Calcium in PDB 3sjq: Crystal Structure of A Small Conductance Potassium Channel Splice Variant Complexed with Calcium-Calmodulin

The structure of Crystal Structure of A Small Conductance Potassium Channel Splice Variant Complexed with Calcium-Calmodulin, PDB code: 3sjq was solved by M.Zhang, J.M.Pascal, J.-F.Zhang, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	19.99 / 1.90
Space group	P 1
Cell size a, b, c (Å), α, β, γ (°)	47.740, 65.630, 66.080, 90.68, 110.45, 111.02
R / Rfree (%)	17 / 20.1

The binding sites of Calcium atom in the Crystal Structure of A Small Conductance Potassium Channel Splice Variant Complexed with Calcium-Calmodulin (pdb code 3sjq). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 8 binding sites of Calcium where determined in the Crystal Structure of A Small Conductance Potassium Channel Splice Variant Complexed with Calcium-Calmodulin, PDB code: 3sjq:
Jump to Calcium binding site number: 1; 2; 3; 4; 5; 6; 7; 8;

Calcium binding site 1 out of 8 in the Crystal Structure of A Small Conductance Potassium Channel Splice Variant Complexed with Calcium-Calmodulin


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Crystal Structure of A Small Conductance Potassium Channel Splice Variant Complexed with Calcium-Calmodulin within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca1001 b:22.0 occ:1.00 OD1 A:ASP20 2.2 25.2 1.0 O A:HOH155 2.3 21.7 1.0 O A:THR26 2.3 31.5 1.0 OD1 A:ASP24 2.4 21.4 1.0 OD1 A:ASP22 2.4 26.0 1.0 OE1 A:GLU31 2.5 22.1 1.0 OE2 A:GLU31 2.5 24.2 1.0 CD A:GLU31 2.8 24.5 1.0 CG A:ASP24 3.3 27.4 1.0 CG A:ASP22 3.3 36.2 1.0 CG A:ASP20 3.3 21.1 1.0 C A:THR26 3.5 26.1 1.0 OD2 A:ASP22 3.7 29.0 1.0 OD2 A:ASP24 3.9 32.4 1.0 OG1 A:THR26 3.9 35.6 1.0 N A:ASP24 4.1 24.1 1.0 OD2 A:ASP20 4.2 25.7 1.0 N A:THR26 4.2 21.5 1.0 N A:ASP22 4.2 22.5 1.0 CA A:ASP20 4.2 26.8 1.0 CB A:ASP20 4.3 28.9 1.0 CB A:ASP24 4.3 25.9 1.0 CG A:GLU31 4.3 24.5 1.0 CA A:THR26 4.4 18.2 1.0 C A:ASP20 4.4 27.2 1.0 N A:ILE27 4.5 23.4 1.0 CB A:ASP22 4.5 30.6 1.0 CA A:ILE27 4.5 24.2 1.0 CA A:ASP24 4.6 24.1 1.0 N A:GLY23 4.6 26.9 1.0 N A:LYS21 4.6 29.0 1.0 O A:HOH230 4.7 30.3 1.0 CA A:ASP22 4.7 25.6 1.0 N A:GLY25 4.7 22.4 1.0 CG2 A:THR28 4.7 20.7 1.0 CB A:THR26 4.8 29.7 1.0 C A:ASP22 4.8 23.0 1.0 N A:THR28 4.9 23.9 1.0 C A:ASP24 4.9 21.9 1.0 O A:ASP20 4.9 31.3 1.0 O A:HOH162 5.0 31.1 1.0

Calcium binding site 2 out of 8 in the Crystal Structure of A Small Conductance Potassium Channel Splice Variant Complexed with Calcium-Calmodulin


