Calcium in PDB 3kuc: Complex of RAP1A(E30D/K31E)Gdp with Rafrbd(A85K/N71R)

All present enzymatic activity of Complex of RAP1A(E30D/K31E)Gdp with Rafrbd(A85K/N71R):
2.7.11.1;

The structure of Complex of RAP1A(E30D/K31E)Gdp with Rafrbd(A85K/N71R), PDB code: 3kuc was solved by D.Filchtinski, O.Sharabi, A.Rueppel, I.R.Vetter, C.Herrmann, J.M.Shifman, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	36.50 / 1.92
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	43.120, 68.540, 101.630, 90.00, 90.00, 90.00
R / Rfree (%)	17.4 / 20.9

The structure of Complex of RAP1A(E30D/K31E)Gdp with Rafrbd(A85K/N71R) also contains other interesting chemical elements:

Magnesium

(Mg)

1 atom

The binding sites of Calcium atom in the Complex of RAP1A(E30D/K31E)Gdp with Rafrbd(A85K/N71R) (pdb code 3kuc). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total only one binding site of Calcium was determined in the Complex of RAP1A(E30D/K31E)Gdp with Rafrbd(A85K/N71R), PDB code: 3kuc:

Calcium binding site 1 out of 1 in the Complex of RAP1A(E30D/K31E)Gdp with Rafrbd(A85K/N71R)


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Complex of RAP1A(E30D/K31E)Gdp with Rafrbd(A85K/N71R) within 5.0Å range: probe atom residue distance (Å) B Occ B:Ca172 b:16.4 occ:1.00 O B:HOH249 2.3 13.6 1.0 O B:HOH242 2.3 19.9 1.0 O B:GLY123 2.4 23.2 1.0 OE2 B:GLU125 2.5 22.0 1.0 OE1 B:GLU125 2.5 16.8 1.0 CD B:GLU125 2.9 24.9 1.0 C B:GLY123 3.6 24.3 1.0 O B:HOH253 4.1 37.0 1.0 CD2 B:HIS103 4.2 15.4 1.0 NE2 B:HIS103 4.2 18.8 1.0 CA B:GLU124 4.3 17.2 1.0 CG B:GLU125 4.4 19.3 1.0 N B:GLU124 4.4 17.6 1.0 CA B:GLY123 4.5 23.8 1.0 O B:HOH205 4.6 16.3 1.0 N B:GLU125 4.6 15.4 1.0 O B:HOH237 4.6 25.1 1.0 C B:GLU124 4.6 19.2 1.0

D.Filchtinski, O.Sharabi, A.Ruppel, I.R.Vetter, C.Herrmann, J.M.Shifman. What Makes Ras An Efficient Molecular Switch: A Computational, Biophysical, and Structural Study of Ras-Gdp Interactions with Mutants of Raf. J.Mol.Biol. V. 399 422 2010.
ISSN: ISSN 0022-2836
PubMed: 20361980
DOI: 10.1016/J.JMB.2010.03.046