Calcium in PDB 4cte: Crystal Structure of the Catalytic Domain of the Modular Laminarinase Zglamc Mutant E142S in Complex with A Thio- Oligosaccharide

Enzymatic activity of Crystal Structure of the Catalytic Domain of the Modular Laminarinase Zglamc Mutant E142S in Complex with A Thio- Oligosaccharide

All present enzymatic activity of Crystal Structure of the Catalytic Domain of the Modular Laminarinase Zglamc Mutant E142S in Complex with A Thio- Oligosaccharide:
3.2.1.39;

Protein crystallography data

The structure of Crystal Structure of the Catalytic Domain of the Modular Laminarinase Zglamc Mutant E142S in Complex with A Thio- Oligosaccharide, PDB code: 4cte was solved by A.Labourel, M.Jam, L.Legentil, B.Sylla, E.Ficko-Blean, J.H.Hehemann, V.Ferrieres, M.Czjzek, G.Michel, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 48.11 / 1.80
Space group P 21 21 21
Cell size a, b, c (Å), α, β, γ (°) 56.010, 93.930, 142.280, 90.00, 90.00, 90.00
R / Rfree (%) 17.718 / 22.221

Other elements in 4cte:

The structure of Crystal Structure of the Catalytic Domain of the Modular Laminarinase Zglamc Mutant E142S in Complex with A Thio- Oligosaccharide also contains other interesting chemical elements:

Chlorine (Cl) 2 atoms
Sodium (Na) 1 atom

Calcium Binding Sites:

The binding sites of Calcium atom in the Crystal Structure of the Catalytic Domain of the Modular Laminarinase Zglamc Mutant E142S in Complex with A Thio- Oligosaccharide (pdb code 4cte). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 2 binding sites of Calcium where determined in the Crystal Structure of the Catalytic Domain of the Modular Laminarinase Zglamc Mutant E142S in Complex with A Thio- Oligosaccharide, PDB code: 4cte:
Jump to Calcium binding site number: 1; 2;

Calcium binding site 1 out of 2 in 4cte

Go back to Calcium Binding Sites List in 4cte
Calcium binding site 1 out of 2 in the Crystal Structure of the Catalytic Domain of the Modular Laminarinase Zglamc Mutant E142S in Complex with A Thio- Oligosaccharide


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Crystal Structure of the Catalytic Domain of the Modular Laminarinase Zglamc Mutant E142S in Complex with A Thio- Oligosaccharide within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Ca260

b:39.9
occ:1.00
 O A:GLU32 2.3 37.0 1.0
O A:GLY70 2.3 42.4 1.0
OD1 A:ASP247 2.3 36.0 1.0
O A:ASP247 2.3 31.9 1.0
O A:HOH2010 2.4 44.4 1.0
O A:HOH2009 2.4 46.4 1.0
O A:HOH2047 2.5 48.3 1.0
C A:ASP247 3.4 33.5 1.0
C A:GLU32 3.5 38.8 1.0
CG A:ASP247 3.5 36.3 1.0
C A:GLY70 3.5 40.2 1.0
CA A:ASP247 3.8 32.4 1.0
CA A:GLY70 4.1 41.6 1.0
CA A:GLU32 4.1 40.8 1.0
CB A:ASP247 4.3 32.8 1.0
O A:PHE33 4.3 43.9 1.0
OD2 A:ASP247 4.3 38.3 1.0
CB A:GLU32 4.4 39.6 1.0
C A:PHE33 4.5 45.2 1.0
N A:PHE33 4.5 40.5 1.0
N A:TYR248 4.5 31.6 1.0
N A:ASN71 4.6 37.5 1.0
OD1 A:ASP34 4.7 62.0 1.0
CB A:PHE33 4.7 38.6 1.0
OE1 A:GLU32 4.7 41.8 1.0
CB A:TYR248 4.7 36.9 1.0
CA A:PHE33 4.8 41.5 1.0
CA A:ASN71 4.8 37.1 1.0
CA A:TYR248 4.9 35.5 1.0
N A:ASP34 4.9 50.0 1.0

Calcium binding site 2 out of 2 in 4cte

Go back to Calcium Binding Sites List in 4cte
Calcium binding site 2 out of 2 in the Crystal Structure of the Catalytic Domain of the Modular Laminarinase Zglamc Mutant E142S in Complex with A Thio- Oligosaccharide


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of Crystal Structure of the Catalytic Domain of the Modular Laminarinase Zglamc Mutant E142S in Complex with A Thio- Oligosaccharide within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Ca280

b:21.4
occ:1.00
 OD1 B:ASP247 2.3 19.4 1.0
O B:GLY70 2.3 22.1 1.0
O B:GLU32 2.3 19.7 1.0
O B:HOH2009 2.4 24.2 1.0
O B:ASP247 2.4 21.0 1.0
OXT B:ACT282 2.5 25.5 1.0
O B:ACT282 2.6 25.7 1.0
C B:ACT282 2.7 31.6 1.0
C B:ASP247 3.4 20.7 1.0
C B:GLY70 3.5 22.4 1.0
CG B:ASP247 3.5 21.0 1.0
C B:GLU32 3.5 21.9 1.0
CA B:ASP247 3.9 20.3 1.0
CA B:GLY70 4.1 21.9 1.0
CH3 B:ACT282 4.1 41.8 1.0
CA B:GLU32 4.2 21.6 1.0
CB B:ASP247 4.3 19.9 1.0
O B:PHE33 4.3 22.1 1.0
OD2 B:ASP247 4.4 24.2 1.0
C B:PHE33 4.4 22.1 1.0
O B:HOH2186 4.5 41.7 1.0
CB B:GLU32 4.5 20.9 1.0
N B:PHE33 4.5 22.1 1.0
N B:ASN71 4.5 22.4 1.0
N B:TYR248 4.6 19.6 1.0
CB B:PHE33 4.6 20.5 1.0
OE1 B:GLU32 4.7 24.9 1.0
OD1 B:ASP34 4.7 26.5 1.0
CB B:TYR248 4.7 18.7 1.0
CA B:PHE33 4.8 21.2 1.0
N B:ASP34 4.8 21.5 1.0
CA B:ASN71 4.8 19.6 1.0
CA B:TYR248 4.9 18.1 1.0

Reference:

A.Labourel, M.Jam, L.Legentil, B.Sylla, J.H.Hehemann, V.Ferrieres, M.Czjzek, G.Michel. Structural and Biochemical Characterization of the Laminarina Zglamc[GH16] From Zobellia Galactanivorans Suggests Preferred Recognition of Branched Laminarin Acta Crystallogr.,Sect.D V. 71 173 2015.
ISSN: ISSN 0907-4449
DOI: 10.1107/S139900471402450X
Page generated: Sat Dec 12 04:42:49 2020

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