Calcium in PDB 3evv: Crystal Structure of Calcium Bound Dimeric GCAMP2 (#2)

The structure of Crystal Structure of Calcium Bound Dimeric GCAMP2 (#2), PDB code: 3evv was solved by Q.Wang, B.Shui, M.I.Kotlikoff, H.Sondermann, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	33.40 / 2.60
Space group	C 1 2 1
Cell size a, b, c (Å), α, β, γ (°)	127.476, 46.955, 67.849, 90.00, 100.09, 90.00
R / Rfree (%)	21.5 / 27

The binding sites of Calcium atom in the Crystal Structure of Calcium Bound Dimeric GCAMP2 (#2) (pdb code 3evv). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 4 binding sites of Calcium where determined in the Crystal Structure of Calcium Bound Dimeric GCAMP2 (#2), PDB code: 3evv:
Jump to Calcium binding site number: 1; 2; 3; 4;

Calcium binding site 1 out of 4 in the Crystal Structure of Calcium Bound Dimeric GCAMP2 (#2)


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Crystal Structure of Calcium Bound Dimeric GCAMP2 (#2) within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca505 b:26.1 occ:1.00 OE1 A:GLU443 2.6 26.0 1.0 OD1 A:ASP434 2.7 36.3 1.0 OD1 A:ASP436 2.7 38.5 1.0 O A:GLN438 2.8 39.1 1.0 OD1 A:ASP432 2.8 17.6 1.0 O A:HOH500 2.9 32.6 1.0 OE2 A:GLU443 3.1 45.8 1.0 CD A:GLU443 3.2 34.1 1.0 CG A:ASP434 3.3 37.2 1.0 CG A:ASP436 3.4 29.0 1.0 C A:GLN438 3.4 33.4 1.0 OD2 A:ASP434 3.4 40.1 1.0 OD2 A:ASP436 3.6 35.5 1.0 CG A:ASP432 3.7 23.1 1.0 CA A:VAL439 4.0 8.8 1.0 N A:VAL439 4.0 18.6 1.0 CA A:ASP432 4.0 12.6 1.0 N A:ASN440 4.1 20.8 1.0 N A:GLN438 4.3 28.1 1.0 CB A:ASP432 4.3 27.5 1.0 N A:ILE433 4.3 24.9 1.0 CA A:GLN438 4.3 31.2 1.0 N A:ASP436 4.4 19.9 1.0 N A:ASP434 4.4 13.6 1.0 C A:ASP432 4.5 11.9 1.0 CB A:ASP436 4.5 24.3 1.0 C A:VAL439 4.5 14.2 1.0 CB A:GLN438 4.6 30.1 1.0 OD2 A:ASP432 4.6 9.5 1.0 N A:GLY435 4.6 14.6 1.0 CG A:GLU443 4.6 27.9 1.0 CB A:ASP434 4.7 31.3 1.0 CG A:ASN440 4.8 24.3 1.0 OD1 A:ASN440 4.8 38.0 1.0 CA A:ASP436 4.9 22.0 1.0 CA A:ASP434 5.0 27.5 1.0 ND2 A:ASN440 5.0 24.4 1.0

Calcium binding site 2 out of 4 in the Crystal Structure of Calcium Bound Dimeric GCAMP2 (#2)


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of Crystal Structure of Calcium Bound Dimeric GCAMP2 (#2) within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca506 b:64.5 occ:1.00 OE2 A:GLU370 2.8 31.6 1.0 O A:THR365 3.0 42.6 1.0 OD1 A:ASP359 3.1 43.5 1.0 ND2 A:ASN363 3.1 50.4 1.0 OE1 A:GLU370 3.3 48.1 1.0 CD A:GLU370 3.3 39.9 1.0 OD2 A:ASP367 3.4 63.2 1.0 CG A:ASP359 3.4 33.0 1.0 CB A:ASN363 3.4 36.0 1.0 OD1 A:ASP361 3.5 61.9 1.0 CG A:ASN363 3.5 38.5 1.0 CG A:ASP367 3.7 60.2 1.0 OD2 A:ASP359 3.7 39.7 1.0 OD1 A:ASP367 3.8 76.6 1.0 N A:ASN363 3.9 37.6 1.0 C A:THR365 4.0 47.1 1.0 CG A:ASP361 4.0 61.5 1.0 OD2 A:ASP361 4.0 73.8 1.0 N A:GLY364 4.0 27.2 1.0 CA A:ASN363 4.2 39.9 1.0 CB A:ASP359 4.3 23.4 1.0 N A:ASP367 4.3 33.9 1.0 N A:THR365 4.4 47.9 1.0 OD1 A:ASN363 4.4 40.8 1.0 CB A:ASP367 4.6 37.8 1.0 C A:ASN363 4.6 49.0 1.0 CG A:GLU370 4.7 31.9 1.0 CA A:ASP359 4.7 22.2 1.0 CA A:ILE366 4.7 35.6 1.0 N A:ILE366 4.7 42.3 1.0 CA A:THR365 4.8 52.1 1.0 C A:GLY364 4.8 45.9 1.0 N A:GLY362 4.9 38.6 1.0 C A:ILE366 4.9 31.8 1.0 OG1 A:THR365 5.0 62.2 1.0

