Calcium in PDB 3oxq: Crystal Structure of CA2+/Cam-CAV1.2 Pre-Iq/Iq Domain Complex

The structure of Crystal Structure of CA2+/Cam-CAV1.2 Pre-Iq/Iq Domain Complex, PDB code: 3oxq was solved by E.Y.Kim, C.H.Rumpf, F.Van Petegem, R.Arant, F.Findeisen, E.S.Cooley, E.Y.Isacoff, D.L.Minor, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	30.00 / 2.55
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	39.716, 132.050, 158.222, 90.00, 90.00, 90.00
R / Rfree (%)	24.1 / 28.8

Pages:

>>> Page 1 <<< Page 2, Binding sites: 11 - 14;

Binding sites:

The binding sites of Calcium atom in the Crystal Structure of CA2+/Cam-CAV1.2 Pre-Iq/Iq Domain Complex (pdb code 3oxq). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 14 binding sites of Calcium where determined in the Crystal Structure of CA2+/Cam-CAV1.2 Pre-Iq/Iq Domain Complex, PDB code: 3oxq:
Jump to Calcium binding site number: 1; 2; 3; 4; 5; 6; 7; 8; 9; 10;

Calcium binding site 1 out of 14 in the Crystal Structure of CA2+/Cam-CAV1.2 Pre-Iq/Iq Domain Complex


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Crystal Structure of CA2+/Cam-CAV1.2 Pre-Iq/Iq Domain Complex within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca501 b:45.3 occ:1.00 O A:HOH157 2.1 38.3 1.0 OD1 A:ASP20 2.2 40.5 1.0 O A:THR26 2.4 43.2 1.0 OD1 A:ASP22 2.4 44.4 1.0 OE2 A:GLU31 2.5 44.2 1.0 OD2 A:ASP24 2.6 48.0 1.0 OE1 A:GLU31 2.7 42.4 1.0 CD A:GLU31 2.9 43.2 1.0 CG A:ASP24 3.2 45.8 1.0 CG A:ASP22 3.2 44.5 1.0 CG A:ASP20 3.4 41.6 1.0 OD2 A:ASP22 3.5 46.4 1.0 OD1 A:ASP24 3.5 51.5 1.0 C A:THR26 3.6 39.5 1.0 OG1 A:THR26 3.9 45.5 1.0 OD2 A:ASP20 4.1 44.1 1.0 N A:THR26 4.2 39.9 1.0 N A:ASP24 4.2 42.1 1.0 CB A:ASP20 4.4 39.2 1.0 CB A:ASP24 4.4 41.9 1.0 CA A:THR26 4.4 40.7 1.0 N A:ASP22 4.4 42.2 1.0 CA A:ASP20 4.4 40.2 1.0 CG A:GLU31 4.4 43.2 1.0 N A:ILE27 4.5 32.6 1.0 CB A:ASP22 4.5 41.6 1.0 CA A:ILE27 4.6 31.0 1.0 N A:LYS21 4.6 40.4 1.0 C A:ASP20 4.6 41.4 1.0 N A:GLY23 4.7 40.1 1.0 CA A:ASP24 4.7 41.7 1.0 CB A:THR26 4.8 43.1 1.0 N A:GLY25 4.8 40.0 1.0 CA A:ASP22 4.8 42.5 1.0 N A:THR28 4.9 31.9 1.0 C A:ASP24 4.9 39.5 1.0 C A:ASP22 5.0 41.3 1.0

Calcium binding site 2 out of 14 in the Crystal Structure of CA2+/Cam-CAV1.2 Pre-Iq/Iq Domain Complex


