Calcium in PDB 3qee: The Structure and Function of An Arabinan-Specific Alpha-1,2- Arabinofuranosidase Identified From Screening the Activities of Bacterial GH43 Glycoside Hydrolases

The structure of The Structure and Function of An Arabinan-Specific Alpha-1,2- Arabinofuranosidase Identified From Screening the Activities of Bacterial GH43 Glycoside Hydrolases, PDB code: 3qee was solved by A.Cartmell, L.S.Mckee, M.Pena, J.Larsbrink, H.Brumer, R.J.Lewis, A.Viks-Nielsen, H.J.Gilbert, J.Marles-Wright, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	69.64 / 1.64
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	47.290, 139.270, 50.460, 90.00, 115.95, 90.00
R / Rfree (%)	15.3 / 18.4

The binding sites of Calcium atom in the The Structure and Function of An Arabinan-Specific Alpha-1,2- Arabinofuranosidase Identified From Screening the Activities of Bacterial GH43 Glycoside Hydrolases (pdb code 3qee). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 2 binding sites of Calcium where determined in the The Structure and Function of An Arabinan-Specific Alpha-1,2- Arabinofuranosidase Identified From Screening the Activities of Bacterial GH43 Glycoside Hydrolases, PDB code: 3qee:
Jump to Calcium binding site number: 1; 2;

Calcium binding site 1 out of 2 in the The Structure and Function of An Arabinan-Specific Alpha-1,2- Arabinofuranosidase Identified From Screening the Activities of Bacterial GH43 Glycoside Hydrolases


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of The Structure and Function of An Arabinan-Specific Alpha-1,2- Arabinofuranosidase Identified From Screening the Activities of Bacterial GH43 Glycoside Hydrolases within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca21 b:12.6 occ:1.00 OD2 A:ASP166 2.4 10.1 1.0 OG1 A:THR157 2.4 15.2 1.0 OD1 A:ASN154 2.4 12.7 1.0 O A:THR157 2.5 16.1 1.0 OD2 A:ASP165 2.5 13.1 1.0 OD1 A:ASP165 2.5 13.8 1.0 O A:ASN154 2.5 13.1 1.0 O A:HOH502 2.6 15.4 1.0 CG A:ASP165 2.8 11.9 1.0 CG A:ASP166 3.4 9.2 1.0 C A:ASN154 3.5 13.4 1.0 C A:THR157 3.5 17.0 1.0 CB A:THR157 3.6 15.8 1.0 CG A:ASN154 3.6 12.3 1.0 CA A:ASN154 3.7 12.4 1.0 OD1 A:ASP166 3.8 8.7 1.0 CA A:THR157 4.0 16.2 1.0 N A:THR157 4.2 15.6 1.0 O A:HOH408 4.2 11.1 1.0 CB A:ASN154 4.2 12.4 1.0 O A:HOH394 4.3 21.3 1.0 CB A:ASP165 4.4 11.5 1.0 O A:ILE162 4.5 19.7 1.0 ND2 A:ASN154 4.6 11.8 1.0 NH1 A:ARG189 4.6 15.7 1.0 CB A:ASP166 4.7 8.7 1.0 N A:THR158 4.7 18.8 1.0 N A:ASP155 4.7 14.3 1.0 NH2 A:ARG189 4.8 14.9 1.0 N A:ASP166 4.8 9.7 1.0 CG2 A:THR157 4.8 16.0 1.0 O A:THR153 5.0 10.3 1.0 O A:THR160 5.0 23.4 1.0 N A:ASN154 5.0 11.7 1.0

Calcium binding site 2 out of 2 in the The Structure and Function of An Arabinan-Specific Alpha-1,2- Arabinofuranosidase Identified From Screening the Activities of Bacterial GH43 Glycoside Hydrolases


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of The Structure and Function of An Arabinan-Specific Alpha-1,2- Arabinofuranosidase Identified From Screening the Activities of Bacterial GH43 Glycoside Hydrolases within 5.0Å range: probe atom residue distance (Å) B Occ B:Ca22 b:14.0 occ:1.00 O B:THR157 2.4 17.3 1.0 OD2 B:ASP166 2.4 13.2 1.0 OD1 B:ASN154 2.4 12.5 1.0 O B:HOH428 2.5 15.3 1.0 OG1 B:THR157 2.5 15.1 1.0 OD2 B:ASP165 2.5 14.0 1.0 OD1 B:ASP165 2.5 12.6 1.0 O B:ASN154 2.6 13.3 1.0 CG B:ASP165 2.8 13.1 1.0 C B:THR157 3.5 17.5 1.0 CG B:ASP166 3.5 11.1 1.0 C B:ASN154 3.5 13.8 1.0 CG B:ASN154 3.6 13.5 1.0 CB B:THR157 3.6 15.9 1.0 CA B:ASN154 3.7 13.2 1.0 OD1 B:ASP166 3.9 9.8 1.0 CA B:THR157 3.9 16.4 1.0 N B:THR157 4.2 16.1 1.0 O B:HOH478 4.2 12.8 1.0 CB B:ASN154 4.2 13.1 1.0 O B:HOH512 4.3 16.2 1.0 CB B:ASP165 4.4 12.7 1.0 O B:ILE162 4.5 18.8 1.0 N B:THR158 4.6 19.2 1.0 ND2 B:ASN154 4.6 13.1 1.0 CB B:ASP166 4.7 9.9 1.0 N B:ASP155 4.7 14.9 1.0 NH1 B:ARG189 4.7 16.6 1.0 NH2 B:ARG189 4.8 16.6 1.0 N B:ASP166 4.8 10.0 1.0 O B:THR160 4.8 22.5 1.0 CG2 B:THR157 4.9 14.6 1.0 N B:ASN154 5.0 12.4 1.0 O B:THR153 5.0 11.0 1.0

A.Cartmell, L.S.Mckee, M.J.Pena, J.Larsbrink, H.Brumer, S.Kaneko, H.Ichinose, R.J.Lewis, A.Vikso-Nielsen, H.J.Gilbert, J.Marles-Wright. The Structure and Function of An Arabinan-Specific {Alpha}-1,2-Arabinofuranosidase Identified From Screening the Activities of Bacterial GH43 Glycoside Hydrolases. J.Biol.Chem. V. 286 15483 2011.
ISSN: ISSN 0021-9258
PubMed: 21339299
DOI: 10.1074/JBC.M110.215962