Hofmann. The Crystal Structure of Calcium-Bound Annexin GH1 From Gossypium Hirsutum and Its Implications For Membrane Binding Mechanisms of Plant Annexins. J.Biol.Chem. V. 283 18314 2008.
ISSN: ISSN 0021-9258
PubMed: 18441010
DOI: 10.1074/JBC.M801051200