Calcium in PDB 3g5c: Structural and Biochemical Studies on the Ectodomain of Human ADAM22

The structure of Structural and Biochemical Studies on the Ectodomain of Human ADAM22, PDB code: 3g5c was solved by H.Liu, A.Shim, X.Chen, X.He, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	50.00 / 2.36
Space group	H 3
Cell size a, b, c (Å), α, β, γ (°)	122.090, 122.090, 208.690, 90.00, 90.00, 120.00
R / Rfree (%)	24.5 / 27.3

The binding sites of Calcium atom in the Structural and Biochemical Studies on the Ectodomain of Human ADAM22 (pdb code 3g5c). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 6 binding sites of Calcium where determined in the Structural and Biochemical Studies on the Ectodomain of Human ADAM22, PDB code: 3g5c:
Jump to Calcium binding site number: 1; 2; 3; 4; 5; 6;

Calcium binding site 1 out of 6 in the Structural and Biochemical Studies on the Ectodomain of Human ADAM22


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Structural and Biochemical Studies on the Ectodomain of Human ADAM22 within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca801 b:48.9 occ:1.00 O A:HOH800 2.2 92.6 1.0 O A:CYS433 2.2 32.1 1.0 O A:HOH776 2.3 74.2 1.0 OD1 A:ASN436 2.4 43.7 1.0 OD2 A:ASP325 2.5 43.7 1.0 OE2 A:GLU242 2.7 54.1 1.0 OD1 A:ASP325 2.7 41.5 1.0 CG A:ASP325 2.9 38.9 1.0 CD A:GLU242 3.3 45.0 1.0 CG A:ASN436 3.3 36.2 1.0 C A:CYS433 3.4 30.8 1.0 ND2 A:ASN436 3.5 32.0 1.0 OE1 A:GLU242 3.9 50.8 1.0 CG A:GLU242 4.0 41.2 1.0 CA A:CYS433 4.2 30.1 1.0 CB A:CYS433 4.2 43.1 1.0 CB A:GLU242 4.3 42.2 1.0 O A:TYR240 4.3 45.8 1.0 N A:LEU434 4.4 36.6 1.0 CB A:ASP325 4.5 38.2 1.0 O A:ASN436 4.6 52.2 1.0 CA A:LEU434 4.6 38.9 1.0 CB A:ASN436 4.7 43.8 1.0 N A:ASN436 4.8 47.1 1.0 N A:GLU242 4.9 31.3 1.0 SG A:CYS433 4.9 46.0 1.0

Calcium binding site 2 out of 6 in the Structural and Biochemical Studies on the Ectodomain of Human ADAM22


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of Structural and Biochemical Studies on the Ectodomain of Human ADAM22 within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca802 b:78.4 occ:1.00 OD2 A:ASP459 2.2 64.6 1.0 O A:GLU446 2.3 98.4 1.0 OD1 A:ASN449 2.4 56.9 1.0 OE1 A:GLU453 2.5 79.6 1.0 OE1 A:GLU456 2.7 0.9 1.0 OE2 A:GLU456 2.8 95.9 1.0 O A:PHE451 2.9 57.2 1.0 CD A:GLU456 3.1 98.3 1.0 CG A:ASN449 3.2 59.3 1.0 C A:GLU446 3.2 0.0 1.0 CG A:ASP459 3.5 80.6 1.0 CA A:CYS447 3.5 78.3 1.0 ND2 A:ASN449 3.5 57.2 1.0 N A:GLY448 3.6 91.8 1.0 N A:CYS447 3.7 78.3 1.0 CD A:GLU453 3.7 80.7 1.0 N A:ASN449 3.9 85.0 1.0 C A:CYS447 4.0 75.7 1.0 C A:PHE451 4.1 55.0 1.0 OD1 A:ASP459 4.2 72.4 1.0 N A:GLU446 4.2 97.8 1.0 CA A:GLU446 4.3 99.7 1.0 CB A:ASN449 4.4 64.2 1.0 N A:PHE451 4.4 54.5 1.0 CG A:GLU453 4.5 76.2 1.0 CB A:ASP459 4.5 83.6 1.0 OE2 A:GLU446 4.6 0.8 1.0 CG A:GLU456 4.6 99.3 1.0 OE2 A:GLU453 4.6 77.8 1.0 SG A:CYS477 4.6 92.4 1.0 CA A:ASN449 4.6 80.6 1.0 N A:GLY450 4.7 83.1 1.0 CA A:GLY448 4.7 90.6 1.0 CA A:PHE451 4.7 53.7 1.0 CB A:CYS447 4.8 0.7 1.0 C A:GLY448 4.8 87.7 1.0 N A:GLU453 4.9 91.2 1.0 CB A:GLU453 5.0 75.5 1.0

