Calcium in PDB 3rbx: Mthk Rck Domain D184N Mutant, CA2+-Bound

The structure of Mthk Rck Domain D184N Mutant, CA2+-Bound, PDB code: 3rbx was solved by E.Samakai, B.S.Rothberg, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	49.49 / 2.80
Space group	P 65 2 2
Cell size a, b, c (Å), α, β, γ (°)	119.120, 119.120, 351.052, 90.00, 90.00, 120.00
R / Rfree (%)	24 / 27.4

Pages:

>>> Page 1 <<< Page 2, Binding sites: 11 - 12;

Binding sites:

The binding sites of Calcium atom in the Mthk Rck Domain D184N Mutant, CA2+-Bound (pdb code 3rbx). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 12 binding sites of Calcium where determined in the Mthk Rck Domain D184N Mutant, CA2+-Bound, PDB code: 3rbx:
Jump to Calcium binding site number: 1; 2; 3; 4; 5; 6; 7; 8; 9; 10;

Calcium binding site 1 out of 12 in the Mthk Rck Domain D184N Mutant, CA2+-Bound


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Mthk Rck Domain D184N Mutant, CA2+-Bound within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca600 b:59.5 occ:1.00 OE1 A:GLU248 2.0 43.1 1.0 OE2 B:GLU266 2.1 53.6 1.0 O A:ARG241 2.5 44.3 1.0 CD B:GLU266 2.6 56.7 1.0 O A:ASP245 2.6 56.0 1.0 CD A:GLU248 2.7 60.0 1.0 OE1 B:GLU266 2.7 73.6 1.0 OE2 A:GLU248 2.7 65.0 1.0 C A:ARG241 3.7 30.8 1.0 C A:ASP245 3.7 50.2 1.0 CG B:GLU266 3.8 31.0 1.0 CG A:GLU248 4.2 46.5 1.0 N A:ASP245 4.2 75.6 1.0 CA A:ASP245 4.3 56.8 1.0 CB A:ASP245 4.3 63.0 1.0 CB A:ARG241 4.4 27.5 1.0 CA A:ARG241 4.4 32.9 1.0 N A:TYR247 4.7 35.3 1.0 N A:SER242 4.7 26.6 1.0 N A:GLU248 4.8 31.8 1.0 N A:GLY246 4.8 33.9 1.0 CB B:GLU266 4.8 28.8 1.0 CA A:SER242 4.8 25.3 1.0 CB A:GLU248 4.9 36.2 1.0

Calcium binding site 2 out of 12 in the Mthk Rck Domain D184N Mutant, CA2+-Bound


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of Mthk Rck Domain D184N Mutant, CA2+-Bound within 5.0Å range: probe atom residue distance (Å) B Occ B:Ca601 b:43.4 occ:1.00 OD2 A:ASP305 2.6 47.7 1.0 O B:GLY290 2.6 35.1 1.0 OE2 B:GLU326 2.7 29.6 1.0 OE1 B:GLU326 2.8 35.9 1.0 OD1 A:ASP305 2.8 47.3 1.0 CG A:ASP305 3.0 59.2 1.0 CD B:GLU326 3.1 41.2 1.0 C B:GLY290 3.7 36.7 1.0 O A:ASP305 4.0 47.7 1.0 CA B:GLY290 4.1 33.2 1.0 NH1 B:ARG262 4.1 59.6 1.0 O A:PRO306 4.4 68.6 1.0 CB A:ASP305 4.4 51.0 1.0 CE1 B:HIS286 4.5 33.8 1.0 CG B:GLU326 4.6 41.2 1.0 C A:ASP305 4.7 51.9 1.0 N B:VAL291 4.9 46.0 1.0

Calcium binding site 3 out of 12 in the Mthk Rck Domain D184N Mutant, CA2+-Bound


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 3 of Mthk Rck Domain D184N Mutant, CA2+-Bound within 5.0Å range: probe atom residue distance (Å) B Occ B:Ca600 b:76.7 occ:1.00 O B:ARG241 2.3 38.1 1.0 OE1 B:GLU248 2.7 40.2 1.0 OE2 B:GLU248 2.9 64.1 1.0 OE2 A:GLU266 3.1 57.2 1.0 O B:ASP245 3.1 73.3 1.0 OE1 A:GLU266 3.1 75.4 1.0 CD B:GLU248 3.2 58.8 1.0 CD A:GLU266 3.3 60.2 1.0 C B:ARG241 3.5 27.9 1.0 CA B:ARG241 4.1 27.5 1.0 C B:ASP245 4.2 69.0 1.0 N B:ASP245 4.2 83.7 1.0 CB B:ARG241 4.3 20.3 1.0 CG A:GLU266 4.5 32.1 1.0 N B:SER242 4.5 28.8 1.0 CA B:ASP245 4.7 67.8 1.0 CB B:ASP245 4.7 75.8 1.0 CG B:GLU248 4.8 37.0 1.0 CA B:SER242 4.8 28.3 1.0 CB B:ASP244 5.0 70.9 1.0

