Calcium in PDB 3t29: Tmao-Grown Trigonal Trypsin (Bovine)

All present enzymatic activity of Tmao-Grown Trigonal Trypsin (Bovine):
3.4.21.4;

The structure of Tmao-Grown Trigonal Trypsin (Bovine), PDB code: 3t29 was solved by J.Cahn, M.Venkat, H.Marshall, D.Juers, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	19.56 / 1.75
Space group	P 31 2 1
Cell size a, b, c (Å), α, β, γ (°)	54.370, 54.370, 105.355, 90.00, 90.00, 120.00
R / Rfree (%)	14.6 / 19

The binding sites of Calcium atom in the Tmao-Grown Trigonal Trypsin (Bovine) (pdb code 3t29). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total only one binding site of Calcium was determined in the Tmao-Grown Trigonal Trypsin (Bovine), PDB code: 3t29:

Calcium binding site 1 out of 1 in the Tmao-Grown Trigonal Trypsin (Bovine)


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Tmao-Grown Trigonal Trypsin (Bovine) within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca246 b:9.0 occ:1.00 O A:VAL75 2.3 7.1 1.0 OE1 A:GLU70 2.3 9.2 1.0 O A:ASN72 2.3 7.2 1.0 OE2 A:GLU80 2.3 9.2 1.0 O A:HOH323 2.4 8.5 1.0 O A:HOH358 2.5 8.3 1.0 CD A:GLU70 3.4 9.0 1.0 CD A:GLU80 3.4 11.1 1.0 C A:VAL75 3.4 7.8 1.0 C A:ASN72 3.5 7.5 1.0 CG A:GLU80 3.7 9.8 1.0 OE2 A:GLU70 3.8 9.2 1.0 CA A:VAL76 4.1 8.2 1.0 CA A:ILE73 4.2 7.8 1.0 N A:GLU77 4.2 8.1 1.0 N A:VAL76 4.2 8.1 1.0 N A:VAL75 4.2 8.1 1.0 OE1 A:GLU77 4.3 11.8 1.0 N A:ILE73 4.3 7.0 1.0 N A:ASN72 4.4 8.2 1.0 CA A:VAL75 4.4 8.1 1.0 CG A:GLU77 4.4 10.4 1.0 C A:ILE73 4.5 8.1 1.0 CA A:ASN72 4.5 8.1 1.0 OE1 A:GLU80 4.5 9.3 1.0 N A:ASP71 4.5 8.5 1.0 O A:HOH319 4.5 11.2 1.0 CG A:GLU70 4.6 7.8 1.0 C A:VAL76 4.7 8.5 1.0 CA A:GLU70 4.7 8.6 1.0 CD A:GLU77 4.8 15.3 1.0 N A:ASN74 4.8 8.0 1.0 CB A:GLU70 4.8 7.8 1.0 O A:HOH350 4.8 12.2 1.0 CB A:GLU77 4.9 8.8 1.0 O A:ILE73 4.9 7.3 1.0 CB A:ASN72 4.9 8.5 1.0 C A:ASP71 5.0 8.4 1.0

H.Marshall, M.Venkat, N.S.Hti Lar Seng, J.Cahn, D.H.Juers. The Use of Trimethylamine N-Oxide As A Primary Precipitating Agent and Related Methylamine Osmolytes As Cryoprotective Agents For Macromolecular Crystallography. Acta Crystallogr.,Sect.D V. 68 69 2012.
ISSN: ISSN 0907-4449
PubMed: 22194335
DOI: 10.1107/S0907444911050360