Calcium binding site 3 out of 6 in the Structural and Biochemical Studies on the Ectodomain of Human ADAM22


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 3 of Structural and Biochemical Studies on the Ectodomain of Human ADAM22 within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca803 b:47.5 occ:1.00 O A:ILE512 2.4 27.2 1.0 OD1 A:ASN526 2.5 31.6 1.0 OD2 A:ASP511 2.5 31.5 1.0 O A:ILE527 2.6 38.5 1.0 O A:HOH109 2.6 61.9 1.0 OE2 A:GLU514 2.7 29.1 1.0 OE1 A:GLU514 2.7 52.9 1.0 CD A:GLU514 2.8 47.8 1.0 CG A:ASP511 3.2 26.9 1.0 OD1 A:ASP511 3.3 26.0 1.0 CG A:ASN526 3.5 40.6 1.0 C A:ILE512 3.5 28.8 1.0 C A:ILE527 3.6 40.8 1.0 N A:ILE512 3.8 27.3 1.0 N A:ILE527 3.8 41.1 1.0 CG A:GLU514 4.0 50.0 1.0 ND2 A:ASN526 4.1 42.8 1.0 CA A:ILE512 4.2 26.9 1.0 O A:HOH192 4.3 62.7 1.0 CA A:ILE527 4.4 38.4 1.0 C A:ASN526 4.5 40.3 1.0 CB A:ASP511 4.5 29.9 1.0 N A:ARG513 4.5 46.7 1.0 CA A:ASN526 4.5 40.6 1.0 CB A:ILE512 4.6 33.6 1.0 O A:HOH28 4.6 70.6 1.0 CB A:ASN526 4.6 37.5 1.0 N A:HIS528 4.6 38.1 1.0 C A:ASP511 4.7 40.0 1.0 CA A:ARG513 4.8 50.6 1.0 CA A:HIS528 4.8 36.8 1.0 CD A:ARG504 4.9 27.5 1.0 CA A:ASP511 4.9 39.0 1.0 C A:ARG513 4.9 51.3 1.0

Calcium binding site 4 out of 6 in the Structural and Biochemical Studies on the Ectodomain of Human ADAM22


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 4 of Structural and Biochemical Studies on the Ectodomain of Human ADAM22 within 5.0Å range: probe atom residue distance (Å) B Occ B:Ca801 b:48.7 occ:1.00 O B:CYS433 2.2 32.1 1.0 O B:HOH778 2.2 59.9 1.0 O B:HOH122 2.3 81.1 1.0 OD1 B:ASN436 2.3 43.7 1.0 OD2 B:ASP325 2.5 43.6 1.0 OE2 B:GLU242 2.6 54.0 1.0 OD1 B:ASP325 2.6 41.5 1.0 CG B:ASP325 2.9 38.8 1.0 CD B:GLU242 3.2 44.9 1.0 CG B:ASN436 3.3 36.2 1.0 C B:CYS433 3.4 30.7 1.0 ND2 B:ASN436 3.6 32.0 1.0 OE1 B:GLU242 3.9 50.7 1.0 CG B:GLU242 4.0 41.1 1.0 O B:TYR240 4.2 45.7 1.0 CA B:CYS433 4.2 30.1 1.0 CB B:GLU242 4.3 42.1 1.0 CB B:CYS433 4.3 43.1 1.0 N B:LEU434 4.4 36.6 1.0 CB B:ASP325 4.5 38.1 1.0 CA B:LEU434 4.5 39.0 1.0 O B:ASN436 4.6 52.1 1.0 CB B:ASN436 4.6 43.8 1.0 N B:ASN436 4.7 47.1 1.0 N B:GLU242 4.8 31.4 1.0 N B:PHE435 4.9 53.7 1.0 SG B:CYS433 5.0 46.0 1.0

Calcium binding site 5 out of 6 in the Structural and Biochemical Studies on the Ectodomain of Human ADAM22