Calcium binding site 4 out of 12 in the Mthk Rck Domain D184N Mutant, CA2+-Bound


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 4 of Mthk Rck Domain D184N Mutant, CA2+-Bound within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca601 b:31.2 occ:1.00 O A:GLY290 2.4 47.5 1.0 OE1 A:GLU326 2.5 48.2 1.0 OE2 A:GLU326 2.5 39.8 1.0 OD2 B:ASP305 2.7 25.0 1.0 CD A:GLU326 2.8 49.8 1.0 OD1 B:ASP305 3.0 33.6 1.0 CG B:ASP305 3.2 39.5 1.0 C A:GLY290 3.4 46.0 1.0 CA A:GLY290 3.9 43.5 1.0 NH1 A:ARG262 4.2 52.0 1.0 O B:ASP305 4.3 31.6 1.0 CG A:GLU326 4.3 45.3 1.0 NZ A:LYS323 4.4 73.0 1.0 CE1 A:HIS286 4.5 53.1 1.0 N A:VAL291 4.6 37.1 1.0 CB B:ASP305 4.7 36.2 1.0 O B:PRO306 4.7 42.3 1.0 CA A:GLY322 5.0 24.9 1.0 CA A:VAL291 5.0 33.9 1.0

Calcium binding site 5 out of 12 in the Mthk Rck Domain D184N Mutant, CA2+-Bound


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 5 of Mthk Rck Domain D184N Mutant, CA2+-Bound within 5.0Å range: probe atom residue distance (Å) B Occ C:Ca600 b:69.7 occ:1.00 O C:ARG241 2.4 55.9 1.0 OE2 D:GLU266 2.9 61.3 1.0 OE1 D:GLU266 2.9 84.1 1.0 O C:ASP245 2.9 56.9 1.0 OE1 C:GLU248 2.9 49.8 1.0 CD D:GLU266 3.1 65.3 1.0 OE2 C:GLU248 3.2 66.7 1.0 CD C:GLU248 3.5 60.5 1.0 C C:ARG241 3.5 50.7 1.0 N C:ASP245 3.9 77.0 1.0 C C:ASP245 4.0 58.3 1.0 CA C:ARG241 4.2 48.6 1.0 CA C:ASP245 4.3 61.5 1.0 CG D:GLU266 4.4 40.7 1.0 CB C:ASP245 4.5 64.3 1.0 CB C:ARG241 4.5 41.4 1.0 N C:SER242 4.5 38.5 1.0 CB C:ASP244 4.7 96.3 1.0 CA C:SER242 4.7 32.8 1.0 N C:ASP244 4.8 79.3 1.0 C C:ASP244 5.0 92.8 1.0 CG C:GLU248 5.0 45.5 1.0

Calcium binding site 6 out of 12 in the Mthk Rck Domain D184N Mutant, CA2+-Bound


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 6 of Mthk Rck Domain D184N Mutant, CA2+-Bound within 5.0Å range: probe atom residue distance (Å) B Occ C:Ca601 b:75.2 occ:1.00 O D:GLY290 2.7 48.7 1.0 OD1 C:ASP305 2.9 75.4 1.0 OE2 D:GLU326 3.0 62.9 1.0 OD2 C:ASP305 3.2 70.3 1.0 CG C:ASP305 3.3 79.0 1.0 OE1 D:GLU326 3.4 59.9 1.0 CD D:GLU326 3.6 66.4 1.0 C D:GLY290 3.7 48.6 1.0 O C:PRO306 3.9 89.3 1.0 CA D:GLY290 4.0 45.0 1.0 CE1 D:HIS286 4.0 67.2 1.0 O C:ASP305 4.0 69.7 1.0 NH1 D:ARG262 4.6 78.0 1.0 C C:ASP305 4.7 76.2 1.0 ND1 D:HIS286 4.7 75.7 1.0 CB C:ASP305 4.7 67.9 1.0 C C:PRO306 4.8 85.1 1.0 N D:VAL291 4.9 50.2 1.0 NE2 D:HIS286 4.9 70.8 1.0 CA C:PRO307 4.9 0.6 1.0

Calcium binding site 7 out of 12 in the Mthk Rck Domain D184N Mutant, CA2+-Bound


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 7 of Mthk Rck Domain D184N Mutant, CA2+-Bound within 5.0Å range: probe atom residue distance (Å) B Occ C:Ca341 b:0.7 occ:1.00 O D:ARG241 2.8 63.3 1.0 OE1 C:GLU266 2.9 87.0 1.0 O D:ASP245 3.1 73.2 1.0 OE2 C:GLU266 3.4 65.7 1.0 CD C:GLU266 3.4 65.8 1.0 N D:ASP245 3.5 86.0 1.0 OE1 D:GLU248 3.6 61.3 1.0 OE2 D:GLU248 3.8 70.6 1.0 C D:ARG241 3.9 53.8 1.0 C D:ASP245 4.0 72.4 1.0 CB D:ASP244 4.1 71.4 1.0 CD D:GLU248 4.1 69.3 1.0 CA D:ASP245 4.1 71.8 1.0 CB D:ASP245 4.2 68.6 1.0 N D:ASP244 4.4 51.4 1.0 C D:ASP244 4.5 58.2 1.0 CA D:ASP244 4.6 56.3 1.0 CA D:ARG241 4.7 52.3 1.0 CG C:GLU266 4.7 44.4 1.0 OD2 D:ASP244 4.9 87.3 1.0 N D:SER242 4.9 38.5 1.0 CG D:ASP244 5.0 76.7 1.0 OD1 D:ASP245 5.0 0.2 1.0 CA D:SER242 5.0 37.9 1.0

