Calcium in PDB 4bir: Ribonuclease T1: Free HIS92GLN Mutant

All present enzymatic activity of Ribonuclease T1: Free HIS92GLN Mutant:
3.1.27.3;

The structure of Ribonuclease T1: Free HIS92GLN Mutant, PDB code: 4bir was solved by J.Doumen, J.Steyaert, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	10.00 / 1.70
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	48.820, 46.530, 41.200, 90.00, 90.00, 90.00
R / Rfree (%)	18.7 / n/a

The binding sites of Calcium atom in the Ribonuclease T1: Free HIS92GLN Mutant (pdb code 4bir). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total only one binding site of Calcium was determined in the Ribonuclease T1: Free HIS92GLN Mutant, PDB code: 4bir:

Calcium binding site 1 out of 1 in the Ribonuclease T1: Free HIS92GLN Mutant


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Ribonuclease T1: Free HIS92GLN Mutant within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca105 b:10.7 occ:1.00 O A:HOH146 2.3 4.7 1.0 O A:HOH130 2.4 10.2 1.0 O A:HOH145 2.5 14.7 1.0 O A:HOH134 2.6 10.9 1.0 OD2 A:ASP15 2.6 4.1 1.0 OD1 A:ASP15 2.6 10.2 1.0 CG A:ASP15 3.0 2.0 1.0 CB A:ASP15 4.3 8.7 1.0 O A:HOH147 4.6 32.7 1.0 OG A:SER12 4.6 11.4 1.0 O A:CYS10 4.7 10.3 1.0 O A:HOH159 4.8 19.2 1.0 CA A:GLY65 4.8 13.1 1.0 O A:HOH113 4.8 5.6 1.0 O A:HOH212 4.9 37.1 1.0 O A:SER63 5.0 7.0 1.0

I.Zegers, P.Verhelst, H.W.Choe, J.Steyaert, U.Heinemann, W.Saenger, L.Wyns. Role of Histidine-40 in Ribonuclease T1 Catalysis: Three-Dimensionalstructures of the Partially Active HIS40LYS Mutant. Biochemistry V. 31 11317 1992.
ISSN: ISSN 0006-2960
PubMed: 1445870
DOI: 10.1021/BI00161A009