Calcium in PDB 3ci0: The Crystal Structure of the Gspk-Gspi-Gspj Complex From Enterotoxigenic Escherichia Coli Type 2 Secretion System
Protein crystallography data
The structure of The Crystal Structure of the Gspk-Gspi-Gspj Complex From Enterotoxigenic Escherichia Coli Type 2 Secretion System, PDB code: 3ci0
was solved by
K.V.Korotkov,
W.G.J.Hol,
with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:
Resolution Low / High (Å)
|
35.96 /
2.20
|
Space group
|
P 41 21 2
|
Cell size a, b, c (Å), α, β, γ (°)
|
96.118,
96.118,
108.436,
90.00,
90.00,
90.00
|
R / Rfree (%)
|
19.2 /
25.9
|
Other elements in 3ci0:
The structure of The Crystal Structure of the Gspk-Gspi-Gspj Complex From Enterotoxigenic Escherichia Coli Type 2 Secretion System also contains other interesting chemical elements:
Calcium Binding Sites:
The binding sites of Calcium atom in the The Crystal Structure of the Gspk-Gspi-Gspj Complex From Enterotoxigenic Escherichia Coli Type 2 Secretion System
(pdb code 3ci0). This binding sites where shown within
5.0 Angstroms radius around Calcium atom.
In total 3 binding sites of Calcium where determined in the
The Crystal Structure of the Gspk-Gspi-Gspj Complex From Enterotoxigenic Escherichia Coli Type 2 Secretion System, PDB code: 3ci0:
Jump to Calcium binding site number:
1;
2;
3;
Calcium binding site 1 out
of 3 in 3ci0
Go back to
Calcium Binding Sites List in 3ci0
Calcium binding site 1 out
of 3 in the The Crystal Structure of the Gspk-Gspi-Gspj Complex From Enterotoxigenic Escherichia Coli Type 2 Secretion System
Mono view
Stereo pair view
|
A full contact list of Calcium with other atoms in the Ca binding
site number 1 of The Crystal Structure of the Gspk-Gspi-Gspj Complex From Enterotoxigenic Escherichia Coli Type 2 Secretion System within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
K:Ca401
b:73.1
occ:1.00
|
OD1
|
K:ASP144
|
2.3
|
76.0
|
1.0
|
OE1
|
K:GLU139
|
2.3
|
73.8
|
1.0
|
OD2
|
K:ASP142
|
2.3
|
73.9
|
1.0
|
O
|
K:SER146
|
2.4
|
75.9
|
1.0
|
OE1
|
K:GLN148
|
2.4
|
74.4
|
1.0
|
OE2
|
K:GLU154
|
2.5
|
73.7
|
1.0
|
OD1
|
K:ASP142
|
2.5
|
72.4
|
1.0
|
CG
|
K:ASP142
|
2.7
|
74.6
|
1.0
|
CG
|
K:ASP144
|
3.0
|
78.0
|
1.0
|
OD2
|
K:ASP144
|
3.1
|
80.0
|
1.0
|
