Calcium in PDB 4efh: Acanthamoeba Actin Complex with Spir Domain D

The structure of Acanthamoeba Actin Complex with Spir Domain D, PDB code: 4efh was solved by C.Chen, M.Phillips, M.R.Sawaya, M.E.Quinlan, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	40.57 / 2.48
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	53.006, 71.791, 126.089, 90.00, 90.00, 90.00
R / Rfree (%)	16.5 / 20.7

The binding sites of Calcium atom in the Acanthamoeba Actin Complex with Spir Domain D (pdb code 4efh). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 2 binding sites of Calcium where determined in the Acanthamoeba Actin Complex with Spir Domain D, PDB code: 4efh:
Jump to Calcium binding site number: 1; 2;

Calcium binding site 1 out of 2 in the Acanthamoeba Actin Complex with Spir Domain D


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Acanthamoeba Actin Complex with Spir Domain D within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca401 b:40.8 occ:1.00 O1B A:ADP403 2.2 24.8 1.0 O A:HOH510 2.2 37.5 1.0 O A:HOH509 2.2 23.9 1.0 O A:HOH534 2.3 29.1 1.0 O A:HOH502 2.3 30.0 1.0 O A:HOH535 2.6 30.9 1.0 PB A:ADP403 3.5 21.1 1.0 O A:HOH553 3.8 42.4 1.0 O3B A:ADP403 3.8 15.5 1.0 OE1 A:GLN137 4.1 23.5 1.0 CA A:GLY13 4.2 26.5 1.0 O A:HOH536 4.2 23.2 1.0 O1A A:ADP403 4.3 17.1 1.0 O3A A:ADP403 4.3 22.6 1.0 O A:HOH551 4.3 19.2 1.0 OD1 A:ASP11 4.4 26.5 1.0 CD A:GLN137 4.4 27.5 1.0 NZ A:LYS18 4.4 27.4 1.0 OD2 A:ASP11 4.5 26.7 1.0 OD1 A:ASP154 4.5 39.3 1.0 O2B A:ADP403 4.6 24.8 1.0 OD2 A:ASP154 4.6 45.8 1.0 CG2 A:VAL339 4.8 20.1 1.0 CG A:GLN137 4.8 17.5 1.0 CG A:ASP11 4.8 25.8 1.0 PA A:ADP403 4.9 20.9 1.0 NE2 A:GLN137 4.9 16.0 1.0

Calcium binding site 2 out of 2 in the Acanthamoeba Actin Complex with Spir Domain D


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of Acanthamoeba Actin Complex with Spir Domain D within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca402 b:0.9 occ:1.00 O A:SER271 3.1 35.0 1.0 N A:SER265 3.3 36.8 1.0 CD A:PRO264 3.4 32.4 1.0 N A:PRO264 3.6 32.0 1.0 O A:MET269 3.8 48.7 1.0 CB A:SER265 3.8 41.2 1.0 CB A:PRO264 3.9 32.4 1.0 C A:PRO264 3.9 38.6 1.0 CA A:PRO264 4.0 31.5 1.0 OG A:SER265 4.0 49.3 1.0 CA A:SER265 4.0 37.7 1.0 CG A:PRO264 4.0 35.4 1.0 C A:GLN263 4.2 36.8 1.0 C A:SER271 4.3 37.0 1.0 CA A:GLN263 4.5 33.0 1.0 O A:GLU270 4.7 51.2 1.0 C A:GLU270 4.7 48.5 1.0 C A:MET269 4.9 47.8 1.0 O A:GLN263 4.9 37.7 1.0 N A:SER271 4.9 40.2 1.0 O A:PRO264 5.0 39.5 1.0

C.K.Chen, M.R.Sawaya, M.L.Phillips, E.Reisler, M.E.Quinlan. Multiple Forms of Spire-Actin Complexes and Their Functional Consequences. J.Biol.Chem. V. 287 10684 2012.
ISSN: ISSN 0021-9258
PubMed: 22334675
DOI: 10.1074/JBC.M111.317792