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of Crystal Structure of CA2+/Cam-CAV1.2 Pre-Iq/Iq Domain Complex within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca502 b:39.3 occ:1.00 O A:HOH158 2.2 30.1 1.0 OD1 A:ASN60 2.4 44.4 1.0 OD1 A:ASP56 2.4 45.4 1.0 O A:THR62 2.4 41.0 1.0 OE1 A:GLU67 2.4 41.7 1.0 OE2 A:GLU67 2.4 42.6 1.0 O A:HOH152 2.5 37.1 1.0 OD2 A:ASP58 2.5 49.4 1.0 CD A:GLU67 2.8 38.7 1.0 CG A:ASN60 3.4 43.8 1.0 CG A:ASP58 3.4 46.2 1.0 CG A:ASP56 3.5 44.6 1.0 C A:THR62 3.6 39.4 1.0 OD1 A:ASP58 3.8 50.6 1.0 ND2 A:ASN60 4.0 44.0 1.0 OD2 A:ASP56 4.2 48.5 1.0 CG A:GLU67 4.3 33.3 1.0 N A:THR62 4.3 40.2 1.0 N A:ASN60 4.3 41.6 1.0 N A:ASP64 4.4 37.3 1.0 OD2 A:ASP64 4.4 47.2 1.0 OG1 A:THR62 4.4 40.4 1.0 CA A:ASP56 4.4 44.2 1.0 CB A:ASP56 4.4 42.9 1.0 N A:ASP58 4.4 35.0 1.0 CA A:ILE63 4.5 35.7 1.0 N A:ILE63 4.5 37.6 1.0 CB A:ASN60 4.6 44.4 1.0 CA A:THR62 4.6 40.0 1.0 CB A:ASP58 4.6 37.8 1.0 CG A:ASP64 4.7 45.1 1.0 C A:ASP56 4.7 43.9 1.0 N A:GLY61 4.8 42.9 1.0 CA A:ASN60 4.8 43.0 1.0 N A:ALA57 4.8 39.8 1.0 C A:ILE63 4.8 37.1 1.0 N A:GLY59 4.9 41.1 1.0 CA A:ASP58 4.9 37.2 1.0

Calcium binding site 3 out of 14 in the Crystal Structure of CA2+/Cam-CAV1.2 Pre-Iq/Iq Domain Complex


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 3 of Crystal Structure of CA2+/Cam-CAV1.2 Pre-Iq/Iq Domain Complex within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca503 b:54.7 occ:1.00 O A:TYR99 2.1 57.3 1.0 OD1 A:ASP95 2.2 73.1 1.0 OD2 A:ASP93 2.3 66.3 1.0 OE2 A:GLU104 2.4 54.9 1.0 OE1 A:GLU104 2.5 55.4 1.0 OD1 A:ASN97 2.7 64.4 1.0 CD A:GLU104 2.8 54.8 1.0 CG A:ASP95 3.1 73.9 1.0 CG A:ASP93 3.4 65.5 1.0 C A:TYR99 3.4 56.5 1.0 CG A:ASN97 3.5 65.7 1.0 OD2 A:ASP95 3.5 74.9 1.0 CA A:ASP93 4.1 61.5 1.0 N A:TYR99 4.1 56.6 1.0 OD1 A:ASP93 4.1 67.7 1.0 ND2 A:ASN97 4.2 64.5 1.0 CB A:ASP93 4.2 61.9 1.0 CA A:TYR99 4.2 58.0 1.0 N A:ASP95 4.2 71.5 1.0 CG A:GLU104 4.3 53.5 1.0 N A:ILE100 4.3 52.8 1.0 CA A:ILE100 4.3 50.2 1.0 C A:ASP93 4.3 63.8 1.0 N A:ASN97 4.4 65.3 1.0 CB A:ASP95 4.4 74.6 1.0 CB A:ASN97 4.5 67.3 1.0 N A:LYS94 4.5 65.3 1.0 N A:SER101 4.6 51.7 1.0 CB A:TYR99 4.7 58.7 1.0 CA A:ASP95 4.7 73.3 1.0 N A:GLY96 4.7 67.2 1.0 CA A:ASN97 4.8 65.4 1.0 C A:ASP95 4.9 72.2 1.0 N A:GLY98 4.9 60.5 1.0 O A:ASP93 4.9 63.2 1.0 C A:ILE100 5.0 51.1 1.0

Calcium binding site 4 out of 14 in the Crystal Structure of CA2+/Cam-CAV1.2 Pre-Iq/Iq Domain Complex


