Calcium in PDB 3w3d: Crystal Structure of Smooth Muscle G Actin Dnase I Complex

All present enzymatic activity of Crystal Structure of Smooth Muscle G Actin Dnase I Complex:
3.1.21.1;

The structure of Crystal Structure of Smooth Muscle G Actin Dnase I Complex, PDB code: 3w3d was solved by N.Sakabe, K.Sakabe, K.Sasaki, H.Kondo, M.Shimomur, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	10.00 / 1.80
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	42.000, 225.300, 77.400, 90.00, 90.00, 90.00
R / Rfree (%)	19.6 / n/a

The binding sites of Calcium atom in the Crystal Structure of Smooth Muscle G Actin Dnase I Complex (pdb code 3w3d). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 2 binding sites of Calcium where determined in the Crystal Structure of Smooth Muscle G Actin Dnase I Complex, PDB code: 3w3d:
Jump to Calcium binding site number: 1; 2;

Calcium binding site 1 out of 2 in the Crystal Structure of Smooth Muscle G Actin Dnase I Complex


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Crystal Structure of Smooth Muscle G Actin Dnase I Complex within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca402 b:29.8 occ:1.00 O2G A:ATP401 2.2 11.1 1.0 O A:HOH501 2.3 30.8 1.0 O A:HOH503 2.3 24.3 1.0 O A:HOH502 2.4 46.5 1.0 O A:HOH514 2.4 30.5 1.0 O2B A:ATP401 2.4 19.1 1.0 O A:HOH632 2.6 50.7 1.0 PG A:ATP401 3.6 20.6 1.0 PB A:ATP401 3.6 22.0 1.0 O3B A:ATP401 4.0 18.6 1.0 O2A A:ATP401 4.1 28.5 1.0 O3G A:ATP401 4.1 18.6 1.0 O3A A:ATP401 4.2 24.8 1.0 O A:HOH513 4.3 28.9 1.0 OD2 A:ASP153 4.3 33.6 1.0 OD1 A:ASP10 4.4 28.2 1.0 CA A:GLY12 4.4 15.7 1.0 OD2 A:ASP10 4.5 32.0 1.0 OE1 A:GLN136 4.5 17.2 1.0 PA A:ATP401 4.6 26.6 1.0 CD A:GLN136 4.6 15.9 1.0 NZ A:LYS17 4.7 23.2 1.0 OD1 A:ASP153 4.7 27.8 1.0 O1G A:ATP401 4.7 16.7 1.0 O A:HOH669 4.9 68.3 1.0 CG A:GLN136 4.9 13.2 1.0 CG A:ASP10 4.9 31.3 1.0 O1B A:ATP401 5.0 24.1 1.0 CG A:ASP153 5.0 27.0 1.0

Calcium binding site 2 out of 2 in the Crystal Structure of Smooth Muscle G Actin Dnase I Complex


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of Crystal Structure of Smooth Muscle G Actin Dnase I Complex within 5.0Å range: probe atom residue distance (Å) B Occ B:Ca308 b:15.7 occ:1.00 O B:THR205 2.3 14.1 1.0 O B:HOH408 2.3 23.9 1.0 O B:THR207 2.4 10.5 1.0 O B:THR203 2.4 10.6 1.0 OD2 B:ASP201 2.6 9.4 1.0 OD1 B:ASP201 2.6 2.0 1.0 OG1 B:THR203 2.7 23.2 1.0 CG B:ASP201 2.9 6.9 1.0 C B:THR203 3.5 12.6 1.0 C B:THR205 3.5 14.8 1.0 C B:THR207 3.6 8.8 1.0 CB B:THR203 3.9 15.0 1.0 CA B:THR203 4.1 12.9 1.0 N B:THR207 4.1 9.1 1.0 C B:ALA204 4.2 11.1 1.0 N B:THR205 4.2 10.3 1.0 N B:THR203 4.3 13.9 1.0 O B:ALA204 4.4 10.3 1.0 N B:SER206 4.4 14.7 1.0 C B:SER206 4.4 11.2 1.0 N B:ALA204 4.4 14.5 1.0 CA B:THR205 4.5 14.2 1.0 CB B:ASP201 4.5 2.7 1.0 CA B:THR207 4.5 8.6 1.0 CA B:SER206 4.5 15.1 1.0 N B:ASN208 4.6 7.5 1.0 O B:HOH419 4.6 15.2 1.0 CA B:ALA204 4.6 11.2 1.0 CA B:ASN208 4.7 4.5 1.0 OG1 B:THR205 4.7 15.1 1.0 OH B:TYR253 4.7 8.6 1.0 CE1 B:TYR253 4.9 11.9 1.0 O B:SER206 5.0 18.3 1.0

K.Sasaki, K.Sakabe, N.Sakabe, H.Kondo, M.Shimomur. Refined Structure and Solvent Network of Chicken Gizzard G-Actin Dnase 1 Complex at 1.8A Resolution Acta Crystallogr.,Sect.A V. 49 C111 1993.
ISSN: ISSN 0108-7673