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of Crystal Structure of A Small Conductance Potassium Channel Splice Variant Complexed with Calcium-Calmodulin within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca1002 b:30.7 occ:1.00 OD1 A:ASP56 2.3 29.4 1.0 OD1 A:ASP58 2.4 37.9 1.0 OE1 A:GLU67 2.5 27.2 1.0 O A:THR62 2.5 22.4 1.0 OD1 A:ASN60 2.5 37.3 1.0 O A:HOH156 2.5 42.0 1.0 OE2 A:GLU67 2.6 29.2 1.0 CD A:GLU67 2.9 22.7 1.0 O A:HOH196 3.3 40.0 1.0 CG A:ASP58 3.4 51.0 1.0 CG A:ASN60 3.4 42.1 1.0 CG A:ASP56 3.4 29.9 1.0 C A:THR62 3.7 32.9 1.0 OD2 A:ASP58 3.7 58.3 1.0 ND2 A:ASN60 3.9 45.0 1.0 OD2 A:ASP56 4.2 27.9 1.0 N A:ASN60 4.3 33.3 1.0 CB A:ASP56 4.3 30.5 1.0 N A:THR62 4.3 23.5 1.0 CA A:ASP56 4.3 27.5 1.0 CG A:GLU67 4.4 23.3 1.0 N A:ASP58 4.4 30.2 1.0 CA A:ILE63 4.4 22.2 1.0 N A:ASP64 4.4 20.7 1.0 CB A:ASN60 4.5 38.5 1.0 N A:ILE63 4.5 20.0 1.0 OG1 A:THR62 4.5 26.9 1.0 C A:ASP56 4.6 27.1 1.0 CB A:ASP58 4.6 35.4 1.0 N A:GLY59 4.6 28.8 1.0 CA A:THR62 4.6 22.8 1.0 N A:GLY61 4.8 29.7 1.0 CA A:ASN60 4.8 36.4 1.0 CA A:ASP58 4.8 33.3 1.0 N A:ALA57 4.8 28.6 1.0 C A:ASP58 4.9 36.2 1.0 C A:ILE63 4.9 28.5 1.0 OD2 A:ASP64 4.9 32.6 1.0 CG A:ASP64 4.9 28.6 1.0 C A:ASN60 5.0 36.9 1.0

Calcium binding site 3 out of 8 in the Crystal Structure of A Small Conductance Potassium Channel Splice Variant Complexed with Calcium-Calmodulin


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 3 of Crystal Structure of A Small Conductance Potassium Channel Splice Variant Complexed with Calcium-Calmodulin within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca1003 b:31.1 occ:1.00 O A:TYR99 2.2 27.0 1.0 OD1 A:ASP95 2.2 43.4 1.0 OD1 A:ASP93 2.3 26.1 1.0 OD1 A:ASN97 2.4 32.8 1.0 OE1 A:GLU104 2.5 31.1 1.0 O A:HOH157 2.5 35.3 1.0 OE2 A:GLU104 2.6 31.9 1.0 CD A:GLU104 2.9 29.1 1.0 CG A:ASP95 3.2 51.3 1.0 C A:TYR99 3.4 28.2 1.0 CG A:ASN97 3.4 33.2 1.0 CG A:ASP93 3.6 31.4 1.0 OD2 A:ASP95 3.6 47.9 1.0 N A:TYR99 4.1 27.5 1.0 ND2 A:ASN97 4.1 33.2 1.0 N A:ASN97 4.2 27.9 1.0 N A:ASP95 4.2 31.9 1.0 CA A:TYR99 4.3 30.5 1.0 N A:ILE100 4.3 33.2 1.0 CA A:ASP93 4.3 24.1 1.0 OD2 A:ASP93 4.4 24.7 1.0 CG A:GLU104 4.4 31.9 1.0 CB A:ASN97 4.4 34.0 1.0 O A:HOH247 4.4 51.8 1.0 C A:ASP93 4.4 28.5 1.0 CA A:ILE100 4.5 29.1 1.0 CB A:ASP93 4.5 24.1 1.0 CB A:ASP95 4.5 41.4 1.0 N A:GLY96 4.6 25.5 1.0 N A:LYS94 4.6 27.1 1.0 N A:SER101 4.6 39.9 1.0 CA A:ASN97 4.7 32.6 1.0 CA A:ASP95 4.7 35.6 1.0 CB A:TYR99 4.7 30.9 1.0 N A:GLY98 4.8 27.2 1.0 O A:ASP93 4.8 32.0 1.0 C A:ASP95 4.8 39.1 1.0 O A:HOH240 4.9 58.9 1.0 C A:ASN97 4.9 30.5 1.0