CD
|
K:GLN148
|
3.4
|
76.7
|
1.0
|
CD
|
K:GLU139
|
3.5
|
74.0
|
1.0
|
C
|
K:SER146
|
3.6
|
76.8
|
1.0
|
NE2
|
K:GLN148
|
3.7
|
75.8
|
1.0
|
CD
|
K:GLU154
|
3.7
|
76.2
|
1.0
|
CA
|
K:CA402
|
3.8
|
71.1
|
1.0
|
O
|
K:HOH435
|
4.1
|
57.5
|
1.0
|
O
|
K:ASP144
|
4.1
|
75.5
|
1.0
|
CA
|
K:GLU139
|
4.2
|
74.5
|
1.0
|
OE2
|
K:GLU139
|
4.2
|
75.1
|
1.0
|
CB
|
K:ASP142
|
4.3
|
75.4
|
1.0
|
CG
|
K:GLU154
|
4.4
|
78.0
|
1.0
|
N
|
K:SER146
|
4.4
|
77.1
|
1.0
|
CB
|
K:GLU139
|
4.4
|
74.4
|
1.0
|
N
|
K:VAL147
|
4.5
|
76.9
|
1.0
|
CB
|
K:ASP144
|
4.5
|
77.2
|
1.0
|
O
|
K:GLU139
|
4.5
|
74.0
|
1.0
|
C
|
K:ASP144
|
4.5
|
76.9
|
1.0
|
CG
|
K:GLU139
|
4.5
|
74.8
|
1.0
|
CA
|
K:VAL147
|
4.5
|
77.1
|
1.0
|
CA
|
K:SER146
|
4.6
|
77.5
|
1.0
|
OE1
|
K:GLU154
|
4.6
|
72.7
|
1.0
|
N
|
K:GLN148
|
4.7
|
77.7
|
1.0
|
CG
|
K:GLN148
|
4.8
|
77.0
|
1.0
|
C
|
K:VAL147
|
4.9
|
77.4
|
1.0
|
C
|
K:GLU139
|
4.9
|
74.5
|
1.0
|
CA
|
K:ASP144
|
4.9
|
77.0
|
1.0
|
|
Calcium binding site 2 out
of 3 in 3ci0
Go back to
Calcium Binding Sites List in 3ci0
Calcium binding site 2 out
of 3 in the The Crystal Structure of the Gspk-Gspi-Gspj Complex From Enterotoxigenic Escherichia Coli Type 2 Secretion System
Mono view
Stereo pair view
|
A full contact list of Calcium with other atoms in the Ca binding
site number 2 of The Crystal Structure of the Gspk-Gspi-Gspj Complex From Enterotoxigenic Escherichia Coli Type 2 Secretion System within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
I:Ca115
b:69.8
occ:1.00
|
O
|
I:HOH137
|
2.3
|
69.2
|
1.0
|
O
|
I:ASP68
|
2.4
|
66.8
|
1.0
|
O
|
J:HOH240
|
2.7
|
56.0
|
1.0
|
O
|
J:HOH211
|
2.8
|
63.6
|
1.0
|
C
|
I:ASP68
|
3.6
|
67.0
|
1.0
|
OE2
|
J:GLU181
|
3.8
|
69.2
|
1.0
|
CB
|
I:ASP68
|
4.2
|
67.8
|
1.0
|
CA
|
I:ASP68
|
4.3
|
67.3
|
1.0
|
OE1
|
J:GLU183
|
4.3
|
72.4
|
1.0
|
N
|
I:ASP68
|
4.4
|
66.3
|
1.0
|
N
|
I:MSE69
|
4.5
|
67.6
|
1.0
|
CD
|
J:GLU181
|
4.6
|
63.6
|
1.0
|
CA
|
I:MSE69
|
4.6
|
68.7
|
1.0
|
CG
|
J:GLU181
|
4.7
|
62.9
|
1.0
|
CB
|
J:GLU183
|
4.8
|
61.0
|
1.0
|
CE
|
I:MSE69
|
4.8
|
48.5
|
1.0
|
O
|
I:HOH116
|
4.8
|
44.0
|
1.0
|
O
|
J:HOH241
|
4.9
|
72.7
|
1.0
|
O
|
J:GLU181
|
5.0
|
55.9
|
1.0
|
|
Calcium binding site 3 out
of 3 in 3ci0
Go back to
Calcium Binding Sites List in 3ci0
