Calcium in PDB 3dc0: Crystal Structure of Native Alpha-Amylase From Bacillus Sp. Kr-8104

All present enzymatic activity of Crystal Structure of Native Alpha-Amylase From Bacillus Sp. Kr-8104:
3.2.1.1;

The structure of Crystal Structure of Native Alpha-Amylase From Bacillus Sp. Kr-8104, PDB code: 3dc0 was solved by J.Alikhajeh, K.Khajeh, B.Ranjbar, M.Naderi-Manesh, H.Naderi-Manesh, C.J.Chen, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	30.00 / 2.78
Space group	C 2 2 21
Cell size a, b, c (Å), α, β, γ (°)	140.176, 163.141, 73.989, 90.00, 90.00, 90.00
R / Rfree (%)	19.6 / 23.3

The binding sites of Calcium atom in the Crystal Structure of Native Alpha-Amylase From Bacillus Sp. Kr-8104 (pdb code 3dc0). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 3 binding sites of Calcium where determined in the Crystal Structure of Native Alpha-Amylase From Bacillus Sp. Kr-8104, PDB code: 3dc0:
Jump to Calcium binding site number: 1; 2; 3;

Calcium binding site 1 out of 3 in the Crystal Structure of Native Alpha-Amylase From Bacillus Sp. Kr-8104


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Crystal Structure of Native Alpha-Amylase From Bacillus Sp. Kr-8104 within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca1001 b:22.9 occ:1.00 O A:GLY169 2.7 16.3 1.0 OD1 A:ASP171 2.8 31.6 1.0 OD2 A:ASP171 2.8 29.6 1.0 CG A:ASP171 3.2 28.5 1.0 OE1 A:GLU88 3.5 25.8 1.0 C A:GLY169 3.6 16.0 1.0 CA A:GLY169 4.0 16.5 1.0 CD A:GLU88 4.4 27.0 1.0 CG A:GLU88 4.6 24.0 1.0 CB A:GLU88 4.6 21.4 1.0 O A:CYS84 4.6 11.8 1.0 CB A:ASP171 4.7 26.1 1.0 N A:ALA170 4.8 5.1 1.0 C A:ALA170 4.9 3.5 1.0 CB A:CYS84 5.0 9.2 1.0

Calcium binding site 2 out of 3 in the Crystal Structure of Native Alpha-Amylase From Bacillus Sp. Kr-8104


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of Crystal Structure of Native Alpha-Amylase From Bacillus Sp. Kr-8104 within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca1002 b:22.9 occ:1.00 O A:GLY313 2.6 18.1 1.0 OE2 A:GLU276 2.7 10.2 1.0 OE1 A:GLU276 2.9 18.1 1.0 CD A:GLU276 3.2 14.0 1.0 C A:GLY313 3.6 16.3 1.0 CA A:VAL314 4.3 8.9 1.0 N A:VAL314 4.3 9.2 1.0 CA A:GLY313 4.5 16.1 1.0 CG A:GLU276 4.7 11.1 1.0 N A:ARG315 4.8 10.1 1.0

Calcium binding site 3 out of 3 in the Crystal Structure of Native Alpha-Amylase From Bacillus Sp. Kr-8104


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 3 of Crystal Structure of Native Alpha-Amylase From Bacillus Sp. Kr-8104 within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca1004 b:22.9 occ:1.00 O A:HIS180 2.5 18.0 1.0 OD2 A:ASP146 2.6 19.5 1.0 OD1 A:ASN101 2.7 11.6 1.0 O A:THR137 2.7 25.6 1.0 OD1 A:ASP146 2.8 25.8 1.0 O A:HOH1006 3.0 17.6 1.0 CG A:ASP146 3.1 22.7 1.0 C A:THR137 3.4 22.3 1.0 C A:HIS180 3.6 17.1 1.0 CG A:ASN101 3.7 9.0 1.0 CA A:THR137 3.8 21.6 1.0 CB A:HIS180 3.9 19.8 1.0 ND2 A:ASN101 4.0 5.8 1.0 O A:ASN139 4.1 22.4 1.0 O A:VAL136 4.2 19.6 1.0 N A:GLN138 4.3 18.2 1.0 CA A:HIS180 4.3 16.0 1.0 N A:ILE181 4.5 17.1 1.0 CB A:ASP146 4.5 19.7 1.0 CD1 A:ILE181 4.6 27.0 1.0 O A:ASN101 4.7 11.3 1.0 CA A:GLN138 4.7 17.6 1.0 CB A:THR137 4.7 9.9 1.0 CA A:ILE181 4.7 18.2 1.0 ND1 A:HIS122 4.7 19.4 1.0 N A:THR137 4.8 21.5 1.0 C A:GLN138 4.9 18.0 1.0 N A:ASN139 4.9 21.3 1.0 C A:VAL136 4.9 20.7 1.0

J.Alikhajeh, K.Khajeh, B.Ranjbar, M.Naderi-Manesh, H.Naderi-Manesh, C.J.Chen. Crystal Structure of Native Alpha-Amylase From Bacillus Sp. Kr-8104 To Be Published.