Calcium in PDB 3v20: Crystal Structure of Type Iif Restriction Endonuclease BSE634I with Cognate Dna

All present enzymatic activity of Crystal Structure of Type Iif Restriction Endonuclease BSE634I with Cognate Dna:
3.1.21.4;

The structure of Crystal Structure of Type Iif Restriction Endonuclease BSE634I with Cognate Dna, PDB code: 3v20 was solved by E.N.Manakova, S.Grazulis, D.Golovenko, G.Tamulaitiene, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	55.56 / 2.35
Space group	P 21 21 2
Cell size a, b, c (Å), α, β, γ (°)	71.937, 87.387, 125.127, 90.00, 90.00, 90.00
R / Rfree (%)	24 / 29.3

The structure of Crystal Structure of Type Iif Restriction Endonuclease BSE634I with Cognate Dna also contains other interesting chemical elements:

Chlorine

(Cl)

1 atom

The binding sites of Calcium atom in the Crystal Structure of Type Iif Restriction Endonuclease BSE634I with Cognate Dna (pdb code 3v20). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 2 binding sites of Calcium where determined in the Crystal Structure of Type Iif Restriction Endonuclease BSE634I with Cognate Dna, PDB code: 3v20:
Jump to Calcium binding site number: 1; 2;

Calcium binding site 1 out of 2 in the Crystal Structure of Type Iif Restriction Endonuclease BSE634I with Cognate Dna


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Crystal Structure of Type Iif Restriction Endonuclease BSE634I with Cognate Dna within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca302 b:36.4 occ:1.00 OP1 C:DC6 2.1 20.4 1.0 OD2 A:ASP146 2.3 18.8 1.0 O A:LEU197 2.4 14.0 1.0 O A:HOH409 2.7 13.6 1.0 CG A:ASP146 3.1 21.1 1.0 OD1 A:ASP146 3.3 22.3 1.0 P C:DC6 3.4 15.4 1.0 C A:LEU197 3.4 14.6 1.0 N A:LEU197 3.6 15.0 1.0 O5' C:DC6 3.9 17.9 1.0 C5' C:DC6 3.9 16.0 1.0 CA A:LEU197 4.0 14.6 1.0 OE2 A:GLU80 4.2 27.4 1.0 OP2 C:DC6 4.2 18.0 1.0 NZ A:LYS198 4.4 11.2 1.0 CG A:LYS198 4.4 14.6 1.0 CB A:LEU197 4.5 14.8 1.0 O3' C:DA5 4.5 16.0 1.0 OP2 C:DC7 4.5 16.3 1.0 CB A:ASP146 4.5 20.4 1.0 N A:LYS198 4.5 15.1 1.0 C A:GLY196 4.6 15.2 1.0 O A:HOH404 4.7 19.2 1.0 CA A:LYS198 4.9 14.8 1.0 CA A:GLY196 4.9 15.3 1.0

Calcium binding site 2 out of 2 in the Crystal Structure of Type Iif Restriction Endonuclease BSE634I with Cognate Dna


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of Crystal Structure of Type Iif Restriction Endonuclease BSE634I with Cognate Dna within 5.0Å range: probe atom residue distance (Å) B Occ B:Ca301 b:42.9 occ:1.00 OD2 B:ASP146 2.3 37.2 1.0 OP1 D:DC6 2.3 29.3 1.0 O B:HOH401 2.4 40.9 1.0 O B:LEU197 2.5 29.8 1.0 CG B:ASP146 3.2 37.6 1.0 OD1 B:ASP146 3.4 37.2 1.0 P D:DC6 3.4 29.5 1.0 C B:LEU197 3.5 30.6 1.0 N B:LEU197 3.7 31.2 1.0 O5' D:DC6 3.7 28.4 1.0 C5' D:DC6 3.9 27.9 1.0 OE2 B:GLU80 4.1 44.1 1.0 CA B:LEU197 4.1 31.4 1.0 OP2 D:DC6 4.1 28.5 1.0 NZ B:LYS198 4.2 25.8 1.0 CG B:LYS198 4.4 28.4 1.0 OP2 D:DC7 4.5 25.1 1.0 N B:LYS198 4.6 30.7 1.0 C B:GLY196 4.6 31.9 1.0 CB B:ASP146 4.6 37.6 1.0 O3' D:DA5 4.7 35.1 1.0 CB B:LEU197 4.7 32.2 1.0 CA B:GLY196 4.9 31.7 1.0 CA B:LYS198 4.9 29.9 1.0 CE B:LYS198 4.9 27.6 1.0

E.Manakova, S.Grazulis, M.Zaremba, G.Tamulaitiene, D.Golovenko, V.Siksnys. Structural Mechanisms of the Degenerate Sequence Recognition By BSE634I Restriction Endonuclease. Nucleic Acids Res. V. 40 6741 2012.
ISSN: ISSN 0305-1048
PubMed: 22495930
DOI: 10.1093/NAR/GKS300