Calcium binding site 3 out of 4 in the Crystal Structure of Calcium Bound Dimeric GCAMP2 (#2)


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 3 of Crystal Structure of Calcium Bound Dimeric GCAMP2 (#2) within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca507 b:50.3 occ:1.00 O A:TYR402 2.6 30.1 1.0 O A:HOH501 2.7 45.3 1.0 OD1 A:ASP398 2.8 59.1 1.0 OD1 A:ASN400 2.8 35.4 1.0 OE2 A:GLU407 2.8 62.0 1.0 OD2 A:ASP398 3.0 71.2 1.0 OD1 A:ASP396 3.1 64.0 1.0 OE1 A:GLU407 3.2 51.3 1.0 CG A:ASN400 3.2 29.8 1.0 CG A:ASP398 3.3 60.1 1.0 CD A:GLU407 3.3 52.1 1.0 ND2 A:ASN400 3.3 16.9 1.0 C A:TYR402 3.6 19.0 1.0 CG A:ASP396 4.0 55.7 1.0 N A:TYR402 4.2 20.6 1.0 CA A:TYR402 4.3 17.2 1.0 CB A:ASN400 4.3 32.0 1.0 OD2 A:ASP396 4.4 52.4 1.0 N A:ASN400 4.4 41.1 1.0 CB A:TYR402 4.5 18.6 1.0 N A:ILE403 4.7 12.1 1.0 CG A:GLU407 4.7 37.3 1.0 CB A:ASP398 4.8 47.4 1.0 CA A:ASN400 4.8 39.8 1.0 CA A:ILE403 4.8 12.9 1.0 NE2 A:GLN438 4.8 47.9 1.0 N A:SER404 4.9 18.8 1.0 N A:GLY401 4.9 39.4 1.0

Calcium binding site 4 out of 4 in the Crystal Structure of Calcium Bound Dimeric GCAMP2 (#2)


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 4 of Crystal Structure of Calcium Bound Dimeric GCAMP2 (#2) within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca508 b:54.1 occ:1.00 OD1 A:ASP325 2.5 33.8 1.0 O A:THR329 2.6 36.9 1.0 OD1 A:ASP327 2.7 35.1 1.0 OE1 A:GLU334 2.7 43.0 1.0 OE2 A:GLU334 2.7 57.5 1.0 CD A:GLU334 3.0 47.3 1.0 OD1 A:ASP323 3.1 52.3 1.0 CG A:ASP327 3.1 26.8 1.0 OD2 A:ASP327 3.2 30.5 1.0 CG A:ASP325 3.4 39.2 1.0 OD2 A:ASP325 3.5 38.4 1.0 C A:THR329 3.6 23.4 1.0 OG1 A:THR329 3.6 23.3 1.0 CG A:ASP323 3.9 43.1 1.0 N A:THR329 4.1 26.9 1.0 CB A:ASP327 4.3 16.6 1.0 CA A:THR329 4.3 24.3 1.0 N A:ASP327 4.4 24.2 1.0 CA A:ASP323 4.4 37.0 1.0 CG A:GLU334 4.5 45.7 1.0 N A:ILE330 4.5 22.4 1.0 CB A:ASP323 4.6 36.7 1.0 CB A:THR329 4.6 36.1 1.0 OD2 A:ASP323 4.7 37.2 1.0 O A:HOH502 4.7 31.9 1.0 CA A:ILE330 4.7 19.6 1.0 N A:ASP325 4.7 35.0 1.0 CB A:ASP325 4.8 32.2 1.0 CA A:ASP327 4.8 24.2 1.0 N A:THR331 4.9 21.3 1.0 N A:GLY328 4.9 19.9 1.0 N A:LYS324 4.9 34.8 1.0 C A:ASP323 4.9 47.2 1.0

Q.Wang, B.Shui, M.I.Kotlikoff, H.Sondermann. Structural Basis For Calcium Sensing By GCAMP2. Structure V. 16 1817 2008.
ISSN: ISSN 0969-2126
PubMed: 19081058
DOI: 10.1016/J.STR.2008.10.008