Calcium binding site 4 out of 8 in the Crystal Structure of A Small Conductance Potassium Channel Splice Variant Complexed with Calcium-Calmodulin


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 4 of Crystal Structure of A Small Conductance Potassium Channel Splice Variant Complexed with Calcium-Calmodulin within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca1004 b:40.2 occ:1.00 OD1 A:ASP129 2.3 47.0 1.0 OD1 A:ASP133 2.4 58.0 1.0 O A:GLN135 2.4 40.0 1.0 OE1 A:GLU140 2.4 43.3 1.0 OE2 A:GLU140 2.4 46.9 1.0 O A:HOH158 2.5 44.5 1.0 OD1 A:ASP131 2.5 55.2 1.0 CD A:GLU140 2.8 42.5 1.0 CG A:ASP131 3.3 61.8 1.0 CG A:ASP133 3.4 62.7 1.0 CG A:ASP129 3.5 54.4 1.0 C A:GLN135 3.5 35.1 1.0 OD2 A:ASP131 3.6 65.0 1.0 OD2 A:ASP133 3.9 59.4 1.0 N A:ASP131 4.2 55.3 1.0 N A:GLN135 4.2 43.4 1.0 CA A:ASP129 4.2 50.8 1.0 CG A:GLU140 4.3 44.3 1.0 N A:ASP133 4.3 53.7 1.0 OD2 A:ASP129 4.3 51.3 1.0 CB A:ASP129 4.3 54.4 1.0 N A:ASN137 4.4 34.1 1.0 CA A:VAL136 4.4 29.5 1.0 N A:VAL136 4.4 30.9 1.0 CA A:GLN135 4.4 37.3 1.0 N A:ILE130 4.5 58.0 1.0 N A:GLY132 4.5 69.7 1.0 CB A:ASP133 4.5 57.8 1.0 C A:ASP129 4.5 58.2 1.0 CB A:ASP131 4.6 58.7 1.0 CA A:ASP131 4.7 62.8 1.0 C A:ASP131 4.8 70.4 1.0 N A:GLY134 4.8 53.1 1.0 CA A:ASP133 4.8 56.2 1.0 C A:VAL136 4.9 23.5 1.0 CG A:ASN137 4.9 32.1 1.0 ND2 A:ASN137 4.9 36.2 1.0

Calcium binding site 5 out of 8 in the Crystal Structure of A Small Conductance Potassium Channel Splice Variant Complexed with Calcium-Calmodulin


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 5 of Crystal Structure of A Small Conductance Potassium Channel Splice Variant Complexed with Calcium-Calmodulin within 5.0Å range: probe atom residue distance (Å) B Occ B:Ca1001 b:22.7 occ:1.00 OD1 B:ASP20 2.2 23.5 1.0 O B:THR26 2.3 30.5 1.0 OD1 B:ASP24 2.4 19.8 1.0 OE2 B:GLU31 2.4 23.3 1.0 OD1 B:ASP22 2.4 21.8 1.0 O B:HOH153 2.5 21.9 1.0 OE1 B:GLU31 2.6 24.2 1.0 CD B:GLU31 2.8 21.0 1.0 CG B:ASP24 3.2 30.2 1.0 CG B:ASP22 3.4 30.1 1.0 CG B:ASP20 3.4 20.9 1.0 C B:THR26 3.5 25.1 1.0 OD2 B:ASP22 3.8 29.3 1.0 OD2 B:ASP24 3.8 33.0 1.0 OG1 B:THR26 4.0 31.2 1.0 N B:ASP24 4.1 26.4 1.0 N B:THR26 4.1 17.1 1.0 OD2 B:ASP20 4.2 22.5 1.0 CA B:ASP20 4.2 24.0 1.0 CB B:ASP24 4.2 23.0 1.0 N B:ASP22 4.3 25.7 1.0 CG B:GLU31 4.3 21.3 1.0 CB B:ASP20 4.3 27.0 1.0 CA B:THR26 4.4 19.0 1.0 C B:ASP20 4.4 25.5 1.0 N B:ILE27 4.5 18.8 1.0 N B:GLY23 4.5 22.6 1.0 CA B:ILE27 4.5 18.6 1.0 CB B:ASP22 4.5 29.0 1.0 CA B:ASP24 4.6 23.1 1.0 N B:LYS21 4.6 25.5 1.0 N B:GLY25 4.6 23.7 1.0 O B:HOH197 4.7 30.6 1.0 CA B:ASP22 4.7 27.4 1.0 CG2 B:THR28 4.8 25.0 1.0 C B:ASP22 4.8 23.9 1.0 C B:ASP24 4.8 26.9 1.0 CB B:THR26 4.9 27.0 1.0 N B:THR28 4.9 21.7 1.0 O B:ASP20 5.0 26.8 1.0