Calcium binding site 3 out
of 3 in the The Crystal Structure of the Gspk-Gspi-Gspj Complex From Enterotoxigenic Escherichia Coli Type 2 Secretion System
Mono view
Stereo pair view
|
A full contact list of Calcium with other atoms in the Ca binding
site number 3 of The Crystal Structure of the Gspk-Gspi-Gspj Complex From Enterotoxigenic Escherichia Coli Type 2 Secretion System within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
K:Ca402
b:71.1
occ:1.00
|
O
|
K:GLU139
|
2.3
|
74.0
|
1.0
|
OD1
|
K:ASP142
|
2.3
|
72.4
|
1.0
|
O
|
K:ASP144
|
2.3
|
75.5
|
1.0
|
OD1
|
K:ASN169
|
2.4
|
73.1
|
1.0
|
OE1
|
K:GLU154
|
2.4
|
72.7
|
1.0
|
O
|
K:ASP142
|
2.4
|
74.1
|
1.0
|
OE2
|
K:GLU154
|
2.4
|
73.7
|
1.0
|
CD
|
K:GLU154
|
2.7
|
76.2
|
1.0
|
CG
|
K:ASN169
|
3.3
|
73.7
|
1.0
|
C
|
K:ASP142
|
3.4
|
75.6
|
1.0
|
C
|
K:GLU139
|
3.5
|
74.5
|
1.0
|
CG
|
K:ASP142
|
3.5
|
74.6
|
1.0
|
C
|
K:ASP144
|
3.6
|
76.9
|
1.0
|
CA
|
K:CA401
|
3.8
|
73.1
|
1.0
|
OD1
|
K:ASP144
|
4.0
|
76.0
|
1.0
|
ND2
|
K:ASN169
|
4.1
|
73.2
|
1.0
|
CA
|
K:ASP142
|
4.1
|
75.1
|
1.0
|
CA
|
K:GLU139
|
4.2
|
74.5
|
1.0
|
N
|
K:ASP144
|
4.2
|
76.8
|
1.0
|
CB
|
K:ASN169
|
4.2
|
73.1
|
1.0
|
N
|
K:ASP142
|
4.2
|
75.3
|
1.0
|
CG
|
K:GLU154
|
4.3
|
78.0
|
1.0
|
O
|
K:HOH415
|
4.3
|
58.9
|
1.0
|
CA
|
K:ASN169
|
4.4
|
72.8
|
1.0
|
C
|
K:GLU143
|
4.4
|
77.1
|
1.0
|
CB
|
K:ASP142
|
4.4
|
75.4
|
1.0
|
N
|
K:GLU143
|
4.4
|
76.1
|
1.0
|
OD2
|
K:ASP142
|
4.4
|
73.9
|
1.0
|
N
|
K:ARG145
|
4.5
|
77.4
|
1.0
|
CA
|
K:ASP144
|
4.5
|
77.0
|
1.0
|
CA
|
K:ARG145
|
4.5
|
77.9
|
1.0
|
N
|
K:PHE140
|
4.5
|
74.9
|
1.0
|
C
|
K:PHE140
|
4.6
|
75.5
|
1.0
|
O
|
K:PHE140
|
4.6
|
75.9
|
1.0
|
CG
|
K:ASP144
|
4.6
|
78.0
|
1.0
|
CA
|
K:GLU143
|
4.8
|
77.6
|
1.0
|
CA
|
K:PHE140
|
4.8
|
75.4
|
1.0
|
CB
|
K:GLU139
|
4.8
|
74.4
|
1.0
|
O
|
K:GLU143
|
4.8
|
77.3
|
1.0
|
OE1
|
K:GLU139
|
4.8
|
73.8
|
1.0
|
C
|
K:ILE141
|
4.9
|
75.5
|
1.0
|
N
|
K:ILE141
|
5.0
|
75.7
|
1.0
|
|
Reference:
K.V.Korotkov,
W.G.Hol.
Structure of the Gspk-Gspi-Gspj Complex From the Enterotoxigenic Escherichia Coli Type 2 Secretion System. Nat.Struct.Mol.Biol. V. 15 462 2008.
ISSN: ISSN 1545-9993
PubMed: 18438417
DOI: 10.1038/NSMB.1426
Page generated: Sat Jul 13 08:38:54 2024
|