Calcium in PDB 3byn: Crystal Structure of B. Subtilis Levansucrase Mutant E342A Bound to Raffinose

All present enzymatic activity of Crystal Structure of B. Subtilis Levansucrase Mutant E342A Bound to Raffinose:
2.4.1.10;

The structure of Crystal Structure of B. Subtilis Levansucrase Mutant E342A Bound to Raffinose, PDB code: 3byn was solved by K.Futterer, G.Meng, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	30.00 / 2.10
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	51.211, 66.700, 124.042, 90.00, 90.00, 90.00
R / Rfree (%)	17.9 / 22.4

The binding sites of Calcium atom in the Crystal Structure of B. Subtilis Levansucrase Mutant E342A Bound to Raffinose (pdb code 3byn). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total only one binding site of Calcium was determined in the Crystal Structure of B. Subtilis Levansucrase Mutant E342A Bound to Raffinose, PDB code: 3byn:

Calcium binding site 1 out of 1 in the Crystal Structure of B. Subtilis Levansucrase Mutant E342A Bound to Raffinose


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Crystal Structure of B. Subtilis Levansucrase Mutant E342A Bound to Raffinose within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca475 b:13.2 occ:1.00 O A:LEU308 2.3 13.1 1.0 OE1 A:GLN272 2.3 13.8 1.0 OD1 A:ASP241 2.4 14.7 1.0 OD1 A:ASN310 2.4 14.9 1.0 O A:HOH632 2.4 6.7 1.0 OD1 A:ASP339 2.6 14.1 1.0 OD2 A:ASP339 2.7 13.6 1.0 CG A:ASP339 3.0 14.7 1.0 CG A:ASP241 3.4 15.1 1.0 CD A:GLN272 3.5 14.8 1.0 CG A:ASN310 3.5 13.4 1.0 C A:LEU308 3.5 12.9 1.0 OD2 A:ASP241 3.8 14.1 1.0 ND2 A:ASN264 4.0 14.6 1.0 N A:ASN310 4.1 13.5 1.0 NE2 A:GLN272 4.2 15.0 1.0 O A:ASN242 4.2 14.9 1.0 ND2 A:ASN310 4.2 12.3 1.0 CA A:LEU308 4.3 12.7 1.0 CB A:GLN272 4.3 14.5 1.0 N A:ASN242 4.4 15.1 1.0 CB A:ASP339 4.5 14.9 1.0 CG A:GLN272 4.5 14.4 1.0 N A:ALA309 4.5 12.9 1.0 NE2 A:GLN226 4.5 12.9 1.0 CB A:ASN310 4.6 13.6 1.0 CB A:LEU308 4.6 12.7 1.0 CG A:ASN264 4.6 15.0 1.0 CA A:ALA309 4.7 13.0 1.0 OE1 A:GLN226 4.7 14.3 1.0 C A:ALA309 4.7 13.3 1.0 CB A:ASP241 4.8 15.2 1.0 CA A:ASN310 4.8 13.6 1.0 CB A:ASN264 4.8 14.4 1.0 C A:ASN242 4.8 15.1 1.0 CD A:GLN226 4.9 14.0 1.0 O A:HOH509 5.0 8.9 1.0 CA A:ASP241 5.0 15.3 1.0

G.Meng, K.Futterer. Donor Substrate Recognition in the Raffinose-Bound E342A Mutant of Fructosyltransferase Bacillus Subtilis Levansucrase To Be Published.