Calcium binding site 6 out of 8 in the Crystal Structure of A Small Conductance Potassium Channel Splice Variant Complexed with Calcium-Calmodulin


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 6 of Crystal Structure of A Small Conductance Potassium Channel Splice Variant Complexed with Calcium-Calmodulin within 5.0Å range: probe atom residue distance (Å) B Occ B:Ca1002 b:29.1 occ:1.00 OD1 B:ASP56 2.2 28.4 1.0 OD1 B:ASN60 2.4 36.9 1.0 O B:HOH157 2.4 40.8 1.0 O B:THR62 2.4 24.8 1.0 OD1 B:ASP58 2.4 35.6 1.0 OE1 B:GLU67 2.4 23.1 1.0 OE2 B:GLU67 2.5 29.6 1.0 CD B:GLU67 2.8 21.6 1.0 O B:HOH269 3.1 43.5 1.0 CG B:ASN60 3.3 39.6 1.0 CG B:ASP58 3.4 51.9 1.0 CG B:ASP56 3.4 27.3 1.0 C B:THR62 3.6 35.4 1.0 OD2 B:ASP58 3.8 59.3 1.0 ND2 B:ASN60 3.8 40.8 1.0 OD2 B:ASP56 4.2 28.5 1.0 N B:ASN60 4.2 28.5 1.0 CB B:ASP56 4.3 32.1 1.0 CG B:GLU67 4.3 21.1 1.0 N B:THR62 4.3 27.9 1.0 CA B:ASP56 4.3 28.8 1.0 CA B:ILE63 4.4 19.5 1.0 N B:ASP58 4.4 30.4 1.0 N B:ILE63 4.5 21.5 1.0 CB B:ASN60 4.5 33.9 1.0 N B:ASP64 4.5 19.7 1.0 N B:GLY59 4.5 25.0 1.0 CB B:ASP58 4.6 35.7 1.0 CA B:THR62 4.6 28.4 1.0 C B:ASP56 4.6 28.0 1.0 OG1 B:THR62 4.7 24.6 1.0 CA B:ASN60 4.7 34.0 1.0 CA B:ASP58 4.8 36.3 1.0 N B:GLY61 4.8 26.4 1.0 C B:ASP58 4.8 34.8 1.0 N B:ALA57 4.9 28.4 1.0 OD2 B:ASP64 4.9 31.3 1.0 C B:ILE63 4.9 20.3 1.0 CG B:ASP64 5.0 28.9 1.0 C B:ASN60 5.0 35.6 1.0

Calcium binding site 7 out of 8 in the Crystal Structure of A Small Conductance Potassium Channel Splice Variant Complexed with Calcium-Calmodulin


