Calcium in PDB 3f1v: E. Coli Beta Sliding Clamp, 148-153 Ala Mutant

All present enzymatic activity of E. Coli Beta Sliding Clamp, 148-153 Ala Mutant:
2.7.7.7;

The structure of E. Coli Beta Sliding Clamp, 148-153 Ala Mutant, PDB code: 3f1v was solved by V.Cody, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	28.86 / 1.77
Space group	P 1
Cell size a, b, c (Å), α, β, γ (°)	40.838, 64.471, 72.111, 73.83, 82.72, 83.76
R / Rfree (%)	21.5 / 28.2

The structure of E. Coli Beta Sliding Clamp, 148-153 Ala Mutant also contains other interesting chemical elements:

Chlorine

(Cl)

1 atom

The binding sites of Calcium atom in the E. Coli Beta Sliding Clamp, 148-153 Ala Mutant (pdb code 3f1v). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 2 binding sites of Calcium where determined in the E. Coli Beta Sliding Clamp, 148-153 Ala Mutant, PDB code: 3f1v:
Jump to Calcium binding site number: 1; 2;

Calcium binding site 1 out of 2 in the E. Coli Beta Sliding Clamp, 148-153 Ala Mutant


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of E. Coli Beta Sliding Clamp, 148-153 Ala Mutant within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca367 b:30.4 occ:1.00 O A:HOH368 2.2 26.4 1.0 O A:LEU119 2.2 47.5 1.0 O A:HOH370 2.3 35.0 1.0 O A:HOH371 2.4 32.7 1.0 O A:HOH375 2.6 39.1 1.0 O A:ASN118 2.8 39.3 1.0 N A:LEU119 3.3 41.4 1.0 C A:LEU119 3.3 44.8 1.0 C A:ASN118 3.4 40.3 1.0 CA A:LEU119 3.8 44.5 1.0 CB A:LEU119 4.3 45.0 1.0 O A:HOH443 4.3 37.7 1.0 O A:HOH439 4.3 30.3 1.0 O A:HOH487 4.4 53.2 1.0 N A:ASP120 4.5 45.9 1.0 O A:HOH459 4.6 37.0 1.0 OD1 A:ASP121 4.6 37.1 1.0 OE1 A:GLU52 4.6 37.4 1.0 OE2 A:GLU52 4.8 37.7 1.0 C A:ASP120 4.8 44.3 1.0 CA A:ASN118 4.8 38.8 1.0 O A:ASP120 4.8 47.1 1.0 CA A:ASP120 4.8 45.6 1.0

Calcium binding site 2 out of 2 in the E. Coli Beta Sliding Clamp, 148-153 Ala Mutant


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of E. Coli Beta Sliding Clamp, 148-153 Ala Mutant within 5.0Å range: probe atom residue distance (Å) B Occ B:Ca367 b:29.6 occ:1.00 ND2 B:ASN118 2.3 28.5 1.0 O B:HOH373 2.3 30.7 1.0 O B:HOH372 2.3 42.5 1.0 O B:HOH369 2.4 30.8 1.0 O B:LEU119 2.4 28.9 1.0 O B:HOH374 2.5 35.7 1.0 O B:HOH380 2.6 38.3 1.0 O B:HOH434 2.9 41.3 1.0 CG B:ASN118 3.5 30.1 1.0 C B:LEU119 3.6 29.1 1.0 N B:LEU119 3.8 29.7 1.0 OD1 B:ASN118 4.1 38.6 1.0 C B:ASN118 4.1 30.1 1.0 CA B:LEU119 4.2 29.4 1.0 O B:HOH466 4.2 35.0 1.0 OD1 B:ASP121 4.3 33.6 1.0 O B:HOH433 4.4 36.5 1.0 O B:HOH447 4.4 32.4 1.0 O B:HOH552 4.5 47.0 1.0 O B:ASN118 4.6 31.4 1.0 CB B:LEU119 4.6 30.3 1.0 CA B:ASN118 4.6 30.7 1.0 CB B:ASN118 4.6 30.0 1.0 C B:ASP120 4.7 30.1 1.0 N B:ASP120 4.7 28.8 1.0 OE1 B:GLU52 4.7 36.0 1.0 O B:HOH508 4.7 40.4 1.0 OE2 B:GLU52 4.7 36.9 1.0 O B:HOH454 4.8 42.0 1.0 N B:ASP121 4.8 30.0 1.0 CA B:ASP120 4.9 30.2 1.0 O B:ASP120 4.9 28.9 1.0

J.M.Heltzel, S.K.Scouten Ponticelli, L.H.Sanders, J.M.Duzen, V.Cody, J.Pace, E.H.Snell, M.D.Sutton. Sliding Clamp-Dna Interactions Are Required For Viability and Contribute to Dna Polymerase Management in Escherichia Coli. J.Mol.Biol. V. 387 74 2009.
ISSN: ISSN 0022-2836
PubMed: 19361435
DOI: 10.1016/J.JMB.2009.01.050