Calcium in PDB 3t13: Crystal Structure of Staphylococcal Nuclease Variant Delta+Phs A69G at Cryogenic Temperature

All present enzymatic activity of Crystal Structure of Staphylococcal Nuclease Variant Delta+Phs A69G at Cryogenic Temperature:
3.1.31.1;

The structure of Crystal Structure of Staphylococcal Nuclease Variant Delta+Phs A69G at Cryogenic Temperature, PDB code: 3t13 was solved by B.M.Doctrow, J.L.Schlessman, B.Garcia-Moreno E., A.Heroux, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	49.85 / 1.80
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	46.595, 63.755, 49.874, 90.00, 91.92, 90.00
R / Rfree (%)	15.6 / 19.9

The binding sites of Calcium atom in the Crystal Structure of Staphylococcal Nuclease Variant Delta+Phs A69G at Cryogenic Temperature (pdb code 3t13). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 2 binding sites of Calcium where determined in the Crystal Structure of Staphylococcal Nuclease Variant Delta+Phs A69G at Cryogenic Temperature, PDB code: 3t13:
Jump to Calcium binding site number: 1; 2;

Calcium binding site 1 out of 2 in the Crystal Structure of Staphylococcal Nuclease Variant Delta+Phs A69G at Cryogenic Temperature


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Crystal Structure of Staphylococcal Nuclease Variant Delta+Phs A69G at Cryogenic Temperature within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca152 b:19.3 occ:1.00 O A:THR41 2.6 13.7 1.0 O3 A:PO4150 2.6 18.7 1.0 O A:HOH225 2.7 20.6 1.0 O A:HOH178 2.7 13.8 1.0 OE2 A:GLU43 2.8 21.0 1.0 OD2 A:ASP21 2.8 13.7 1.0 O4 A:PO4150 3.5 18.0 1.0 CG A:ASP21 3.6 14.3 1.0 CD A:GLU43 3.6 23.7 1.0 OD1 A:ASP21 3.7 14.6 1.0 P A:PO4150 3.7 16.6 1.0 C A:THR41 3.7 14.6 1.0 O A:HOH291 3.9 34.2 1.0 OG1 A:THR41 4.0 13.7 1.0 N A:THR41 4.1 13.8 1.0 O A:HOH187 4.1 34.0 1.0 OE1 A:GLU43 4.2 20.4 1.0 NH2 A:ARG35 4.2 12.4 1.0 CA A:THR41 4.4 12.2 1.0 CG A:GLU43 4.5 18.0 1.0 C A:PRO42 4.5 14.7 1.0 O2 A:PO4150 4.5 14.2 1.0 O A:PRO42 4.5 15.2 1.0 N A:GLU43 4.5 16.0 1.0 C A:ASP40 4.7 14.4 1.0 O A:HOH239 4.7 39.5 1.0 CA A:ASP40 4.7 14.0 1.0 O1 A:PO4150 4.8 15.1 1.0 N A:PRO42 4.8 13.5 1.0 CA A:GLU43 4.9 16.2 1.0 OD2 A:ASP19 4.9 18.6 1.0 CB A:THR41 4.9 14.0 1.0 CZ A:ARG35 4.9 16.4 1.0 CA A:PRO42 4.9 15.4 1.0

Calcium binding site 2 out of 2 in the Crystal Structure of Staphylococcal Nuclease Variant Delta+Phs A69G at Cryogenic Temperature


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of Crystal Structure of Staphylococcal Nuclease Variant Delta+Phs A69G at Cryogenic Temperature within 5.0Å range: probe atom residue distance (Å) B Occ B:Ca153 b:28.7 occ:0.70 OD2 B:ASP21 2.8 25.5 1.0 O B:HOH239 2.8 34.3 1.0 O B:THR41 2.9 29.1 1.0 OE2 B:GLU43 2.9 55.8 1.0 O B:HOH294 3.0 50.2 1.0 O3 B:PO4150 3.0 30.1 0.4 O B:HOH274 3.5 40.5 1.0 OG1 B:THR41 3.6 27.6 1.0 CG B:ASP21 3.7 19.8 1.0 N B:THR41 3.7 25.5 1.0 OD1 B:ASP21 3.8 22.6 1.0 C B:THR41 3.9 28.3 1.0 CD B:GLU43 4.0 63.1 1.0 NH2 B:ARG35 4.1 17.2 1.0 P B:PO4150 4.1 32.6 0.4 O4 B:PO4150 4.2 28.9 0.4 CA B:THR41 4.3 25.5 1.0 C B:ASP40 4.5 22.1 1.0 O B:HOH189 4.5 43.4 1.0 CA B:ASP40 4.5 23.4 1.0 O4 B:PO4150 4.5 23.5 0.6 CB B:THR41 4.6 27.0 1.0 O3 B:PO4150 4.6 31.1 0.6 O B:PRO42 4.7 33.9 1.0 CG B:GLU43 4.8 57.9 1.0 CZ B:ARG35 4.8 20.4 1.0 O2 B:PO4150 4.8 31.9 0.4 OE1 B:GLU43 4.8 66.6 1.0 OD1 B:ASP40 4.8 34.2 1.0 C B:PRO42 4.8 35.5 1.0 CB B:ASP40 4.9 25.0 1.0 NZ A:LYS70 5.0 34.5 1.0 N B:GLU43 5.0 40.2 1.0 N B:PRO42 5.0 29.1 1.0 P B:PO4150 5.0 27.0 0.6

B.M.Doctrow, J.L.Schlessman, B.Garcia-Moreno E.. Crystal Structure of Staphylococcal Nuclease Variant Delta+Phs A69G at Cryogenic Temperature To Be Published.