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 4 of Crystal Structure of CA2+/Cam-CAV1.2 Pre-Iq/Iq Domain Complex within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca504 b:66.7 occ:1.00 O A:GLN135 2.3 62.6 1.0 OD2 A:ASP129 2.4 74.4 1.0 OE2 A:GLU140 2.5 56.9 1.0 OE1 A:GLU140 2.6 53.9 1.0 OD2 A:ASP133 2.6 78.2 1.0 OD1 A:ASP131 2.8 84.2 1.0 CD A:GLU140 2.9 55.4 1.0 CG A:ASP133 3.2 79.6 1.0 CG A:ASP129 3.3 73.0 1.0 OD1 A:ASP133 3.3 82.1 1.0 CG A:ASP131 3.3 84.6 1.0 C A:GLN135 3.5 60.5 1.0 OD2 A:ASP131 3.5 85.5 1.0 OD1 A:ASP129 3.8 73.9 1.0 CB A:ASP129 4.2 72.8 1.0 N A:GLN135 4.2 65.3 1.0 CA A:ASP129 4.3 74.0 1.0 N A:ASP133 4.3 78.7 1.0 CB A:ASP131 4.4 85.8 1.0 N A:ASP131 4.4 84.2 1.0 N A:VAL136 4.4 56.0 1.0 CA A:GLN135 4.4 63.2 1.0 CA A:VAL136 4.4 55.8 1.0 CG A:GLU140 4.4 54.3 1.0 N A:ASN137 4.5 53.5 1.0 CB A:ASP133 4.5 80.2 1.0 N A:GLY132 4.6 82.4 1.0 CA A:ASP131 4.7 86.1 1.0 CA A:ASP133 4.8 77.8 1.0 C A:ASP129 4.8 77.5 1.0 N A:ILE130 4.8 79.9 1.0 CB A:GLN135 4.8 66.7 1.0 C A:ASP131 4.8 86.3 1.0 C A:VAL136 4.9 54.9 1.0 N A:GLY134 4.9 70.5 1.0 C A:ASP133 5.0 74.3 1.0

Calcium binding site 5 out of 14 in the Crystal Structure of CA2+/Cam-CAV1.2 Pre-Iq/Iq Domain Complex


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 5 of Crystal Structure of CA2+/Cam-CAV1.2 Pre-Iq/Iq Domain Complex within 5.0Å range: probe atom residue distance (Å) B Occ B:Ca505 b:58.6 occ:1.00 OE2 B:GLU31 2.0 62.7 1.0 O B:THR26 2.4 57.2 1.0 OD1 B:ASP20 2.5 58.8 1.0 OD1 B:ASP24 2.5 72.6 1.0 OD1 B:ASP22 2.5 74.7 1.0 CD B:GLU31 2.6 59.1 1.0 OE1 B:GLU31 2.6 58.8 1.0 CG B:ASP24 3.2 70.2 1.0 CG B:ASP22 3.4 74.3 1.0 OD2 B:ASP24 3.5 72.6 1.0 C B:THR26 3.6 52.2 1.0 CG B:ASP20 3.7 59.5 1.0 OD2 B:ASP22 3.7 74.4 1.0 OG1 B:THR26 4.1 57.3 1.0 CG B:GLU31 4.1 56.2 1.0 N B:ASP24 4.1 68.9 1.0 N B:THR26 4.2 55.9 1.0 CA B:ASP20 4.3 59.3 1.0 CB B:ASP24 4.3 70.2 1.0 CA B:THR26 4.4 55.8 1.0 N B:LYS21 4.5 64.6 1.0 OD2 B:ASP20 4.5 59.6 1.0 N B:ILE27 4.5 48.3 1.0 N B:ASP22 4.5 72.2 1.0 CA B:ILE27 4.6 45.6 1.0 CB B:ASP20 4.6 59.7 1.0 C B:ASP20 4.6 63.8 1.0 CA B:ASP24 4.7 67.3 1.0 N B:GLY23 4.7 72.8 1.0 CB B:ASP22 4.7 74.8 1.0 CG2 B:THR28 4.8 44.2 1.0 N B:GLY25 4.8 60.1 1.0 N B:THR28 4.9 47.6 1.0 CB B:THR26 4.9 56.9 1.0 CA B:ASP22 5.0 73.5 1.0 C B:ASP24 5.0 63.8 1.0