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 7 of Crystal Structure of A Small Conductance Potassium Channel Splice Variant Complexed with Calcium-Calmodulin within 5.0Å range: probe atom residue distance (Å) B Occ B:Ca1003 b:30.5 occ:1.00 O B:TYR99 2.3 35.7 1.0 OD1 B:ASP95 2.3 36.0 1.0 OD1 B:ASP93 2.4 25.4 1.0 OD1 B:ASN97 2.4 34.4 1.0 O B:HOH159 2.4 38.7 1.0 OE1 B:GLU104 2.5 33.5 1.0 OE2 B:GLU104 2.5 33.3 1.0 CD B:GLU104 2.8 31.7 1.0 CG B:ASP95 3.2 44.8 1.0 CG B:ASN97 3.4 34.9 1.0 C B:TYR99 3.4 35.0 1.0 OD2 B:ASP95 3.5 54.5 1.0 CG B:ASP93 3.6 32.7 1.0 ND2 B:ASN97 4.0 34.8 1.0 N B:TYR99 4.1 30.3 1.0 N B:ASN97 4.1 29.6 1.0 N B:ASP95 4.2 33.1 1.0 CA B:ASP93 4.3 30.4 1.0 CA B:TYR99 4.3 31.3 1.0 CG B:GLU104 4.4 32.4 1.0 C B:ASP93 4.4 30.0 1.0 OD2 B:ASP93 4.4 26.7 1.0 CB B:ASN97 4.4 35.0 1.0 N B:ILE100 4.4 35.8 1.0 CB B:ASP93 4.4 29.4 1.0 CB B:ASP95 4.4 42.5 1.0 O B:HOH223 4.5 46.1 1.0 N B:GLY96 4.5 30.2 1.0 CA B:ILE100 4.6 37.0 1.0 N B:LYS94 4.6 32.7 1.0 N B:SER101 4.6 33.4 1.0 CA B:ASN97 4.6 32.5 1.0 CA B:ASP95 4.7 37.1 1.0 CB B:TYR99 4.7 38.8 1.0 N B:GLY98 4.7 28.6 1.0 O B:ASP93 4.8 28.9 1.0 C B:ASP95 4.8 36.6 1.0 C B:ASN97 4.9 32.8 1.0 O B:HOH227 4.9 39.3 1.0 O B:HOH270 4.9 51.2 1.0

Calcium binding site 8 out of 8 in the Crystal Structure of A Small Conductance Potassium Channel Splice Variant Complexed with Calcium-Calmodulin


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 8 of Crystal Structure of A Small Conductance Potassium Channel Splice Variant Complexed with Calcium-Calmodulin within 5.0Å range: probe atom residue distance (Å) B Occ B:Ca1004 b:38.5 occ:1.00 OD1 B:ASP133 2.3 49.7 1.0 OD1 B:ASP131 2.3 54.4 1.0 OD1 B:ASP129 2.3 39.4 1.0 O B:GLN135 2.4 32.5 1.0 O B:HOH160 2.4 46.5 1.0 OE1 B:GLU140 2.4 42.5 1.0 OE2 B:GLU140 2.7 48.5 1.0 CD B:GLU140 2.9 48.0 1.0 CG B:ASP131 3.2 59.7 1.0 CG B:ASP133 3.3 57.2 1.0 CG B:ASP129 3.5 42.3 1.0 C B:GLN135 3.6 38.0 1.0 OD2 B:ASP131 3.6 60.3 1.0 OD2 B:ASP133 3.8 58.9 1.0 N B:ASP133 4.1 53.2 1.0 N B:GLN135 4.2 39.1 1.0 N B:ASP131 4.2 50.6 1.0 CA B:ASP129 4.2 45.3 1.0 OD2 B:ASP129 4.2 48.9 1.0 CB B:ASP129 4.3 49.8 1.0 CB B:ASP133 4.4 53.7 1.0 CG B:GLU140 4.4 44.0 1.0 N B:GLY132 4.4 60.3 1.0 CA B:VAL136 4.4 28.8 1.0 N B:VAL136 4.4 30.6 1.0 N B:ASN137 4.5 31.0 1.0 CA B:GLN135 4.5 34.3 1.0 CB B:ASP131 4.5 55.3 1.0 C B:ASP129 4.5 52.9 1.0 N B:ILE130 4.5 49.0 1.0 CA B:ASP131 4.6 57.2 1.0 N B:GLY134 4.6 50.8 1.0 CA B:ASP133 4.6 56.6 1.0 C B:ASP131 4.7 62.3 1.0 C B:ASP133 4.9 55.5 1.0 C B:VAL136 4.9 25.9 1.0 ND2 B:ASN137 5.0 35.7 1.0

M.Zhang, C.Abrams, L.Wang, A.Gizzi, L.He, R.Lin, Y.Chen, P.J.Loll, J.M.Pascal, J.F.Zhang. Structural Basis For Calmodulin As A Dynamic Calcium Sensor. Structure V. 20 911 2012.
ISSN: ISSN 0969-2126
PubMed: 22579256
DOI: 10.1016/J.STR.2012.03.019