Calcium in PDB 3t27: Orthorhombic Trypsin (Bovine) in the Presence of Betaine

All present enzymatic activity of Orthorhombic Trypsin (Bovine) in the Presence of Betaine:
3.4.21.4;

The structure of Orthorhombic Trypsin (Bovine) in the Presence of Betaine, PDB code: 3t27 was solved by M.Venkat, C.Saxby, J.Cahn, D.Juers, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	20.62 / 1.95
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	55.104, 58.174, 68.943, 90.00, 90.00, 90.00
R / Rfree (%)	14.7 / 19.1

The binding sites of Calcium atom in the Orthorhombic Trypsin (Bovine) in the Presence of Betaine (pdb code 3t27). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total only one binding site of Calcium was determined in the Orthorhombic Trypsin (Bovine) in the Presence of Betaine, PDB code: 3t27:

Calcium binding site 1 out of 1 in the Orthorhombic Trypsin (Bovine) in the Presence of Betaine


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Orthorhombic Trypsin (Bovine) in the Presence of Betaine within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca246 b:37.9 occ:1.00 O A:HOH330 2.2 24.9 1.0 OE1 A:GLU70 2.2 26.2 1.0 O A:VAL75 2.3 21.3 1.0 O A:ASN72 2.4 15.7 1.0 O A:HOH327 2.5 21.0 1.0 OE2 A:GLU80 2.7 24.3 1.0 CD A:GLU70 3.4 24.2 1.0 C A:VAL75 3.4 20.7 1.0 C A:ASN72 3.5 15.3 1.0 CD A:GLU80 3.6 22.4 1.0 CG A:GLU80 3.8 23.1 1.0 OE2 A:GLU70 3.9 24.8 1.0 OE1 A:GLU77 3.9 40.2 1.0 CA A:VAL76 4.1 26.4 1.0 N A:ASN72 4.2 15.2 1.0 N A:VAL76 4.2 22.7 1.0 CG A:GLU77 4.2 37.5 1.0 N A:GLU77 4.2 30.9 1.0 N A:VAL75 4.3 16.9 1.0 N A:ASP71 4.3 15.9 1.0 CA A:ASN72 4.4 15.7 1.0 CA A:ILE73 4.4 15.7 1.0 N A:ILE73 4.4 14.0 1.0 CA A:VAL75 4.5 18.9 1.0 O A:HOH322 4.5 32.6 1.0 CA A:GLU70 4.5 17.0 1.0 CD A:GLU77 4.5 41.3 1.0 CG A:GLU70 4.6 19.1 1.0 O A:HOH349 4.6 37.6 1.0 CB A:GLU70 4.7 16.6 1.0 C A:ILE73 4.7 15.6 1.0 C A:VAL76 4.7 28.7 1.0 OE1 A:GLU80 4.7 22.8 1.0 CB A:ASN72 4.9 16.3 1.0 C A:ASP71 4.9 16.1 1.0 C A:GLU70 4.9 16.9 1.0 CB A:GLU77 4.9 34.4 1.0 N A:ASN74 5.0 15.0 1.0

H.Marshall, M.Venkat, N.S.Hti Lar Seng, J.Cahn, D.H.Juers. The Use of Trimethylamine N-Oxide As A Primary Precipitating Agent and Related Methylamine Osmolytes As Cryoprotective Agents For Macromolecular Crystallography. Acta Crystallogr.,Sect.D V. 68 69 2012.
ISSN: ISSN 0907-4449
PubMed: 22194335
DOI: 10.1107/S0907444911050360