Calcium in PDB 4gnk: Crystal Structure of Galphaq in Complex with Full-Length Human PLCBETA3

All present enzymatic activity of Crystal Structure of Galphaq in Complex with Full-Length Human PLCBETA3:
3.1.4.11;

The structure of Crystal Structure of Galphaq in Complex with Full-Length Human PLCBETA3, PDB code: 4gnk was solved by A.M.Lyon, J.J.G.Tesmer, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	19.99 / 4.00
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	91.423, 188.845, 293.857, 90.00, 90.00, 90.00
R / Rfree (%)	21.4 / 25.5

The structure of Crystal Structure of Galphaq in Complex with Full-Length Human PLCBETA3 also contains other interesting chemical elements:

Fluorine	(F)	8 atoms
Magnesium	(Mg)	2 atoms
Aluminium	(Al)	2 atoms

The binding sites of Calcium atom in the Crystal Structure of Galphaq in Complex with Full-Length Human PLCBETA3 (pdb code 4gnk). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 2 binding sites of Calcium where determined in the Crystal Structure of Galphaq in Complex with Full-Length Human PLCBETA3, PDB code: 4gnk:
Jump to Calcium binding site number: 1; 2;

Calcium binding site 1 out of 2 in the Crystal Structure of Galphaq in Complex with Full-Length Human PLCBETA3


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Crystal Structure of Galphaq in Complex with Full-Length Human PLCBETA3 within 5.0Å range: probe atom residue distance (Å) B Occ B:Ca1301 b:89.4 occ:1.00 OE1 B:GLU362 2.2 93.3 1.0 OD1 B:ASN333 2.4 0.7 1.0 OD2 B:ASP364 2.8 0.7 1.0 OE1 B:GLU413 2.8 1.0 1.0 OD1 B:ASP364 3.3 0.3 1.0 OE2 B:GLU413 3.4 0.9 1.0 CG B:ASN333 3.4 0.3 1.0 CG B:ASP364 3.4 0.1 1.0 CD B:GLU413 3.4 0.3 1.0 CD B:GLU362 3.5 95.6 1.0 ND2 B:ASN333 3.7 0.0 1.0 CE1 B:HIS332 4.0 0.9 1.0 OG1 B:THR579 4.1 0.9 1.0 OE2 B:GLU362 4.2 93.8 1.0 OH B:TYR335 4.3 1.0 1.0 CG B:GLU362 4.4 98.5 1.0 ND1 B:HIS332 4.6 0.1 1.0 CB B:ASN333 4.8 0.9 1.0 CG B:GLU413 4.8 0.9 1.0 CB B:ASP364 4.9 0.2 1.0 NE2 B:HIS332 4.9 0.3 1.0

Calcium binding site 2 out of 2 in the Crystal Structure of Galphaq in Complex with Full-Length Human PLCBETA3


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of Crystal Structure of Galphaq in Complex with Full-Length Human PLCBETA3 within 5.0Å range: probe atom residue distance (Å) B Occ D:Ca1301 b:0.9 occ:1.00 OE1 D:GLU362 2.3 0.3 1.0 OD1 D:ASN333 2.4 0.3 1.0 OD2 D:ASP364 2.7 0.6 1.0 OE1 D:GLU413 2.8 0.1 1.0 OD1 D:ASP364 3.3 0.9 1.0 OE2 D:GLU413 3.4 0.2 1.0 CG D:ASN333 3.4 0.8 1.0 CG D:ASP364 3.4 0.6 1.0 CD D:GLU413 3.4 0.1 1.0 CD D:GLU362 3.6 0.5 1.0 ND2 D:ASN333 3.6 0.8 1.0 OG1 D:THR579 4.0 0.9 1.0 CE1 D:HIS332 4.1 0.4 1.0 OH D:TYR335 4.2 0.4 1.0 OE2 D:GLU362 4.3 0.5 1.0 CG D:GLU362 4.5 0.4 1.0 ND1 D:HIS332 4.6 0.4 1.0 CB D:ASN333 4.8 0.0 1.0 CG D:GLU413 4.9 0.8 1.0 CB D:ASP364 4.9 0.5 1.0 CD2 D:HIS379 4.9 0.3 1.0 NE2 D:HIS332 4.9 0.6 1.0

A.M.Lyon, S.Dutta, C.A.Boguth, G.Skiniotis, J.J.Tesmer. Full-Length G Alpha (Q)-Phospholipase C-Beta 3 Structure Reveals Interfaces of the C-Terminal Coiled-Coil Domain. Nat.Struct.Mol.Biol. V. 20 355 2013.
ISSN: ISSN 1545-9993
PubMed: 23377541
DOI: 10.1038/NSMB.2497