Calcium binding site 6 out of 14 in the Crystal Structure of CA2+/Cam-CAV1.2 Pre-Iq/Iq Domain Complex


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 6 of Crystal Structure of CA2+/Cam-CAV1.2 Pre-Iq/Iq Domain Complex within 5.0Å range: probe atom residue distance (Å) B Occ B:Ca506 b:41.8 occ:1.00 OD2 B:ASP56 2.1 48.5 1.0 OD1 B:ASN60 2.3 45.2 1.0 O B:THR62 2.3 46.5 1.0 O B:HOH150 2.5 35.7 1.0 OE1 B:GLU67 2.5 43.3 1.0 OE2 B:GLU67 2.5 43.8 1.0 OD1 B:ASP58 2.6 50.4 1.0 CD B:GLU67 2.8 42.4 1.0 CG B:ASP56 3.3 47.4 1.0 CG B:ASN60 3.3 47.4 1.0 CG B:ASP58 3.5 49.9 1.0 C B:THR62 3.5 45.4 1.0 OD2 B:ASP58 3.9 52.4 1.0 OD1 B:ASP56 4.0 48.7 1.0 ND2 B:ASN60 4.0 46.5 1.0 N B:ASN60 4.2 47.9 1.0 CA B:ILE63 4.3 42.0 1.0 N B:THR62 4.3 48.6 1.0 N B:ILE63 4.3 42.5 1.0 CG B:GLU67 4.4 36.6 1.0 N B:ASP58 4.4 45.1 1.0 CA B:ASP56 4.4 46.3 1.0 CB B:ASP56 4.4 45.3 1.0 CB B:ASN60 4.4 46.9 1.0 CA B:THR62 4.5 46.6 1.0 N B:ASP64 4.5 42.0 1.0 C B:ASP56 4.6 45.4 1.0 CB B:ASP58 4.6 46.7 1.0 OG1 B:THR62 4.7 47.0 1.0 N B:GLY61 4.7 48.1 1.0 N B:GLY59 4.7 47.5 1.0 CA B:ASN60 4.7 48.0 1.0 N B:ALA57 4.8 43.2 1.0 CA B:ASP58 4.8 47.3 1.0 C B:ILE63 4.9 42.4 1.0 C B:ASP58 4.9 49.2 1.0 OD2 B:ASP64 4.9 54.2 1.0 O B:ASP56 5.0 47.1 1.0 C B:ASN60 5.0 47.9 1.0

Calcium binding site 7 out of 14 in the Crystal Structure of CA2+/Cam-CAV1.2 Pre-Iq/Iq Domain Complex


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 7 of Crystal Structure of CA2+/Cam-CAV1.2 Pre-Iq/Iq Domain Complex within 5.0Å range: probe atom residue distance (Å) B Occ B:Ca508 b:0.3 occ:1.00 O B:GLN135 1.9 0.1 1.0 OE1 B:GLU140 2.2 0.2 1.0 OD1 B:ASP131 2.3 98.2 1.0 OD1 B:ASP133 2.6 0.9 1.0 OD1 B:ASP129 2.9 90.5 1.0 CG B:ASP131 3.0 98.6 1.0 C B:GLN135 3.1 0.5 1.0 OD2 B:ASP131 3.1 96.9 1.0 CD B:GLU140 3.2 0.1 1.0 OE2 B:GLU140 3.5 0.6 1.0 CG B:ASP133 3.5 0.4 1.0 N B:ASN137 3.7 0.5 1.0 CA B:VAL136 3.8 0.5 1.0 CG B:ASP129 3.8 89.9 1.0 OD2 B:ASP133 3.8 0.5 1.0 N B:VAL136 3.9 0.4 1.0 N B:GLN135 4.1 0.2 1.0 CA B:GLN135 4.2 0.5 1.0 C B:VAL136 4.2 0.5 1.0 CA B:ASP129 4.3 89.5 1.0 CB B:ASP131 4.4 99.1 1.0 CB B:ASP129 4.5 89.6 1.0 OD2 B:ASP129 4.5 90.8 1.0 CG B:GLU140 4.5 0.3 1.0 CB B:ASN137 4.6 0.0 1.0 N B:GLY134 4.6 0.9 1.0 N B:ASP133 4.7 0.5 1.0 CA B:ASN137 4.8 0.8 1.0 CB B:ASP133 4.8 0.6 1.0 N B:ASP131 4.9 97.4 1.0 C B:ASP129 4.9 89.9 1.0

