Calcium in PDB 3t28: Tmao-Grown Trypsin (Bovine)-Previously Unreported Tetragonal Crystal Form

All present enzymatic activity of Tmao-Grown Trypsin (Bovine)-Previously Unreported Tetragonal Crystal Form:
3.4.21.4;

The structure of Tmao-Grown Trypsin (Bovine)-Previously Unreported Tetragonal Crystal Form, PDB code: 3t28 was solved by J.Cahn, M.Venkat, S.Saxby, D.Juers, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	20.15 / 2.80
Space group	P 41 21 2
Cell size a, b, c (Å), α, β, γ (°)	53.927, 53.927, 181.905, 90.00, 90.00, 90.00
R / Rfree (%)	18.2 / 24.8

The binding sites of Calcium atom in the Tmao-Grown Trypsin (Bovine)-Previously Unreported Tetragonal Crystal Form (pdb code 3t28). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total only one binding site of Calcium was determined in the Tmao-Grown Trypsin (Bovine)-Previously Unreported Tetragonal Crystal Form, PDB code: 3t28:

Calcium binding site 1 out of 1 in the Tmao-Grown Trypsin (Bovine)-Previously Unreported Tetragonal Crystal Form


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Tmao-Grown Trypsin (Bovine)-Previously Unreported Tetragonal Crystal Form within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca252 b:13.9 occ:1.00 OE1 A:GLU70 2.3 13.7 1.0 OE2 A:GLU80 2.3 16.7 1.0 O A:VAL75 2.3 13.3 1.0 O A:ASN72 2.3 12.1 1.0 O A:HOH313 2.4 2.0 1.0 O A:HOH315 3.0 13.3 1.0 CD A:GLU70 3.2 11.7 1.0 CD A:GLU80 3.4 19.8 1.0 C A:ASN72 3.4 12.0 1.0 C A:VAL75 3.5 13.6 1.0 OE2 A:GLU70 3.5 9.6 1.0 CG A:GLU80 3.8 17.2 1.0 N A:VAL75 4.2 13.3 1.0 N A:ASN72 4.2 11.2 1.0 CA A:ILE73 4.2 12.7 1.0 N A:GLU77 4.2 17.8 1.0 OE1 A:GLU77 4.3 18.6 1.0 N A:ILE73 4.3 11.9 1.0 CA A:VAL76 4.3 16.1 1.0 CA A:ASN72 4.3 12.0 1.0 N A:VAL76 4.3 14.7 1.0 CA A:VAL75 4.4 13.0 1.0 C A:ILE73 4.5 13.2 1.0 OE1 A:GLU80 4.5 21.4 1.0 CG A:GLU77 4.5 18.6 1.0 CG A:GLU70 4.6 10.2 1.0 O A:HOH306 4.6 4.8 1.0 N A:ASP71 4.6 10.7 1.0 C A:VAL76 4.7 17.0 1.0 CB A:ASN72 4.8 11.8 1.0 CA A:GLU70 4.8 9.7 1.0 N A:ASN74 4.8 13.5 1.0 O A:ILE73 4.8 13.2 1.0 CB A:GLU70 4.9 9.7 1.0 CB A:GLU77 4.9 18.7 1.0 C A:ASP71 4.9 11.1 1.0 CD A:GLU77 4.9 18.4 1.0

H.Marshall, M.Venkat, N.S.Hti Lar Seng, J.Cahn, D.H.Juers. The Use of Trimethylamine N-Oxide As A Primary Precipitating Agent and Related Methylamine Osmolytes As Cryoprotective Agents For Macromolecular Crystallography. Acta Crystallogr.,Sect.D V. 68 69 2012.
ISSN: ISSN 0907-4449
PubMed: 22194335
DOI: 10.1107/S0907444911050360