Calcium in PDB 3p2p: Enhanced Activity and Altered Specificity of Phospholipase A2 By Deletion of A Surface Loop

All present enzymatic activity of Enhanced Activity and Altered Specificity of Phospholipase A2 By Deletion of A Surface Loop:
3.1.1.4;

The structure of Enhanced Activity and Altered Specificity of Phospholipase A2 By Deletion of A Surface Loop, PDB code: 3p2p was solved by B.W.Dijkstra, M.M.G.M.Thunnissen, K.H.Kalk, J.Drenth, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	N/A / 2.10
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	45.800, 73.400, 37.200, 90.00, 107.40, 90.00
R / Rfree (%)	n/a / n/a

The binding sites of Calcium atom in the Enhanced Activity and Altered Specificity of Phospholipase A2 By Deletion of A Surface Loop (pdb code 3p2p). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 3 binding sites of Calcium where determined in the Enhanced Activity and Altered Specificity of Phospholipase A2 By Deletion of A Surface Loop, PDB code: 3p2p:
Jump to Calcium binding site number: 1; 2; 3;

Calcium binding site 1 out of 3 in the Enhanced Activity and Altered Specificity of Phospholipase A2 By Deletion of A Surface Loop


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Enhanced Activity and Altered Specificity of Phospholipase A2 By Deletion of A Surface Loop within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca125 b:26.9 occ:1.00 OD1 A:ASP49 2.4 19.9 1.0 O A:GLY30 2.4 31.9 1.0 OD2 A:ASP49 2.4 19.7 1.0 O A:TYR28 2.6 25.1 1.0 CG A:ASP49 2.7 19.9 1.0 O A:GLY32 2.9 36.9 1.0 C A:GLY32 3.5 36.3 1.0 C A:TYR28 3.6 25.5 1.0 C A:GLY30 3.6 32.7 1.0 N A:GLY30 3.8 31.0 1.0 CA A:GLY32 3.8 36.5 1.0 N A:GLY32 4.0 36.4 1.0 CA A:TYR28 4.1 25.4 1.0 O A:HOH395 4.2 17.4 1.0 CB A:ASP49 4.3 20.1 1.0 C A:LEU31 4.3 36.2 1.0 O A:HOH463 4.4 27.6 1.0 CA A:GLY30 4.4 31.9 1.0 O A:LEU31 4.4 36.6 1.0 N A:GLY33 4.4 35.2 1.0 O A:HOH491 4.4 46.5 1.0 O A:HOH448 4.5 23.7 1.0 CB A:TYR28 4.5 24.4 1.0 N A:CYS29 4.6 26.2 1.0 N A:LEU31 4.7 34.2 1.0 C A:CYS29 4.7 29.5 1.0 CA A:CYS29 4.8 26.9 1.0 CA A:GLY33 4.8 34.0 1.0 O A:CYS45 4.8 21.6 1.0

Calcium binding site 2 out of 3 in the Enhanced Activity and Altered Specificity of Phospholipase A2 By Deletion of A Surface Loop


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of Enhanced Activity and Altered Specificity of Phospholipase A2 By Deletion of A Surface Loop within 5.0Å range: probe atom residue distance (Å) B Occ B:Ca125 b:29.5 occ:1.00 O B:TYR28 2.3 31.7 1.0 O B:GLY32 2.4 36.3 1.0 OD1 B:ASP49 2.4 29.3 1.0 O B:GLY30 2.5 34.1 1.0 OD2 B:ASP49 2.5 28.9 1.0 CG B:ASP49 2.8 28.4 1.0 C B:TYR28 3.5 31.9 1.0 C B:GLY32 3.6 36.7 1.0 C B:GLY30 3.7 34.5 1.0 N B:GLY32 3.9 36.9 1.0 CA B:TYR28 4.2 32.4 1.0 N B:GLY30 4.2 32.8 1.0 CB B:ASP49 4.3 26.5 1.0 O B:HOH335 4.3 23.4 1.0 CA B:LEU31 4.4 36.1 1.0 CA B:GLY32 4.4 36.9 1.0 C B:LEU31 4.5 36.8 1.0 N B:CYS29 4.5 31.6 1.0 O B:HOH341 4.5 22.1 1.0 CB B:TYR28 4.5 33.0 1.0 C B:CYS29 4.5 32.3 1.0 N B:LEU31 4.6 35.4 1.0 N B:GLY33 4.6 37.1 1.0 CA B:GLY30 4.6 33.8 1.0 CA B:CYS29 4.7 31.0 1.0 O B:HOH384 4.7 41.8 1.0 CA B:GLY33 4.8 37.5 1.0 CD1 B:TYR28 4.9 33.8 1.0 O B:CYS45 4.9 24.8 1.0 O B:HOH434 5.0 33.9 1.0

Calcium binding site 3 out of 3 in the Enhanced Activity and Altered Specificity of Phospholipase A2 By Deletion of A Surface Loop


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 3 of Enhanced Activity and Altered Specificity of Phospholipase A2 By Deletion of A Surface Loop within 5.0Å range: probe atom residue distance (Å) B Occ B:Ca126 b:17.5 occ:1.00 OE1 A:GLU92 2.3 18.3 1.0 OE1 B:GLU92 2.3 15.8 1.0 OE1 A:GLU71 2.3 19.9 1.0 OE1 B:GLU71 2.4 22.0 1.0 O A:SER72 2.4 20.2 1.0 O B:SER72 2.6 19.5 1.0 CD B:GLU92 3.3 16.0 1.0 CD A:GLU92 3.3 18.6 1.0 CD B:GLU71 3.5 21.9 1.0 CD A:GLU71 3.5 20.1 1.0 C A:SER72 3.6 19.6 1.0 C B:SER72 3.6 19.3 1.0 N B:SER72 3.7 20.1 1.0 CG B:GLU92 3.7 17.0 1.0 CG A:GLU92 3.8 18.9 1.0 N A:SER72 3.8 19.7 1.0 CA B:SER72 4.0 19.1 1.0 CA A:SER72 4.2 18.9 1.0 CB A:SER72 4.2 18.2 1.0 O A:HOH329 4.2 17.4 1.0 OE2 B:GLU71 4.3 21.8 1.0 CG B:GLU71 4.4 21.6 1.0 OE2 A:GLU71 4.4 19.6 1.0 OE2 B:GLU92 4.4 15.3 1.0 CG A:GLU71 4.4 20.8 1.0 OE2 A:GLU92 4.5 18.3 1.0 C B:GLU71 4.5 21.0 1.0 CB B:SER72 4.6 18.1 1.0 N A:TYR73 4.7 19.2 1.0 N B:TYR73 4.7 19.5 1.0 C A:GLU71 4.7 20.7 1.0 CA B:TYR73 4.9 19.9 1.0 CA B:GLU71 4.9 21.4 1.0 CA A:GLU71 4.9 20.9 1.0 CA A:TYR73 5.0 18.4 1.0

O.P.Kuipers, M.M.Thunnissen, P.De Geus, B.W.Dijkstra, J.Drenth, H.M.Verheij, G.H.De Haas. Enhanced Activity and Altered Specificity of Phospholipase A2 By Deletion of A Surface Loop. Science V. 244 82 1989.
ISSN: ISSN 0036-8075
PubMed: 2704992