Calcium in PDB 4g0n: Crystal Structure of Wt H-Ras-Gppnhp Bound to the Rbd of Raf Kinase

All present enzymatic activity of Crystal Structure of Wt H-Ras-Gppnhp Bound to the Rbd of Raf Kinase:
2.7.11.1;

The structure of Crystal Structure of Wt H-Ras-Gppnhp Bound to the Rbd of Raf Kinase, PDB code: 4g0n was solved by S.K.Fetics, B.M.Kearney, G.Buhrman, C.Mattos, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	32.37 / 2.45
Space group	P 3 2 1
Cell size a, b, c (Å), α, β, γ (°)	90.440, 90.440, 92.703, 90.00, 90.00, 120.00
R / Rfree (%)	18.1 / 22.9

The structure of Crystal Structure of Wt H-Ras-Gppnhp Bound to the Rbd of Raf Kinase also contains other interesting chemical elements:

Magnesium

(Mg)

1 atom

The binding sites of Calcium atom in the Crystal Structure of Wt H-Ras-Gppnhp Bound to the Rbd of Raf Kinase (pdb code 4g0n). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 2 binding sites of Calcium where determined in the Crystal Structure of Wt H-Ras-Gppnhp Bound to the Rbd of Raf Kinase, PDB code: 4g0n:
Jump to Calcium binding site number: 1; 2;

Calcium binding site 1 out of 2 in the Crystal Structure of Wt H-Ras-Gppnhp Bound to the Rbd of Raf Kinase


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Crystal Structure of Wt H-Ras-Gppnhp Bound to the Rbd of Raf Kinase within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca202 b:57.8 occ:1.00 O A:ACT205 2.2 78.2 1.0 O A:TYR137 2.4 48.6 1.0 O A:HOH345 2.4 42.0 1.0 O A:ASP107 2.4 74.1 1.0 O A:HOH346 2.4 53.4 1.0 OXT A:ACT205 2.5 67.2 1.0 C A:ACT205 2.7 73.8 1.0 O A:HOH347 2.9 56.2 1.0 C A:ASP107 3.4 64.6 1.0 C A:TYR137 3.5 41.2 1.0 CA A:ASP108 4.1 63.9 1.0 N A:ASP108 4.2 57.8 1.0 NH2 A:ARG97 4.2 64.9 1.0 CH3 A:ACT205 4.2 67.9 1.0 CA A:TYR137 4.3 39.1 1.0 CA A:ASP107 4.4 70.3 1.0 O A:HOH348 4.5 63.4 1.0 N A:GLY138 4.6 32.0 1.0 N A:VAL109 4.6 52.6 1.0 CB A:TYR137 4.7 32.8 1.0 CB A:ASP107 4.8 82.3 1.0 CA A:GLY138 4.8 30.5 1.0 O A:VAL109 4.8 43.6 1.0 C A:ASP108 4.8 51.7 1.0 CG2 A:ILE139 4.8 26.6 1.0 CE A:MET111 4.9 48.8 1.0 OD1 A:ASP108 5.0 0.9 1.0

Calcium binding site 2 out of 2 in the Crystal Structure of Wt H-Ras-Gppnhp Bound to the Rbd of Raf Kinase


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of Crystal Structure of Wt H-Ras-Gppnhp Bound to the Rbd of Raf Kinase within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca204 b:44.3 occ:0.33 O A:HOH332 2.4 39.9 1.0 O A:HOH333 2.5 43.9 1.0 O A:HOH363 3.6 97.9 0.3 O A:HOH357 4.2 39.7 1.0 O A:THR124 4.3 30.6 1.0 OE1 A:GLN129 4.7 38.7 1.0 NE2 A:GLN129 4.9 55.0 1.0

S.K.Fetics, H.Guterres, B.M.Kearney, G.Buhrman, B.Ma, R.Nussinov, C.Mattos. Allosteric Effects of the Oncogenic RASQ61L Mutant on Raf-Rbd. Structure V. 23 505 2015.
ISSN: ISSN 0969-2126
PubMed: 25684575
DOI: 10.1016/J.STR.2014.12.017