Calcium binding site 8 out of 14 in the Crystal Structure of CA2+/Cam-CAV1.2 Pre-Iq/Iq Domain Complex


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 8 of Crystal Structure of CA2+/Cam-CAV1.2 Pre-Iq/Iq Domain Complex within 5.0Å range: probe atom residue distance (Å) B Occ C:Ca509 b:41.9 occ:1.00 O C:HOH153 2.1 42.7 1.0 OD1 C:ASP20 2.2 40.8 1.0 OE2 C:GLU31 2.3 44.7 1.0 O C:THR26 2.4 43.2 1.0 OD2 C:ASP24 2.5 47.9 1.0 OD1 C:ASP22 2.5 44.1 1.0 OE1 C:GLU31 2.8 42.7 1.0 CD C:GLU31 2.9 43.2 1.0 CG C:ASP22 3.2 44.4 1.0 CG C:ASP24 3.2 45.8 1.0 OD2 C:ASP22 3.3 46.3 1.0 CG C:ASP20 3.4 41.5 1.0 OD1 C:ASP24 3.5 51.6 1.0 C C:THR26 3.6 39.9 1.0 OG1 C:THR26 4.0 45.7 1.0 OD2 C:ASP20 4.1 44.6 1.0 N C:ASP24 4.2 42.3 1.0 N C:THR26 4.3 41.0 1.0 N C:ASP22 4.3 42.3 1.0 CB C:ASP24 4.4 41.8 1.0 CB C:ASP20 4.4 39.3 1.0 CG C:GLU31 4.4 43.0 1.0 CA C:THR26 4.4 41.0 1.0 CA C:ASP20 4.4 40.8 1.0 CB C:ASP22 4.4 41.9 1.0 N C:LYS21 4.6 40.7 1.0 N C:ILE27 4.6 33.5 1.0 C C:ASP20 4.6 42.1 1.0 CA C:ILE27 4.7 31.4 1.0 CA C:ASP24 4.7 41.6 1.0 N C:GLY23 4.7 39.8 1.0 CA C:ASP22 4.8 42.0 1.0 CB C:THR26 4.9 43.1 1.0 N C:GLY25 4.9 40.4 1.0 C C:ASP22 4.9 41.1 1.0 C C:ASP24 4.9 39.4 1.0 N C:THR28 4.9 32.1 1.0

Calcium binding site 9 out of 14 in the Crystal Structure of CA2+/Cam-CAV1.2 Pre-Iq/Iq Domain Complex


