Calcium in PDB 3e5s: Crystal Structure of Staphylococcal Nuclease Variant Delta+Phs L103K at Cryogenic Temperature

All present enzymatic activity of Crystal Structure of Staphylococcal Nuclease Variant Delta+Phs L103K at Cryogenic Temperature:
3.1.31.1;

The structure of Crystal Structure of Staphylococcal Nuclease Variant Delta+Phs L103K at Cryogenic Temperature, PDB code: 3e5s was solved by V.S.Khangulov, J.L.Schlessman, E.B.Garcia-Moreno, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	N/A / 2.00
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	31.172, 78.514, 60.949, 90.00, 90.04, 90.00
R / Rfree (%)	16.4 / 22.4

The binding sites of Calcium atom in the Crystal Structure of Staphylococcal Nuclease Variant Delta+Phs L103K at Cryogenic Temperature (pdb code 3e5s). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 2 binding sites of Calcium where determined in the Crystal Structure of Staphylococcal Nuclease Variant Delta+Phs L103K at Cryogenic Temperature, PDB code: 3e5s:
Jump to Calcium binding site number: 1; 2;

Calcium binding site 1 out of 2 in the Crystal Structure of Staphylococcal Nuclease Variant Delta+Phs L103K at Cryogenic Temperature


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Crystal Structure of Staphylococcal Nuclease Variant Delta+Phs L103K at Cryogenic Temperature within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca151 b:34.3 occ:1.00 O6P A:THP150 2.7 14.6 1.0 O A:HOH224 2.7 35.7 1.0 OD2 A:ASP21 2.8 15.9 1.0 O A:HOH208 2.9 13.2 1.0 O A:HOH156 2.9 14.2 1.0 O A:HOH209 3.1 20.0 1.0 OD1 A:ASP40 3.2 13.4 1.0 OE2 A:GLU43 3.6 41.4 1.0 O A:HOH160 3.6 11.3 1.0 NH2 A:ARG35 3.6 8.5 1.0 P2 A:THP150 3.6 12.0 1.0 O4P A:THP150 3.7 6.3 1.0 CG A:ASP21 3.9 13.4 1.0 O A:THR41 4.0 11.7 1.0 O5P A:THP150 4.1 5.5 1.0 N A:THR41 4.3 6.8 1.0 CB A:ASP21 4.3 9.7 1.0 CZ A:ARG35 4.4 9.3 1.0 CG A:ASP40 4.4 14.8 1.0 NE A:ARG35 4.6 6.9 1.0 CD A:GLU43 4.6 26.8 1.0 CA A:ASP40 4.7 6.2 1.0 OD1 A:ASP21 4.9 10.2 1.0 C A:THR41 4.9 14.0 1.0 C A:ASP40 5.0 8.7 1.0

Calcium binding site 2 out of 2 in the Crystal Structure of Staphylococcal Nuclease Variant Delta+Phs L103K at Cryogenic Temperature


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of Crystal Structure of Staphylococcal Nuclease Variant Delta+Phs L103K at Cryogenic Temperature within 5.0Å range: probe atom residue distance (Å) B Occ B:Ca151 b:11.0 occ:0.50 O B:HOH167 2.6 18.3 1.0 OD2 B:ASP21 2.7 7.1 1.0 O B:HOH155 2.7 12.0 1.0 O6P B:THP150 2.8 9.9 1.0 OD1 B:ASP40 3.1 18.4 1.0 O B:HOH183 3.2 19.8 1.0 O5P B:THP150 3.6 10.2 1.0 P2 B:THP150 3.7 9.9 1.0 CG B:ASP21 3.7 10.9 1.0 NH2 B:ARG35 3.8 11.1 1.0 O B:HOH159 3.8 12.2 1.0 O B:THR41 4.1 6.8 1.0 CB B:ASP21 4.1 7.4 1.0 O4P B:THP150 4.2 8.7 1.0 CG B:ASP40 4.3 18.4 1.0 N B:THR41 4.3 11.0 1.0 CZ B:ARG35 4.4 5.3 1.0 NE B:ARG35 4.6 9.3 1.0 OE1 B:GLU43 4.8 27.1 1.0 OD1 B:ASP21 4.8 7.1 1.0 O B:HOH226 4.8 18.1 1.0 OE2 B:GLU43 4.9 26.2 1.0 CA B:ASP40 4.9 11.4 1.0

V.S.Khangulov, J.L.Schlessman, E.B.Garcia-Moreno. Crystal Structure of Staphylococcal Nuclease Variant Delta+Phs L103K at Cryogenic Temperature To Be Published.