Calcium in PDB 3p75: Crystal Structure of Staphylococcal Nuclease Variant Delta+Phs V104D at Cryogenic Temperature

All present enzymatic activity of Crystal Structure of Staphylococcal Nuclease Variant Delta+Phs V104D at Cryogenic Temperature:
3.1.31.1;

The structure of Crystal Structure of Staphylococcal Nuclease Variant Delta+Phs V104D at Cryogenic Temperature, PDB code: 3p75 was solved by V.S.Khangulov, J.L.Schlessman, A.Heroux, E.B.Garcia-Moreno, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	30.94 / 1.90
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	31.000, 60.791, 36.838, 90.00, 93.45, 90.00
R / Rfree (%)	18.7 / 23.9

The binding sites of Calcium atom in the Crystal Structure of Staphylococcal Nuclease Variant Delta+Phs V104D at Cryogenic Temperature (pdb code 3p75). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 2 binding sites of Calcium where determined in the Crystal Structure of Staphylococcal Nuclease Variant Delta+Phs V104D at Cryogenic Temperature, PDB code: 3p75:
Jump to Calcium binding site number: 1; 2;

Calcium binding site 1 out of 2 in the Crystal Structure of Staphylococcal Nuclease Variant Delta+Phs V104D at Cryogenic Temperature


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Crystal Structure of Staphylococcal Nuclease Variant Delta+Phs V104D at Cryogenic Temperature within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca1 b:31.4 occ:1.00 O A:HOH191 2.2 16.0 1.0 O A:HOH177 2.2 33.1 1.0 OD1 A:ASP40 2.3 34.4 1.0 O A:HOH162 2.4 23.2 1.0 OD2 A:ASP21 2.4 28.8 1.0 O A:THR41 2.5 33.6 1.0 O A:HOH163 2.6 22.4 1.0 CG A:ASP21 3.3 28.2 1.0 CG A:ASP40 3.5 30.5 1.0 OD1 A:ASP21 3.6 26.5 1.0 C A:THR41 3.6 31.6 1.0 N A:THR41 3.8 28.8 1.0 OG1 A:THR41 3.8 26.7 1.0 O4P A:THP142 4.0 36.9 1.0 NH2 A:ARG35 4.1 24.2 1.0 CA A:CA143 4.1 23.5 0.5 OE2 A:GLU43 4.3 59.9 1.0 CA A:THR41 4.3 28.8 1.0 O A:HOH189 4.3 19.8 1.0 OD2 A:ASP40 4.3 34.3 1.0 C A:ASP40 4.3 28.2 1.0 CA A:ASP40 4.4 27.6 1.0 CB A:ASP40 4.5 27.4 1.0 C A:PRO42 4.6 36.0 1.0 OE1 A:GLU43 4.6 56.0 1.0 O5P A:THP142 4.6 38.0 1.0 N A:GLU43 4.6 37.7 1.0 O A:PRO42 4.6 36.2 1.0 OD2 A:ASP19 4.7 44.7 1.0 CB A:THR41 4.7 30.9 1.0 N A:PRO42 4.7 31.7 1.0 CD A:GLU43 4.7 55.5 1.0 CB A:ASP21 4.7 25.8 1.0 CZ A:ARG35 4.8 25.2 1.0 P2 A:THP142 4.8 36.8 1.0 O A:HOH188 4.9 16.7 1.0 CA A:PRO42 5.0 36.0 1.0

Calcium binding site 2 out of 2 in the Crystal Structure of Staphylococcal Nuclease Variant Delta+Phs V104D at Cryogenic Temperature


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of Crystal Structure of Staphylococcal Nuclease Variant Delta+Phs V104D at Cryogenic Temperature within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca143 b:23.5 occ:0.50 OD1 A:ASP21 2.2 26.5 1.0 O A:HOH166 2.3 25.2 1.0 O A:HOH177 2.3 33.1 1.0 O5P A:THP142 2.3 38.0 1.0 OG1 A:THR22 2.7 23.2 0.5 OD2 A:ASP19 2.8 44.7 1.0 OD1 A:ASP19 2.9 36.4 1.0 CG A:ASP19 3.2 37.7 1.0 CG A:ASP21 3.4 28.2 1.0 CB A:THR22 3.6 28.8 0.5 CB A:THR22 3.6 30.8 0.5 CG2 A:THR22 3.7 32.6 0.5 P2 A:THP142 3.7 36.8 1.0 O A:HOH163 3.8 22.4 1.0 O4P A:THP142 4.0 36.9 1.0 OD2 A:ASP21 4.0 28.8 1.0 CA A:CA1 4.1 31.4 1.0 N A:THR22 4.3 26.5 1.0 C A:ASP21 4.4 26.1 1.0 O A:HOH162 4.4 23.2 1.0 NH1 A:ARG87 4.4 33.2 1.0 NE A:ARG35 4.4 26.8 1.0 OG1 A:THR22 4.5 35.1 0.5 OE1 A:GLU43 4.5 56.0 1.0 CD A:ARG35 4.5 28.0 1.0 N A:ASP21 4.6 24.9 1.0 O6P A:THP142 4.6 41.4 1.0 CA A:THR22 4.6 28.1 0.5 CA A:THR22 4.6 29.7 0.5 CB A:ASP21 4.6 25.8 1.0 CG2 A:THR22 4.6 31.0 0.5 O A:ASP21 4.7 26.9 1.0 CB A:ASP19 4.7 35.2 1.0 CA A:ASP21 4.7 25.3 1.0 O5' A:THP142 4.8 33.7 1.0 O A:HOH150 5.0 27.7 1.0

V.S.Khangulov, J.L.Schlessman, E.B.Garcia-Moreno. Crystal Structure of Staphylococcal Nuclease Variant Delta+Phs V104D at Cryogenic Temperature To Be Published.