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 9 of Crystal Structure of CA2+/Cam-CAV1.2 Pre-Iq/Iq Domain Complex within 5.0Å range: probe atom residue distance (Å) B Occ C:Ca510 b:43.9 occ:1.00 O C:HOH151 2.1 27.1 1.0 OD1 C:ASP56 2.3 45.7 1.0 O C:THR62 2.3 40.9 1.0 OE1 C:GLU67 2.4 42.5 1.0 OD1 C:ASN60 2.4 44.1 1.0 OE2 C:GLU67 2.5 42.1 1.0 OD2 C:ASP58 2.6 49.6 1.0 O C:HOH152 2.7 36.4 1.0 CD C:GLU67 2.8 38.6 1.0 CG C:ASP56 3.3 45.4 1.0 CG C:ASN60 3.5 43.7 1.0 CG C:ASP58 3.5 46.3 1.0 C C:THR62 3.6 39.5 1.0 OD1 C:ASP58 3.9 50.9 1.0 OD2 C:ASP56 4.0 49.1 1.0 ND2 C:ASN60 4.1 44.2 1.0 N C:THR62 4.3 41.0 1.0 CA C:ASP56 4.3 44.3 1.0 CB C:ASP56 4.3 43.1 1.0 N C:ASN60 4.3 42.2 1.0 CG C:GLU67 4.3 32.8 1.0 N C:ASP64 4.3 37.1 1.0 CA C:ILE63 4.4 36.1 1.0 N C:ILE63 4.4 38.3 1.0 N C:ASP58 4.4 35.3 1.0 OG1 C:THR62 4.4 42.0 1.0 CA C:THR62 4.5 40.4 1.0 OD2 C:ASP64 4.6 46.7 1.0 C C:ASP56 4.6 44.0 1.0 N C:ALA57 4.6 39.8 1.0 CB C:ASN60 4.6 44.6 1.0 N C:GLY61 4.7 43.3 1.0 CB C:ASP58 4.7 37.3 1.0 CG C:ASP64 4.7 45.3 1.0 C C:ILE63 4.8 37.2 1.0 CA C:ASN60 4.8 44.0 1.0 N C:GLY59 4.8 40.8 1.0 CA C:ASP58 5.0 37.1 1.0

Calcium binding site 10 out of 14 in the Crystal Structure of CA2+/Cam-CAV1.2 Pre-Iq/Iq Domain Complex


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 10 of Crystal Structure of CA2+/Cam-CAV1.2 Pre-Iq/Iq Domain Complex within 5.0Å range: probe atom residue distance (Å) B Occ C:Ca511 b:60.9 occ:1.00 O C:TYR99 2.1 56.2 1.0 OD1 C:ASP95 2.3 73.2 1.0 OD2 C:ASP93 2.4 66.7 1.0 OE2 C:GLU104 2.5 54.1 1.0 OD1 C:ASN97 2.5 63.6 1.0 OE1 C:GLU104 2.6 54.6 1.0 CD C:GLU104 2.9 53.8 1.0 CG C:ASP95 3.2 73.8 1.0 C C:TYR99 3.3 55.4 1.0 CG C:ASP93 3.4 66.0 1.0 CG C:ASN97 3.4 64.9 1.0 OD2 C:ASP95 3.6 74.1 1.0 N C:TYR99 4.0 55.4 1.0 ND2 C:ASN97 4.1 63.3 1.0 OD1 C:ASP93 4.1 67.9 1.0 CA C:TYR99 4.2 56.3 1.0 CA C:ASP93 4.2 61.7 1.0 N C:ILE100 4.3 51.3 1.0 CB C:ASP93 4.3 62.0 1.0 N C:ASN97 4.3 65.5 1.0 N C:ASP95 4.3 71.9 1.0 CA C:ILE100 4.4 48.9 1.0 CG C:GLU104 4.4 52.0 1.0 CB C:ASP95 4.4 74.9 1.0 CB C:ASN97 4.4 67.0 1.0 C C:ASP93 4.5 64.2 1.0 N C:SER101 4.6 49.9 1.0 CB C:TYR99 4.6 57.0 1.0 N C:LYS94 4.6 65.2 1.0 CA C:ASP95 4.7 73.7 1.0 N C:GLY96 4.8 68.6 1.0 CA C:ASN97 4.8 65.5 1.0 N C:GLY98 4.8 60.8 1.0 C C:ASP95 4.9 73.1 1.0 C C:ILE100 5.0 49.1 1.0

E.Y.Kim, C.H.Rumpf, F.Van Petegem, R.J.Arant, F.Findeisen, E.S.Cooley, E.Y.Isacoff, D.L.Minor. Multiple C-Terminal Tail Ca(2+)/Cams Regulate Ca(V)1.2 Function But Do Not Mediate Channel Dimerization. Embo J. V. 29 3924 2010.
ISSN: ISSN 0261-4189
PubMed: 20953164
DOI: 10.1038/EMBOJ.2010.260