Calcium in PDB 4bpz: Crystal Structure of LAMA_E269S From Zobellia Galactanivorans in Complex with A Trisaccharide of 1,3-1,4-Beta-D-Glucan.

All present enzymatic activity of Crystal Structure of LAMA_E269S From Zobellia Galactanivorans in Complex with A Trisaccharide of 1,3-1,4-Beta-D-Glucan.:
3.2.1.39;

The structure of Crystal Structure of LAMA_E269S From Zobellia Galactanivorans in Complex with A Trisaccharide of 1,3-1,4-Beta-D-Glucan., PDB code: 4bpz was solved by A.Labourel, M.Jam, A.Jeudy, M.Czjzek, G.Michel, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	71.34 / 1.13
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	44.528, 76.485, 142.674, 90.00, 90.00, 90.00
R / Rfree (%)	14.241 / 17.452

The binding sites of Calcium atom in the Crystal Structure of LAMA_E269S From Zobellia Galactanivorans in Complex with A Trisaccharide of 1,3-1,4-Beta-D-Glucan. (pdb code 4bpz). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 2 binding sites of Calcium where determined in the Crystal Structure of LAMA_E269S From Zobellia Galactanivorans in Complex with A Trisaccharide of 1,3-1,4-Beta-D-Glucan., PDB code: 4bpz:
Jump to Calcium binding site number: 1; 2;

Calcium binding site 1 out of 2 in the Crystal Structure of LAMA_E269S From Zobellia Galactanivorans in Complex with A Trisaccharide of 1,3-1,4-Beta-D-Glucan.


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Crystal Structure of LAMA_E269S From Zobellia Galactanivorans in Complex with A Trisaccharide of 1,3-1,4-Beta-D-Glucan. within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca400 b:16.5 occ:0.80 O A:GLY189 2.3 18.7 1.0 O A:GLU145 2.4 16.1 1.0 OD1 A:ASP377 2.4 17.6 1.0 O A:HOH2018 2.4 17.9 1.0 O A:ASP377 2.4 14.3 1.0 OE2 A:GLU147 2.5 32.0 1.0 C A:ASP377 3.4 14.2 1.0 C A:GLY189 3.4 18.8 1.0 CG A:ASP377 3.5 17.6 1.0 C A:GLU145 3.5 14.7 1.0 CD A:GLU147 3.7 31.9 1.0 CA A:ASP377 3.9 14.0 1.0 CA A:GLY189 4.1 19.5 1.0 O A:HOH2321 4.2 38.0 1.0 CA A:GLU145 4.2 15.3 1.0 CB A:ASP377 4.3 14.9 1.0 O A:HOH2320 4.3 25.6 1.0 OD2 A:ASP377 4.3 19.4 1.0 CB A:PHE146 4.4 16.3 1.0 CG A:GLU147 4.4 28.2 1.0 N A:THR190 4.5 17.4 1.0 N A:PHE146 4.5 15.2 1.0 N A:TYR378 4.5 12.7 1.0 C A:PHE146 4.6 19.1 1.0 O A:PHE146 4.6 20.7 1.0 CB A:GLU145 4.6 16.1 1.0 CA A:THR190 4.7 15.8 1.0 CA A:PHE146 4.7 16.6 1.0 OE1 A:GLU147 4.7 41.1 1.0 OE1 A:GLU145 4.8 17.6 1.0 CB A:TYR378 4.8 13.5 1.0 CA A:TYR378 4.9 13.2 1.0 CG2 A:THR190 4.9 19.4 1.0 N A:GLU147 5.0 18.8 1.0

Calcium binding site 2 out of 2 in the Crystal Structure of LAMA_E269S From Zobellia Galactanivorans in Complex with A Trisaccharide of 1,3-1,4-Beta-D-Glucan.


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of Crystal Structure of LAMA_E269S From Zobellia Galactanivorans in Complex with A Trisaccharide of 1,3-1,4-Beta-D-Glucan. within 5.0Å range: probe atom residue distance (Å) B Occ B:Ca400 b:23.8 occ:0.80 O B:GLU145 2.2 25.6 1.0 O B:GLY189 2.3 24.2 1.0 O B:HOH2072 2.4 72.7 1.0 O B:ASP377 2.4 21.7 1.0 OD1 B:ASP377 2.4 26.4 1.0 O B:HOH2013 2.4 27.4 1.0 C B:ASP377 3.4 19.2 1.0 C B:GLY189 3.4 24.5 1.0 C B:GLU145 3.4 21.6 1.0 CG B:ASP377 3.5 21.9 1.0 CA B:ASP377 3.8 18.9 1.0 CA B:GLY189 4.0 27.5 1.0 CA B:GLU145 4.1 22.2 1.0 CB B:ASP377 4.3 21.9 1.0 OD2 B:ASP377 4.4 25.8 1.0 CG B:GLU147 4.4 35.6 1.0 CB B:PHE146 4.4 26.1 1.0 N B:THR190 4.4 25.2 1.0 O B:PHE146 4.5 26.4 1.0 N B:PHE146 4.5 23.9 1.0 N B:TYR378 4.5 20.4 1.0 CB B:GLU145 4.5 23.3 1.0 O B:HOH2242 4.5 37.2 1.0 C B:PHE146 4.6 28.4 1.0 OE2 B:GLU147 4.6 59.9 1.0 CA B:THR190 4.6 24.1 1.0 CA B:PHE146 4.7 24.6 1.0 CB B:TYR378 4.8 21.8 1.0 CG2 B:THR190 4.8 26.2 1.0 OE1 B:GLU145 4.8 28.1 1.0 CA B:TYR378 4.9 19.5 1.0

A.Labourel, M.Jam, A.Jeudy, J.H.Hehemann, M.Czjzek, G.Michel. The Beta-Glucanase Zglama From Zobellia Galactanivorans Evolved A Bent Active Site Adapted For Efficient Degradation of Algal Laminarin. J.Biol.Chem. V. 289 2027 2014.
ISSN: ISSN 0021-9258
PubMed: 24337571
DOI: 10.1074/JBC.M113.538843