Calcium in PDB 3qef: The Structure and Function of An Arabinan-Specific Alpha-1,2- Arabinofuranosidase Identified From Screening the Activities of Bacterial GH43 Glycoside Hydrolases

The structure of The Structure and Function of An Arabinan-Specific Alpha-1,2- Arabinofuranosidase Identified From Screening the Activities of Bacterial GH43 Glycoside Hydrolases, PDB code: 3qef was solved by A.Cartmell, L.S.Mckee, M.Pena, J.Larsbrink, H.Brumer, R.J.Lewis, A.Viks-Nielsen, H.J.Gilbert, J.Marles-Wright, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	44.37 / 1.79
Space group	P 41 21 2
Cell size a, b, c (Å), α, β, γ (°)	85.640, 85.640, 195.560, 90.00, 90.00, 90.00
R / Rfree (%)	16.9 / 19.8

The binding sites of Calcium atom in the The Structure and Function of An Arabinan-Specific Alpha-1,2- Arabinofuranosidase Identified From Screening the Activities of Bacterial GH43 Glycoside Hydrolases (pdb code 3qef). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 2 binding sites of Calcium where determined in the The Structure and Function of An Arabinan-Specific Alpha-1,2- Arabinofuranosidase Identified From Screening the Activities of Bacterial GH43 Glycoside Hydrolases, PDB code: 3qef:
Jump to Calcium binding site number: 1; 2;

Calcium binding site 1 out of 2 in the The Structure and Function of An Arabinan-Specific Alpha-1,2- Arabinofuranosidase Identified From Screening the Activities of Bacterial GH43 Glycoside Hydrolases


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of The Structure and Function of An Arabinan-Specific Alpha-1,2- Arabinofuranosidase Identified From Screening the Activities of Bacterial GH43 Glycoside Hydrolases within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca335 b:23.0 occ:1.00 O A:THR157 2.4 26.3 1.0 OD1 A:ASN154 2.4 25.4 1.0 OD2 A:ASP166 2.4 22.8 1.0 OD2 A:ASP165 2.5 22.1 1.0 OG1 A:THR157 2.5 23.1 1.0 O A:HOH363 2.6 22.7 1.0 O A:ASN154 2.6 24.4 1.0 OD1 A:ASP165 2.6 21.1 1.0 CG A:ASP165 2.9 21.1 1.0 CG A:ASP166 3.5 22.6 1.0 C A:THR157 3.5 25.4 1.0 C A:ASN154 3.5 24.7 1.0 CG A:ASN154 3.6 24.3 1.0 CB A:THR157 3.6 22.6 1.0 CA A:ASN154 3.7 24.7 1.0 OD1 A:ASP166 3.8 22.2 1.0 CA A:THR157 3.9 23.8 1.0 N A:THR157 4.1 25.8 1.0 O A:HOH499 4.2 32.0 1.0 CB A:ASN154 4.2 24.0 1.0 O A:HOH349 4.2 22.2 1.0 CB A:ASP165 4.4 18.7 1.0 O A:ILE162 4.5 26.5 1.0 ND2 A:ASN154 4.6 25.5 1.0 N A:THR158 4.6 24.9 1.0 N A:ASP155 4.7 25.6 1.0 CB A:ASP166 4.7 17.7 1.0 NH1 A:ARG189 4.8 23.4 1.0 NH2 A:ARG189 4.8 21.4 1.0 N A:ASP166 4.8 17.7 1.0 CG2 A:THR157 4.9 23.2 1.0 CA A:THR158 5.0 26.7 1.0 O A:THR153 5.0 23.7 1.0

Calcium binding site 2 out of 2 in the The Structure and Function of An Arabinan-Specific Alpha-1,2- Arabinofuranosidase Identified From Screening the Activities of Bacterial GH43 Glycoside Hydrolases


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of The Structure and Function of An Arabinan-Specific Alpha-1,2- Arabinofuranosidase Identified From Screening the Activities of Bacterial GH43 Glycoside Hydrolases within 5.0Å range: probe atom residue distance (Å) B Occ B:Ca335 b:26.8 occ:1.00 OD1 B:ASN154 2.4 27.8 1.0 O B:THR157 2.5 31.6 1.0 OD2 B:ASP166 2.5 25.4 1.0 O B:ASN154 2.5 26.9 1.0 OG1 B:THR157 2.5 27.8 1.0 O B:HOH457 2.6 29.3 1.0 OD2 B:ASP165 2.6 25.6 1.0 OD1 B:ASP165 2.6 27.9 1.0 CG B:ASP165 3.0 26.2 1.0 C B:ASN154 3.4 29.5 1.0 CG B:ASP166 3.5 24.2 1.0 C B:THR157 3.5 36.3 1.0 CG B:ASN154 3.5 31.2 1.0 CB B:THR157 3.6 27.2 1.0 CA B:ASN154 3.7 25.9 1.0 OD1 B:ASP166 3.8 25.1 1.0 CA B:THR157 3.9 32.5 1.0 N B:THR157 4.1 29.6 1.0 CB B:ASN154 4.2 26.7 1.0 O B:HOH368 4.2 22.8 1.0 O B:HOH544 4.3 36.0 1.0 CB B:ASP165 4.5 23.4 1.0 O B:ILE162 4.5 30.7 1.0 ND2 B:ASN154 4.6 26.5 1.0 N B:ASP155 4.7 32.1 1.0 N B:THR158 4.7 37.2 1.0 NH1 B:ARG189 4.8 26.8 1.0 CB B:ASP166 4.8 24.8 1.0 N B:ASP166 4.8 22.1 1.0 CG2 B:THR157 4.9 28.3 1.0 NH2 B:ARG189 4.9 27.6 1.0 O B:THR160 5.0 30.3 1.0 O B:THR153 5.0 27.5 1.0 N B:ASN154 5.0 25.5 1.0

A.Cartmell, L.S.Mckee, M.J.Pena, J.Larsbrink, H.Brumer, S.Kaneko, H.Ichinose, R.J.Lewis, A.Vikso-Nielsen, H.J.Gilbert, J.Marles-Wright. The Structure and Function of An Arabinan-Specific {Alpha}-1,2-Arabinofuranosidase Identified From Screening the Activities of Bacterial GH43 Glycoside Hydrolases. J.Biol.Chem. V. 286 15483 2011.
ISSN: ISSN 0021-9258
PubMed: 21339299
DOI: 10.1074/JBC.M110.215962