Calcium in PDB 3gwz: Structure of the Mitomycin 7-O-Methyltransferase Mmcr

The structure of Structure of the Mitomycin 7-O-Methyltransferase Mmcr, PDB code: 3gwz was solved by S.Singh, A.Chang, C.A.Bingman, G.N.Phillips Jr., J.S.Thorson, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	19.65 / 1.91
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	88.372, 98.918, 171.119, 90.00, 90.00, 90.00
R / Rfree (%)	17.4 / 21

The binding sites of Calcium atom in the Structure of the Mitomycin 7-O-Methyltransferase Mmcr (pdb code 3gwz). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 4 binding sites of Calcium where determined in the Structure of the Mitomycin 7-O-Methyltransferase Mmcr, PDB code: 3gwz:
Jump to Calcium binding site number: 1; 2; 3; 4;

Calcium binding site 1 out of 4 in the Structure of the Mitomycin 7-O-Methyltransferase Mmcr


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Structure of the Mitomycin 7-O-Methyltransferase Mmcr within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca350 b:36.9 occ:1.00 OD2 D:ASP87 2.6 20.2 1.0 O A:HOH855 2.6 32.6 1.0 O A:HOH417 2.6 30.5 1.0 OD1 D:ASP87 2.7 21.1 1.0 OD2 A:ASP232 2.9 44.1 1.0 O D:HOH1312 2.9 41.1 1.0 CG D:ASP87 3.0 20.3 1.0 OD1 A:ASP232 3.0 37.0 1.0 CG A:ASP232 3.3 35.7 1.0 O A:HOH428 4.0 26.4 1.0 CD A:ARG233 4.3 17.8 1.0 CG A:ARG233 4.3 23.0 1.0 CB D:ASP87 4.4 17.5 1.0 O A:GLY229 4.4 20.4 1.0 ND1 D:HIS86 4.7 21.1 1.0 O D:HOH747 4.7 28.1 1.0 O A:HOH697 4.8 28.4 1.0 CB A:ASP232 4.8 25.2 1.0 O D:HOH403 4.9 29.1 1.0

Calcium binding site 2 out of 4 in the Structure of the Mitomycin 7-O-Methyltransferase Mmcr


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of Structure of the Mitomycin 7-O-Methyltransferase Mmcr within 5.0Å range: probe atom residue distance (Å) B Occ D:Ca350 b:44.8 occ:1.00 OD2 D:ASP291 2.6 44.4 1.0 O D:ARG293 2.7 45.9 1.0 OD1 D:ASP291 2.8 37.2 1.0 O D:HOH1219 2.9 45.7 1.0 CG D:ASP291 3.0 36.4 1.0 C D:ARG293 3.3 44.4 1.0 CA D:ARG293 3.8 51.1 1.0 CB D:ARG293 3.9 48.5 1.0 N D:ARG293 3.9 42.8 1.0 N D:PRO294 4.2 39.0 1.0 CB D:ASP291 4.5 40.2 1.0 CA D:PRO294 4.5 33.6 1.0 O D:HOH772 5.0 35.4 1.0

Calcium binding site 3 out of 4 in the Structure of the Mitomycin 7-O-Methyltransferase Mmcr


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 3 of Structure of the Mitomycin 7-O-Methyltransferase Mmcr within 5.0Å range: probe atom residue distance (Å) B Occ D:Ca351 b:19.0 occ:1.00 OE2 D:GLU20 2.4 25.9 1.0 OD2 D:ASP24 2.5 17.2 1.0 O D:HOH616 2.5 19.1 1.0 O D:HOH951 2.5 19.8 1.0 OD1 D:ASP24 2.6 17.6 1.0 O D:HOH615 2.6 22.4 1.0 O D:HOH404 2.6 20.1 1.0 CG D:ASP24 2.9 15.5 1.0 CD D:GLU20 3.5 24.4 1.0 CG D:GLU20 3.8 16.8 1.0 O D:HOH422 4.2 25.1 1.0 O C:HOH614 4.3 16.8 1.0 O D:HOH923 4.3 22.1 1.0 CB D:ASP24 4.4 11.3 1.0 O D:HOH617 4.5 25.5 1.0 O C:HOH413 4.6 35.9 1.0 OE1 D:GLU20 4.6 26.6 1.0 O D:HOH954 4.6 19.8 1.0 O D:GLU20 4.9 13.7 1.0 O D:HOH613 5.0 28.3 1.0

Calcium binding site 4 out of 4 in the Structure of the Mitomycin 7-O-Methyltransferase Mmcr


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 4 of Structure of the Mitomycin 7-O-Methyltransferase Mmcr within 5.0Å range: probe atom residue distance (Å) B Occ D:Ca352 b:27.6 occ:1.00 OD2 D:ASP262 2.5 24.5 1.0 O D:HOH1322 2.5 12.1 1.0 O D:HOH386 2.6 10.7 1.0 O D:HOH1033 2.7 32.7 1.0 O D:HOH950 2.8 26.7 1.0 OE1 D:GLU134 2.9 18.3 1.0 OE2 D:GLU134 3.0 28.5 1.0 O D:HOH778 3.2 29.9 1.0 CD D:GLU134 3.2 19.5 1.0 CG D:ASP262 3.5 25.4 1.0 O D:HOH364 3.6 25.2 1.0 CB D:ASP262 3.7 20.7 1.0 O D:HOH375 4.0 17.3 1.0 O D:HOH357 4.5 16.3 1.0 O D:HOH406 4.6 19.9 1.0 O D:GLY133 4.6 13.6 1.0 OD1 D:ASP262 4.6 20.2 1.0 O D:HOH1226 4.7 31.7 1.0 CG D:GLU134 4.7 15.8 1.0 O D:HOH1261 4.8 36.2 1.0 CA D:GLU134 5.0 13.9 1.0

S.Singh, A.Chang, R.D.Goff, C.A.Bingman, S.Gruschow, D.H.Sherman, G.N.Phillips, J.S.Thorson. Structural Characterization of the Mitomycin 7-O-Methyltransferase. Proteins V. 79 2181 2011.
ISSN: ISSN 0887-3585
PubMed: 21538548
DOI: 10.1002/PROT.23040