Calcium in PDB 4gx2: Gsuk Channel Bound to Nad

The structure of Gsuk Channel Bound to Nad, PDB code: 4gx2 was solved by C.Kong, W.Zeng, S.Ye, L.Chen, D.B.Sauer, Y.Lam, M.G.Derebe, Y.Jiang, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	41.14 / 3.20
Space group	C 1 2 1
Cell size a, b, c (Å), α, β, γ (°)	232.548, 111.104, 164.561, 90.00, 134.78, 90.00
R / Rfree (%)	23 / 27

The structure of Gsuk Channel Bound to Nad also contains other interesting chemical elements:

Potassium	(K)	12 atoms
Zinc	(Zn)	4 atoms

The binding sites of Calcium atom in the Gsuk Channel Bound to Nad (pdb code 4gx2). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 4 binding sites of Calcium where determined in the Gsuk Channel Bound to Nad, PDB code: 4gx2:
Jump to Calcium binding site number: 1; 2; 3; 4;

Calcium binding site 1 out of 4 in the Gsuk Channel Bound to Nad


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Gsuk Channel Bound to Nad within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca607 b:89.0 occ:1.00 O A:GLU449 3.1 97.8 1.0 O B:THR183 3.1 96.8 1.0 OD1 B:ASN210 3.1 82.8 1.0 OG1 B:THR214 3.2 79.0 1.0 OD1 A:ASN450 3.2 74.3 1.0 OE1 A:GLN453 3.3 82.8 1.0 CD A:GLN453 3.6 82.7 1.0 CA A:ASN450 3.8 81.6 1.0 CG A:GLN453 3.8 79.2 1.0 CG A:ASN450 3.9 76.2 1.0 C A:GLU449 3.9 95.7 1.0 C B:THR183 4.0 97.0 1.0 CG B:ASN210 4.1 81.2 1.0 CG2 B:THR214 4.1 84.8 1.0 N A:ASN450 4.2 88.0 1.0 CB B:THR214 4.3 81.7 1.0 CB B:THR183 4.3 97.7 1.0 CB A:ASN450 4.3 77.1 1.0 ND2 B:ASN210 4.3 80.8 1.0 CB A:GLN453 4.4 76.9 1.0 NE2 A:GLN453 4.4 86.0 1.0 O B:ASN210 4.6 84.2 1.0 CA B:THR183 4.7 95.9 1.0 OG1 B:THR183 4.7 99.2 1.0 ND2 A:ASN450 4.7 77.4 1.0 C A:ASN450 4.9 80.0 1.0 N B:ASP184 4.9 98.1 1.0 CD1 A:ILE426 5.0 64.2 1.0

Calcium binding site 2 out of 4 in the Gsuk Channel Bound to Nad


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of Gsuk Channel Bound to Nad within 5.0Å range: probe atom residue distance (Å) B Occ B:Ca603 b:89.9 occ:1.00 O B:GLU449 3.1 94.4 1.0 OE1 B:GLN453 3.2 86.1 1.0 OD1 B:ASN450 3.3 77.0 1.0 CD B:GLN453 3.6 86.0 1.0 CG B:GLN453 3.7 82.5 1.0 CA B:ASN450 3.9 77.8 1.0 C B:GLU449 4.0 92.0 1.0 CG B:ASN450 4.1 77.1 1.0 CB B:GLN453 4.3 77.6 1.0 N B:ASN450 4.3 83.7 1.0 NE2 B:GLN453 4.4 88.2 1.0 CB B:ASN450 4.5 75.2 1.0 CG B:GLU449 4.8 0.0 1.0 C B:ASN450 5.0 76.3 1.0 CD1 B:ILE426 5.0 72.1 1.0

Calcium binding site 3 out of 4 in the Gsuk Channel Bound to Nad


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 3 of Gsuk Channel Bound to Nad within 5.0Å range: probe atom residue distance (Å) B Occ C:Ca607 b:87.4 occ:1.00 O C:THR183 3.1 92.2 1.0 OD1 C:ASN210 3.1 76.7 1.0 OG1 C:THR214 3.2 81.7 1.0 C C:THR183 4.0 92.5 1.0 CG C:ASN210 4.1 75.3 1.0 CG2 C:THR214 4.2 84.3 1.0 CB C:THR214 4.3 82.6 1.0 CB C:THR183 4.3 94.0 1.0 ND2 C:ASN210 4.3 76.4 1.0 CA C:THR183 4.7 91.9 1.0 O C:ASN210 4.8 78.2 1.0 OG1 C:THR183 4.8 95.9 1.0 N C:ASP184 4.9 92.8 1.0

Calcium binding site 4 out of 4 in the Gsuk Channel Bound to Nad


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 4 of Gsuk Channel Bound to Nad within 5.0Å range: probe atom residue distance (Å) B Occ C:Ca608 b:90.5 occ:1.00 O C:GLU449 3.0 87.8 1.0 O D:THR183 3.0 88.9 1.0 OD1 D:ASN210 3.1 62.7 1.0 OG1 D:THR214 3.1 79.9 1.0 OD1 C:ASN450 3.1 73.2 1.0 OE1 C:GLN453 3.2 94.6 1.0 CD C:GLN453 3.5 93.6 1.0 CG C:GLN453 3.5 88.2 1.0 CA C:ASN450 3.8 76.1 1.0 CG2 D:THR214 3.9 78.0 1.0 CB C:GLN453 4.0 81.1 1.0 C C:GLU449 4.0 86.2 1.0 CG C:ASN450 4.0 73.9 1.0 CB D:THR214 4.1 76.4 1.0 CG D:ASN210 4.1 66.0 1.0 C D:THR183 4.1 88.7 1.0 N C:ASN450 4.4 80.8 1.0 ND2 D:ASN210 4.4 67.7 1.0 CB C:ASN450 4.5 72.8 1.0 NE2 C:GLN453 4.5 95.7 1.0 CB D:THR183 4.6 92.0 1.0 O D:ASN210 4.6 65.9 1.0 C C:ASN450 4.8 76.3 1.0 CA D:THR183 4.8 88.2 1.0 CD1 C:ILE426 4.9 73.7 1.0 CA D:THR214 4.9 73.7 1.0 O C:ASN450 4.9 75.1 1.0

C.Kong, W.Zeng, S.Ye, L.Chen, D.B.Sauer, Y.Lam, M.G.Derebe, Y.Jiang. Distinct Gating Mechanisms Revealed By the Structures of A Multi-Ligand Gated K(+) Channel. Elife V. 1 00184 2012.
PubMed: 23240087
DOI: 10.7554/ELIFE.00184