Calcium in PDB 3uf5: Crystal Structure of the Mouse Colony-Stimulating Factor 1 (Mcsf-1) Cytokine

The structure of Crystal Structure of the Mouse Colony-Stimulating Factor 1 (Mcsf-1) Cytokine, PDB code: 3uf5 was solved by J.Elegheert, N.Bracke, A.Bekaert, S.N.Savvides, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	39.46 / 2.80
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	36.320, 68.690, 144.620, 90.00, 90.00, 90.00
R / Rfree (%)	21 / 25.8

The binding sites of Calcium atom in the Crystal Structure of the Mouse Colony-Stimulating Factor 1 (Mcsf-1) Cytokine (pdb code 3uf5). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 4 binding sites of Calcium where determined in the Crystal Structure of the Mouse Colony-Stimulating Factor 1 (Mcsf-1) Cytokine, PDB code: 3uf5:
Jump to Calcium binding site number: 1; 2; 3; 4;

Calcium binding site 1 out of 4 in the Crystal Structure of the Mouse Colony-Stimulating Factor 1 (Mcsf-1) Cytokine


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Crystal Structure of the Mouse Colony-Stimulating Factor 1 (Mcsf-1) Cytokine within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca200 b:69.5 occ:1.00 OE1 A:GLU5 2.4 61.8 1.0 OE2 A:GLU5 3.0 56.1 1.0 CD A:GLU5 3.0 58.5 1.0 CG A:GLU5 4.5 56.1 1.0

Calcium binding site 2 out of 4 in the Crystal Structure of the Mouse Colony-Stimulating Factor 1 (Mcsf-1) Cytokine


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of Crystal Structure of the Mouse Colony-Stimulating Factor 1 (Mcsf-1) Cytokine within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca201 b:95.4 occ:1.00 ND1 A:HIS9 3.1 67.8 1.0 O A:HIS9 3.1 55.1 1.0 CG A:HIS9 3.6 65.6 1.0 CB A:HIS9 3.7 60.4 1.0 C A:HIS9 3.9 55.1 1.0 CE1 A:HIS9 4.0 67.6 1.0 CA A:HIS9 4.5 55.6 1.0 CD2 A:HIS9 4.7 66.3 1.0 NE2 A:HIS9 4.8 65.8 1.0 N A:MET10 4.9 51.6 1.0

Calcium binding site 3 out of 4 in the Crystal Structure of the Mouse Colony-Stimulating Factor 1 (Mcsf-1) Cytokine


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 3 of Crystal Structure of the Mouse Colony-Stimulating Factor 1 (Mcsf-1) Cytokine within 5.0Å range: probe atom residue distance (Å) B Occ B:Ca200 b:92.0 occ:1.00 OG1 B:THR105 2.4 80.7 1.0 OE2 B:GLU36 3.8 0.6 1.0 CB B:THR105 3.8 77.7 1.0 N B:THR105 4.3 75.7 1.0 OE1 B:GLU36 4.3 98.5 1.0 CD B:GLU36 4.4 96.4 1.0 CA B:THR105 4.6 74.5 1.0 CG2 B:THR105 4.7 77.0 1.0 C B:ARG104 4.9 73.8 1.0

Calcium binding site 4 out of 4 in the Crystal Structure of the Mouse Colony-Stimulating Factor 1 (Mcsf-1) Cytokine


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 4 of Crystal Structure of the Mouse Colony-Stimulating Factor 1 (Mcsf-1) Cytokine within 5.0Å range: probe atom residue distance (Å) B Occ B:Ca201 b:99.8 occ:1.00 O B:ASN138 2.9 49.2 1.0 OD1 B:ASN138 3.1 43.5 1.0 C B:ASN138 3.3 44.0 1.0 CG B:ASN138 3.3 41.1 1.0 N B:ASN140 3.4 43.7 1.0 N B:ASN141 3.5 47.4 1.0 CB B:ASN138 3.6 39.1 1.0 N B:CYS139 3.7 42.6 1.0 ND2 B:ASN141 3.8 58.7 1.0 C B:CYS139 3.8 44.5 1.0 CA B:CYS139 3.9 45.5 1.0 CG B:ASN141 4.0 57.4 1.0 CB B:ASN141 4.0 53.6 1.0 CA B:ASN138 4.0 41.5 1.0 ND2 B:ASN138 4.1 42.3 1.0 CA B:ASN140 4.1 45.6 1.0 CB B:ASN140 4.2 46.9 1.0 C B:ASN140 4.3 47.5 1.0 CA B:ASN141 4.4 49.6 1.0 OD1 B:ASN141 4.7 59.1 1.0 O B:CYS139 4.7 45.3 1.0 O B:HOH153 4.9 56.9 1.0

J.Elegheert, N.Bracke, P.Pouliot, I.Gutsche, A.V.Shkumatov, N.Tarbouriech, K.Verstraete, A.Bekaert, W.P.Burmeister, D.I.Svergun, B.N.Lambrecht, B.Vergauwen, S.N.Savvides. Allosteric Competitive Inactivation of Hematopoietic Csf-1 Signaling By the Viral Decoy Receptor BARF1 Nat.Struct.Mol.Biol. V. 19 938 2012.
ISSN: ISSN 1545-9993
PubMed: 22902366
DOI: 10.1038/NSMB.2367