Calcium in PDB 3x32: Crystal Structure of the Oxidized Form of the Solubilized Domain of Porcine Cytochrome B5 in Form 1 Crystal

The structure of Crystal Structure of the Oxidized Form of the Solubilized Domain of Porcine Cytochrome B5 in Form 1 Crystal, PDB code: 3x32 was solved by Y.Hirano, S.Kimura, T.Tamada, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	50.00 / 0.83
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	32.339, 39.279, 60.950, 90.00, 90.00, 90.00
R / Rfree (%)	10.7 / 12.7

The structure of Crystal Structure of the Oxidized Form of the Solubilized Domain of Porcine Cytochrome B5 in Form 1 Crystal also contains other interesting chemical elements:

Iron

(Fe)

1 atom

The binding sites of Calcium atom in the Crystal Structure of the Oxidized Form of the Solubilized Domain of Porcine Cytochrome B5 in Form 1 Crystal (pdb code 3x32). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 2 binding sites of Calcium where determined in the Crystal Structure of the Oxidized Form of the Solubilized Domain of Porcine Cytochrome B5 in Form 1 Crystal, PDB code: 3x32:
Jump to Calcium binding site number: 1; 2;

Calcium binding site 1 out of 2 in the Crystal Structure of the Oxidized Form of the Solubilized Domain of Porcine Cytochrome B5 in Form 1 Crystal


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Crystal Structure of the Oxidized Form of the Solubilized Domain of Porcine Cytochrome B5 in Form 1 Crystal within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca102 b:6.5 occ:1.00 OE1 A:GLU42 2.4 9.2 1.0 OE1 A:GLU48 2.5 8.5 1.0 O A:HOH309 2.5 6.7 0.9 OE2 A:GLU48 2.5 8.4 1.0 OE2 A:GLU42 2.5 9.8 1.0 CD A:GLU48 2.8 6.8 1.0 CD A:GLU42 2.9 8.5 1.0 CG A:GLU48 4.3 7.9 1.0 O A:HOH307 4.3 10.3 0.8 CG A:GLU42 4.4 10.7 1.0 HA A:GLU42 4.4 10.4 1.0 O A:HOH319 4.5 13.1 0.9 HG2 A:GLU48 4.6 9.4 1.0 O A:HOH503 4.6 11.2 0.3 O A:HOH356 4.7 13.9 0.9 NH2 A:ARG52 4.7 9.9 0.6 HG2 A:GLU42 4.8 12.9 1.0 O A:HOH303 4.8 8.3 1.0 HG3 A:GLU48 4.8 9.4 1.0 HG3 A:GLU42 4.8 12.9 1.0 NH2 A:ARG52 5.0 8.6 0.4

Calcium binding site 2 out of 2 in the Crystal Structure of the Oxidized Form of the Solubilized Domain of Porcine Cytochrome B5 in Form 1 Crystal


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of Crystal Structure of the Oxidized Form of the Solubilized Domain of Porcine Cytochrome B5 in Form 1 Crystal within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca103 b:9.0 occ:0.70 O A:HOH347 2.3 16.8 0.8 O A:HOH319 2.3 13.1 0.9 O A:GLU42 2.4 12.3 1.0 O A:HOH329 2.4 18.6 1.0 O A:HOH317 2.4 14.4 0.9 O A:HOH307 2.5 10.3 0.8 C A:GLU42 3.6 8.4 1.0 HB3 A:GLU42 3.7 11.8 1.0 HA A:GLU42 4.1 10.4 1.0 O A:HOH365 4.3 17.4 0.8 OE1 A:GLU42 4.3 9.2 1.0 CA A:GLU42 4.3 8.7 1.0 CB A:GLU42 4.5 9.8 1.0 N A:GLU43 4.6 6.6 0.5 O A:HOH309 4.7 6.7 0.9 CA A:GLU43 4.7 9.3 0.5 O A:HOH507 4.8 35.0 0.3 N A:GLU43 4.8 8.6 0.5 OE2 A:GLU48 4.8 8.4 1.0 OE1 A:GLU43 5.0 37.5 0.5

Y.Hirano, S.Kimura, T.Tamada. High-Resolution Crystal Structures of the Solubilized Domain of Porcine Cytochrome B5 Acta Crystallogr.,Sect.D V. 71 1572 2015.
ISSN: ISSN 0907-4449
DOI: 10.1107/S1399004715009438