Calcium in PDB 3tlm: Crystal Structure of Endoplasmic Reticulum CA2+-Atpase (Serca) From Bovine Muscle

All present enzymatic activity of Crystal Structure of Endoplasmic Reticulum CA2+-Atpase (Serca) From Bovine Muscle:
3.6.3.8;

The structure of Crystal Structure of Endoplasmic Reticulum CA2+-Atpase (Serca) From Bovine Muscle, PDB code: 3tlm was solved by R.Sacchetto, I.Bertipaglia, S.Giannetti, L.Cendron, F.Mascarello, E.Damiani, E.Carafoli, G.Zanotti, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	41.99 / 2.95
Space group	C 1 2 1
Cell size a, b, c (Å), α, β, γ (°)	156.580, 75.260, 151.800, 90.00, 108.30, 90.00
R / Rfree (%)	20.5 / 27.7

The structure of Crystal Structure of Endoplasmic Reticulum CA2+-Atpase (Serca) From Bovine Muscle also contains other interesting chemical elements:

Magnesium	(Mg)	1 atom
Potassium	(K)	1 atom

The binding sites of Calcium atom in the Crystal Structure of Endoplasmic Reticulum CA2+-Atpase (Serca) From Bovine Muscle (pdb code 3tlm). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 2 binding sites of Calcium where determined in the Crystal Structure of Endoplasmic Reticulum CA2+-Atpase (Serca) From Bovine Muscle, PDB code: 3tlm:
Jump to Calcium binding site number: 1; 2;

Calcium binding site 1 out of 2 in the Crystal Structure of Endoplasmic Reticulum CA2+-Atpase (Serca) From Bovine Muscle


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Crystal Structure of Endoplasmic Reticulum CA2+-Atpase (Serca) From Bovine Muscle within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca1003 b:61.3 occ:1.00 OE2 A:GLU309 2.7 60.5 1.0 OD1 A:ASN795 2.7 66.4 1.0 OD1 A:ASP799 2.7 60.9 1.0 O A:ALA305 2.8 86.4 1.0 O A:ILE307 2.9 0.7 1.0 OE1 A:GLU309 3.0 64.2 1.0 O A:VAL304 3.1 73.7 1.0 C A:ALA305 3.1 76.1 1.0 CD A:GLU309 3.2 0.1 1.0 CG A:ASP799 3.5 61.9 1.0 C A:ILE307 3.5 98.5 1.0 CA A:ALA305 3.6 59.3 1.0 CB A:ASP799 3.7 61.0 1.0 N A:ILE307 3.7 0.8 1.0 N A:ALA306 3.8 63.2 1.0 C A:VAL304 3.9 67.0 1.0 CG A:ASN795 3.9 98.3 1.0 C A:ALA306 4.0 0.8 1.0 N A:ALA305 4.1 64.1 1.0 ND2 A:ASN767 4.2 83.9 1.0 N A:PRO308 4.2 0.9 1.0 CA A:ALA306 4.2 0.0 1.0 CA A:ILE307 4.3 78.6 1.0 CA A:PRO308 4.4 0.6 1.0 N A:GLU309 4.4 67.8 1.0 O A:HOH1028 4.5 50.9 1.0 O A:ALA306 4.6 76.9 1.0 OD2 A:ASP799 4.6 0.9 1.0 CG A:GLU309 4.7 0.7 1.0 ND2 A:ASN795 4.8 72.7 1.0 CB A:ASN795 4.8 96.8 1.0 O A:ALA303 4.9 85.2 1.0 C A:PRO308 5.0 68.4 1.0

Calcium binding site 2 out of 2 in the Crystal Structure of Endoplasmic Reticulum CA2+-Atpase (Serca) From Bovine Muscle


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of Crystal Structure of Endoplasmic Reticulum CA2+-Atpase (Serca) From Bovine Muscle within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca1004 b:70.2 occ:1.00 O A:HOH1028 2.4 50.9 1.0 OE1 A:GLU770 2.7 67.8 1.0 OD1 A:ASN767 2.8 48.9 1.0 OD2 A:ASP799 2.8 0.9 1.0 OG1 A:THR798 2.9 73.9 1.0 OE2 A:GLU907 3.0 94.8 1.0 OE1 A:GLU907 3.1 56.2 1.0 CG A:ASP799 3.3 61.9 1.0 CD A:GLU907 3.3 83.6 1.0 OD1 A:ASP799 3.4 60.9 1.0 CB A:THR798 3.7 63.0 1.0 CD A:GLU770 3.8 0.4 1.0 CG A:ASN767 4.0 99.3 1.0 O A:THR798 4.0 55.1 1.0 C A:THR798 4.1 61.1 1.0 CB A:SER766 4.1 43.9 1.0 OE2 A:GLU770 4.2 85.7 1.0 N A:ASP799 4.3 82.7 1.0 CB A:ASP799 4.4 61.0 1.0 N A:ASN767 4.5 63.2 1.0 CA A:THR798 4.5 56.8 1.0 CA A:ASP799 4.6 85.5 1.0 CG A:GLU907 4.6 59.2 1.0 ND2 A:ASN767 4.7 83.9 1.0 CA A:ASN767 4.9 95.6 1.0 OH A:TYR762 4.9 71.2 1.0 OG A:SER766 4.9 76.3 1.0 O A:ALA305 4.9 86.4 1.0 O A:VAL794 4.9 61.2 1.0 CG2 A:THR798 4.9 78.2 1.0 CG A:GLU770 5.0 55.6 1.0 C A:SER766 5.0 67.6 1.0

R.Sacchetto, I.Bertipaglia, S.Giannetti, L.Cendron, F.Mascarello, E.Damiani, E.Carafoli, G.Zanotti. Crystal Structure of Sarcoplasmic Reticulum Ca(2+)-Atpase (Serca) From Bovine Muscle. J.Struct.Biol. V. 178 38 2012.
ISSN: ISSN 1047-8477
PubMed: 22387132
DOI: 10.1016/J.JSB.2012.02.008