Calcium binding site 8 out of 12 in the Mthk Rck Domain D184N Mutant, CA2+-Bound


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 8 of Mthk Rck Domain D184N Mutant, CA2+-Bound within 5.0Å range: probe atom residue distance (Å) B Occ C:Ca342 b:65.7 occ:1.00 OD2 D:ASP305 2.5 47.2 1.0 OE1 C:GLU326 2.6 72.5 1.0 O C:GLY290 2.7 63.8 1.0 OE2 C:GLU326 2.8 85.3 1.0 OD1 D:ASP305 2.9 58.4 1.0 CG D:ASP305 3.0 61.8 1.0 CD C:GLU326 3.1 83.0 1.0 C C:GLY290 3.8 65.4 1.0 NH1 C:ARG262 3.9 62.7 1.0 O D:ASP305 4.0 52.6 1.0 NZ C:LYS323 4.2 91.2 1.0 CA C:GLY290 4.3 63.0 1.0 CB D:ASP305 4.4 57.6 1.0 CG C:GLU326 4.6 75.6 1.0 O D:PRO306 4.6 64.1 1.0 CE1 C:HIS286 4.6 89.6 1.0 C D:ASP305 4.8 63.6 1.0 N C:VAL291 4.9 58.1 1.0 CA C:GLY322 4.9 48.7 1.0 N C:LYS323 5.0 75.8 1.0

Calcium binding site 9 out of 12 in the Mthk Rck Domain D184N Mutant, CA2+-Bound


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 9 of Mthk Rck Domain D184N Mutant, CA2+-Bound within 5.0Å range: probe atom residue distance (Å) B Occ E:Ca600 b:71.8 occ:1.00 O E:ARG241 2.7 49.0 1.0 OE1 F:GLU266 2.9 90.2 1.0 OE2 F:GLU266 3.2 74.9 1.0 CD F:GLU266 3.3 73.5 1.0 O E:ASP245 3.3 64.0 1.0 OE1 E:GLU248 3.3 62.2 1.0 OE2 E:GLU248 3.4 73.6 1.0 CD E:GLU248 3.8 71.9 1.0 C E:ARG241 3.8 43.1 1.0 N E:ASP245 4.0 79.0 1.0 C E:ASP245 4.3 62.0 1.0 CA E:ARG241 4.4 40.4 1.0 CB E:ASP244 4.5 90.2 1.0 CG F:GLU266 4.5 49.8 1.0 CA E:ASP245 4.5 68.9 1.0 CB E:ASP245 4.6 67.7 1.0 CB E:ARG241 4.7 30.8 1.0 N E:ASP244 4.7 61.9 1.0 N E:SER242 4.9 42.5 1.0 CA E:ASP244 5.0 75.3 1.0

Calcium binding site 10 out of 12 in the Mthk Rck Domain D184N Mutant, CA2+-Bound


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 10 of Mthk Rck Domain D184N Mutant, CA2+-Bound within 5.0Å range: probe atom residue distance (Å) B Occ F:Ca601 b:56.0 occ:1.00 O F:GLY290 2.5 47.6 1.0 OD2 E:ASP305 2.7 42.2 1.0 OE2 F:GLU326 2.8 56.9 1.0 OE1 F:GLU326 2.8 56.6 1.0 OD1 E:ASP305 3.0 45.1 1.0 CD F:GLU326 3.2 64.8 1.0 CG E:ASP305 3.2 51.9 1.0 C F:GLY290 3.6 51.3 1.0 O E:ASP305 3.9 49.7 1.0 CA F:GLY290 4.1 46.0 1.0 NH1 F:ARG262 4.2 63.6 1.0 CE1 F:HIS286 4.3 59.9 1.0 O E:PRO306 4.5 63.0 1.0 CB E:ASP305 4.6 47.5 1.0 CG F:GLU326 4.7 60.5 1.0 N F:VAL291 4.8 48.6 1.0 C E:ASP305 4.8 51.0 1.0 ND1 F:HIS286 5.0 67.4 1.0

V.P.Pau, F.J.Smith, A.B.Taylor, E.Samakai, M.M.Callaghan, K.Abarca-Heidemann, L.V.Parfenova, P.J.Hart, B.S.Rothberg. Structures of Multiple CA2+-Binding Sites in A K+ Channel Rck Domain Proc.Natl.Acad.Sci.Usa 2011.
ISSN: ESSN 1091-6490