Calcium in PDB 4bu4: Ribonuclease T1 Complex with 2'Gmp

All present enzymatic activity of Ribonuclease T1 Complex with 2'Gmp:
3.1.27.3;

The structure of Ribonuclease T1 Complex with 2'Gmp, PDB code: 4bu4 was solved by R.Loris, S.Devos, U.Langhorst, K.Decanniere, J.Bouckaert, D.Maes, T.R.Transue, J.Steyaert, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	10.00 / 1.80
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	40.500, 48.120, 50.840, 90.00, 90.00, 90.00
R / Rfree (%)	20 / 25.3

The binding sites of Calcium atom in the Ribonuclease T1 Complex with 2'Gmp (pdb code 4bu4). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total only one binding site of Calcium was determined in the Ribonuclease T1 Complex with 2'Gmp, PDB code: 4bu4:

Calcium binding site 1 out of 1 in the Ribonuclease T1 Complex with 2'Gmp


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Ribonuclease T1 Complex with 2'Gmp within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca105 b:25.0 occ:1.00 O A:HOH319 2.4 21.1 1.0 O A:HOH381 2.5 38.7 1.0 OD2 A:ASP15 2.5 8.3 1.0 O A:HOH313 2.5 11.7 1.0 O A:HOH315 2.5 18.3 1.0 O A:HOH314 2.5 16.7 1.0 OD1 A:ASP15 2.6 8.1 1.0 O A:HOH312 2.8 41.8 1.0 CG A:ASP15 2.9 8.1 1.0 CB A:ASP15 4.3 8.1 1.0 O A:HOH363 4.4 31.6 1.0 O A:HOH353 4.5 34.8 1.0 O A:CYS10 4.6 9.0 1.0 OG A:SER12 4.6 7.5 1.0 O A:HOH342 4.8 14.5 1.0 N A:SER12 4.9 7.7 1.0

R.Loris, U.Langhorst, S.De Vos, K.Decanniere, J.Bouckaert, D.Maes, T.R.Transue, J.Steyaert. Conserved Water Molecules in A Large Family of Microbial Ribonucleases. Proteins V. 36 117 1999.
ISSN: ISSN 0887-3585
PubMed: 10373011
DOI: 10.1002/(SICI)1097-0134(19990701)36:1<117::AID-PROT10>3.0.CO;2-H