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 5 of Structural and Biochemical Studies on the Ectodomain of Human ADAM22 within 5.0Å range: probe atom residue distance (Å) B Occ B:Ca802 b:78.5 occ:1.00 OD2 B:ASP459 2.2 64.6 1.0 O B:GLU446 2.3 98.4 1.0 OD1 B:ASN449 2.4 56.9 1.0 OE1 B:GLU453 2.6 79.6 1.0 OE2 B:GLU456 2.9 95.9 1.0 O B:PHE451 2.9 57.3 1.0 OE1 B:GLU456 3.0 0.9 1.0 CG B:ASN449 3.0 59.3 1.0 ND2 B:ASN449 3.3 57.2 1.0 CD B:GLU456 3.3 98.3 1.0 C B:GLU446 3.3 0.0 1.0 CG B:ASP459 3.4 80.7 1.0 N B:GLY448 3.5 91.8 1.0 CA B:CYS447 3.7 78.3 1.0 N B:ASN449 3.7 85.0 1.0 CD B:GLU453 3.8 80.7 1.0 N B:CYS447 3.9 78.3 1.0 C B:PHE451 4.0 55.2 1.0 C B:CYS447 4.1 75.7 1.0 CB B:ASN449 4.1 64.2 1.0 OD1 B:ASP459 4.2 72.5 1.0 N B:PHE451 4.2 54.6 1.0 CB B:ASP459 4.4 83.6 1.0 N B:GLY450 4.4 83.2 1.0 CA B:ASN449 4.4 80.6 1.0 N B:GLU446 4.4 97.8 1.0 CA B:GLU446 4.5 99.7 1.0 OE2 B:GLU446 4.5 0.8 1.0 CA B:PHE451 4.6 53.9 1.0 CA B:GLY448 4.6 90.6 1.0 OE2 B:GLU453 4.6 77.9 1.0 CG B:GLU453 4.6 76.2 1.0 C B:GLY448 4.6 87.7 1.0 SG B:CYS477 4.7 92.4 1.0 CG B:GLU456 4.8 99.4 1.0 C B:ASN449 4.8 78.4 1.0 CB B:CYS447 4.9 0.7 1.0 CB B:PHE451 4.9 72.0 1.0 N B:GLU453 5.0 91.2 1.0

Calcium binding site 6 out of 6 in the Structural and Biochemical Studies on the Ectodomain of Human ADAM22


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 6 of Structural and Biochemical Studies on the Ectodomain of Human ADAM22 within 5.0Å range: probe atom residue distance (Å) B Occ B:Ca803 b:47.5 occ:1.00 O B:ILE512 2.5 27.2 1.0 OD1 B:ASN526 2.5 31.6 1.0 OE1 B:GLU514 2.6 53.0 1.0 OD2 B:ASP511 2.6 31.4 1.0 O B:ILE527 2.6 38.5 1.0 O B:HOH82 2.7 37.5 1.0 CD B:GLU514 2.8 48.0 1.0 OE2 B:GLU514 2.8 29.2 1.0 CG B:ASN526 3.3 40.6 1.0 CG B:ASP511 3.3 26.8 1.0 OD1 B:ASP511 3.5 25.8 1.0 C B:ILE512 3.6 28.9 1.0 C B:ILE527 3.7 40.7 1.0 N B:ILE527 3.7 41.1 1.0 ND2 B:ASN526 3.8 42.8 1.0 CG B:GLU514 3.9 50.1 1.0 N B:ILE512 4.0 27.3 1.0 O B:HOH147 4.2 76.2 1.0 CA B:ILE527 4.3 38.4 1.0 C B:ASN526 4.3 40.3 1.0 CA B:ILE512 4.4 27.0 1.0 CA B:ASN526 4.4 40.6 1.0 CB B:ASN526 4.4 37.5 1.0 O B:HOH31 4.5 63.2 1.0 N B:ARG513 4.6 46.7 1.0 N B:HIS528 4.7 38.0 1.0 CB B:ASP511 4.7 29.7 1.0 CD B:ARG504 4.7 27.5 1.0 CB B:ILE512 4.8 33.5 1.0 CA B:ARG513 4.8 50.5 1.0 C B:ARG513 4.9 51.3 1.0 CA B:HIS528 4.9 36.6 1.0 C B:ASP511 4.9 40.0 1.0 NH1 B:ARG504 5.0 46.8 1.0 N B:GLU514 5.0 41.6 1.0

H.Liu, A.Shim, X.Chen, X.He. Structural and Biochemical Studies on the Ectodomain of Human ADAM22 J.Biol.Chem. 2009.
ISSN: ESSN 1083-351X