Calcium in PDB 4b97: Biomass Sensing Modules From Putative Rsgi-Like Proteins of Clostridium Thermocellum Resemble Family 3 Carbohydrate- Binding Module of Cellulosome

The structure of Biomass Sensing Modules From Putative Rsgi-Like Proteins of Clostridium Thermocellum Resemble Family 3 Carbohydrate- Binding Module of Cellulosome, PDB code: 4b97 was solved by O.Yaniv, G.Fichman, L.J.W.Shimon, E.A.Bayer, R.Lamed, F.Frolow, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	42.331 / 1.28
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	34.479, 56.043, 64.594, 90.00, 90.00, 90.00
R / Rfree (%)	13.97 / 15.64

The binding sites of Calcium atom in the Biomass Sensing Modules From Putative Rsgi-Like Proteins of Clostridium Thermocellum Resemble Family 3 Carbohydrate- Binding Module of Cellulosome (pdb code 4b97). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total only one binding site of Calcium was determined in the Biomass Sensing Modules From Putative Rsgi-Like Proteins of Clostridium Thermocellum Resemble Family 3 Carbohydrate- Binding Module of Cellulosome, PDB code: 4b97:

Calcium binding site 1 out of 1 in the Biomass Sensing Modules From Putative Rsgi-Like Proteins of Clostridium Thermocellum Resemble Family 3 Carbohydrate- Binding Module of Cellulosome


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Biomass Sensing Modules From Putative Rsgi-Like Proteins of Clostridium Thermocellum Resemble Family 3 Carbohydrate- Binding Module of Cellulosome within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca1152 b:17.9 occ:1.00 O A:ASN117 2.3 21.7 1.0 OD1 A:ASP121 2.4 15.2 1.0 OD2 A:ASP48 2.4 21.5 1.0 OG1 A:THR46 2.4 16.8 1.0 O A:THR46 2.5 14.2 1.0 O A:HOH2051 2.5 18.0 1.0 OD1 A:ASP120 2.5 25.9 1.0 OD1 A:ASP48 2.6 21.2 1.0 CG A:ASP48 2.9 19.2 1.0 CG A:ASP121 3.4 14.4 1.0 C A:THR46 3.4 13.7 1.0 C A:ASN117 3.5 19.7 1.0 CG A:ASP120 3.6 28.9 1.0 CB A:THR46 3.6 15.5 1.0 OD2 A:ASP121 3.7 16.1 1.0 N A:THR46 3.8 12.8 1.0 CA A:THR46 3.8 13.8 1.0 OD2 A:ASP120 4.0 31.2 1.0 N A:ASN117 4.1 18.9 1.0 CA A:ASN117 4.2 19.9 1.0 CB A:ASN117 4.3 24.2 1.0 CB A:ASP48 4.3 18.1 1.0 N A:LYS118 4.5 21.4 1.0 N A:ILE47 4.6 12.6 1.0 CA A:LYS118 4.6 20.6 1.0 OD1 A:ASN117 4.7 36.9 1.0 N A:ASP121 4.7 17.0 1.0 CB A:ASP121 4.7 13.8 1.0 N A:ASP48 4.8 15.7 1.0 CG2 A:THR46 4.8 16.9 1.0 O A:HOH2053 4.8 50.9 1.0 C A:ILE47 4.9 14.4 1.0 CA A:ASP121 4.9 14.4 1.0 CB A:ASP120 5.0 26.8 1.0 C A:ASP120 5.0 22.7 1.0 CG A:ASN117 5.0 32.8 1.0

O.Yaniv, G.Fichman, I.Borovok, Y.Shoham, E.A.Bayer, R.Lamed, L.J.W.Shimon, F.Frolow. Fine-Structural Variance of Family 3 Carbohydrate-Binding Modules As Extracellular Biomass-Sensing Components of Clostridium Thermocellum Anti-Sigma(I) Factors. Acta Crystallogr.,Sect.D V. 70 522 2014.
ISSN: ISSN 0907-4449
PubMed: 24531486
DOI: 10.1107/S139900471302926X