Calcium in PDB 3ehu: Crystal Structure of the Extracellular Domain of Human Corticotropin Releasing Factor Receptor Type 1 (CRFR1) in Complex with Crf

The structure of Crystal Structure of the Extracellular Domain of Human Corticotropin Releasing Factor Receptor Type 1 (CRFR1) in Complex with Crf, PDB code: 3ehu was solved by A.A.Pioszak, H.E.Xu, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	39.50 / 1.96
Space group	P 1
Cell size a, b, c (Å), α, β, γ (°)	49.203, 63.480, 85.875, 99.75, 106.28, 101.67
R / Rfree (%)	20.9 / 25.6

The binding sites of Calcium atom in the Crystal Structure of the Extracellular Domain of Human Corticotropin Releasing Factor Receptor Type 1 (CRFR1) in Complex with Crf (pdb code 3ehu). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 2 binding sites of Calcium where determined in the Crystal Structure of the Extracellular Domain of Human Corticotropin Releasing Factor Receptor Type 1 (CRFR1) in Complex with Crf, PDB code: 3ehu:
Jump to Calcium binding site number: 1; 2;

Calcium binding site 1 out of 2 in the Crystal Structure of the Extracellular Domain of Human Corticotropin Releasing Factor Receptor Type 1 (CRFR1) in Complex with Crf


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Crystal Structure of the Extracellular Domain of Human Corticotropin Releasing Factor Receptor Type 1 (CRFR1) in Complex with Crf within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca500 b:18.9 occ:1.00 O A:HOH595 2.3 21.5 1.0 OD1 A:ASP-308 2.3 24.0 1.0 O1 A:BTB501 2.4 22.9 1.0 O8 A:BTB501 2.4 22.4 1.0 O6 A:BTB501 2.5 21.4 1.0 O A:HOH508 2.6 13.6 1.0 O3 A:BTB501 2.6 24.1 1.0 N A:BTB501 3.2 26.7 1.0 C2 A:BTB501 3.4 27.1 1.0 C1 A:BTB501 3.4 25.4 1.0 C8 A:BTB501 3.4 26.6 1.0 C6 A:BTB501 3.4 24.1 1.0 C3 A:BTB501 3.4 26.1 1.0 CG A:ASP-308 3.6 25.6 1.0 C7 A:BTB501 3.6 27.2 1.0 C5 A:BTB501 3.7 27.4 1.0 O A:HOH713 3.9 52.9 1.0 O B:HOH542 4.3 19.3 1.0 OD2 A:ASP-308 4.3 24.5 1.0 N A:LYS-307 4.3 26.6 1.0 CA A:ASP-308 4.6 26.3 1.0 OD1 A:ASN-337 4.6 27.8 1.0 CB A:ASP-308 4.6 25.9 1.0 O A:HOH708 4.6 34.0 1.0 ND2 A:ASN-337 4.6 26.5 1.0 O A:HOH603 4.9 31.2 1.0 C4 A:BTB501 4.9 28.8 1.0 O A:HOH506 4.9 10.7 1.0

Calcium binding site 2 out of 2 in the Crystal Structure of the Extracellular Domain of Human Corticotropin Releasing Factor Receptor Type 1 (CRFR1) in Complex with Crf


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of Crystal Structure of the Extracellular Domain of Human Corticotropin Releasing Factor Receptor Type 1 (CRFR1) in Complex with Crf within 5.0Å range: probe atom residue distance (Å) B Occ B:Ca500 b:17.1 occ:1.00 O A:HOH515 2.3 15.7 1.0 O6 B:BTB501 2.4 17.8 1.0 OD1 B:ASP-308 2.4 27.1 1.0 O8 B:BTB501 2.4 14.4 1.0 O A:HOH514 2.5 15.5 1.0 O1 B:BTB501 2.5 22.0 1.0 O3 B:BTB501 2.5 21.4 1.0 N B:BTB501 3.1 23.0 1.0 C6 B:BTB501 3.3 20.4 1.0 C2 B:BTB501 3.4 24.0 1.0 C1 B:BTB501 3.4 23.6 1.0 C3 B:BTB501 3.5 23.6 1.0 C8 B:BTB501 3.5 20.3 1.0 C7 B:BTB501 3.6 23.2 1.0 CG B:ASP-308 3.6 27.2 1.0 C5 B:BTB501 3.7 22.2 1.0 O B:HOH618 4.0 41.4 1.0 O B:HOH567 4.3 22.7 1.0 N B:LYS-307 4.3 26.3 1.0 OD2 B:ASP-308 4.4 24.1 1.0 CA B:ASP-308 4.6 26.1 1.0 ND2 B:ASN-337 4.6 28.1 1.0 CB B:ASP-308 4.6 25.5 1.0 OD1 B:ASN-337 4.7 29.7 1.0 O A:HOH507 4.7 12.4 1.0 C4 B:BTB501 4.9 25.8 1.0

A.A.Pioszak, N.R.Parker, K.Suino-Powell, H.E.Xu. Molecular Recognition of Corticotropin-Releasing Factor By Its G-Protein-Coupled Receptor CRFR1. J.Biol.Chem. V. 283 32900 2008.
ISSN: ISSN 0021-9258
PubMed: 18801728
DOI: 10.1074/JBC.M805749200